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Rabbit Recombinant Monoclonal NDUFB4 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115), expandable thumbnail
  • Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115), expandable thumbnail
  • Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternative names

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, Complex I-B15, NADH-ubiquinone oxidoreductase B15 subunit, CI-B15, NDUFB4

Recommended products

Rabbit Recombinant Monoclonal NDUFB4 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR16240
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251115 is the carrier-free version of Anti-NDUFB4 antibody [EPR16240] ab192243.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NDUFB4 also known as NADH: ubiquinone oxidoreductase subunit B4 plays a role in the mitochondrial respiratory chain complex I. Weighing approximately 15 kDa NDUFB4 is expressed in mitochondria across various cell types. It couples electron transfer from NADH to ubiquinone thereby contributing to energy production process in cells. NDUFB4 is important in maintaining mitochondrial functionality due to its role in the electron transport chain.

Biological function summary

NADH: ubiquinone oxidoreductase subunit B4 is part of the NADH dehydrogenase complex I. This complex is involved in the initial step of the mitochondrial electron transport chain. NDUFB4 supports the bioenergetic functions of cells by facilitating redox reactions. It also helps establish the electrochemical gradient needed for ATP synthesis. This protein interacts synergistically with other subunits in complex I to ensure efficient energy conversion.

Pathways

NADH dehydrogenase complex I which includes NDUFB4 is central in oxidative phosphorylation. This process supports energy production by converting NADH and FADH2 into ATP. NDUFB4 is integrally linked with proteins like NDUFS2 and NDUFV1 within this pathway ensuring electron transport is seamless. Complex I contributes to metabolic processes like the TCA cycle by feeding the mitochondria with necessary energy intermediates.

Associated diseases and disorders

Malfunction of NDUFB4 in complex I links to mitochondrial diseases such as Leigh syndrome. This syndrome affects central nervous system development. Mitochondrial encephalopathies are another group where NDUFB4 involvement is observed. In these conditions proteins like NDUFA1 and mitochondrial DNA mutations can manifest similar neurodegenerative symptoms due to impaired oxidative phosphorylation. Therefore understanding NDUFB4’s function helps in identifying therapeutic interventions for these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115), expandable thumbnail

    Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115)

    This data was developed using Anti-NDUFB4 antibody [EPR16240] ab192243, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-NDUFB4 antibody [EPR16240] (Anti-NDUFB4 antibody [EPR16240] ab192243) at 1/20000 dilution

    All lanes: Human fetal heart lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115)

    This data was developed using Anti-NDUFB4 antibody [EPR16240] ab192243, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB4 with Anti-NDUFB4 antibody [EPR16240] ab192243 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115), expandable thumbnail

    Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115)

    This data was developed using Anti-NDUFB4 antibody [EPR16240] ab192243, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-NDUFB4 antibody [EPR16240] (Anti-NDUFB4 antibody [EPR16240] ab192243) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: Human cerebellum tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa

  • Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115), expandable thumbnail

    Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115)

    This data was developed using Anti-NDUFB4 antibody [EPR16240] ab192243, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-NDUFB4 antibody [EPR16240] (Anti-NDUFB4 antibody [EPR16240] ab192243) at 1/5000 dilution

    All lanes: Rat kidney tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115)

    This data was developed using Anti-NDUFB4 antibody [EPR16240] ab192243, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling NDUFB4 with Anti-NDUFB4 antibody [EPR16240] ab192243 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. (inset: negative control). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (ab251115)

    This data was developed using Anti-NDUFB4 antibody [EPR16240] ab192243, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A549 cells labeling NDUFB4 with Anti-NDUFB4 antibody [EPR16240] ab192243 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).The two negative controls are anti-NDUFB4 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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