AB251115

Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal NDUFB4 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, Complex I-B15, NADH-ubiquinone oxidoreductase B15 subunit, CI-B15, NDUFB4

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

This data was developed using ab192243, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized A549 cells labeling NDUFB4 with ab192243 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).

The two negative controls are anti-NDUFB4 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

This data was developed using ab192243, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB4 with ab192243 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

(inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

This data was developed using ab192243, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling NDUFB4 with ab192243 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

(inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

This data was developed using ab192243, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NDUFB4 antibody [EPR16240] (<a href='/en-us/products/primary-antibodies/ndufb4-antibody-epr16240-ab192243'>ab192243</a>) at 1/20000 dilution

All lanes:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

This data was developed using ab192243, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NDUFB4 antibody [EPR16240] (<a href='/en-us/products/primary-antibodies/ndufb4-antibody-epr16240-ab192243'>ab192243</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Human cerebellum tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

This data was developed using ab192243, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NDUFB4 antibody [EPR16240] (<a href='/en-us/products/primary-antibodies/ndufb4-antibody-epr16240-ab192243'>ab192243</a>) at 1/5000 dilution

All lanes:

Rat kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa

false

Unconjugated

Anti-NDUFB4 antibody [EPR16240]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16240

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab251115 is the carrier-free version of ab192243.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFB4 also known as NADH: ubiquinone oxidoreductase subunit B4 plays a role in the mitochondrial respiratory chain complex I. Weighing approximately 15 kDa NDUFB4 is expressed in mitochondria across various cell types. It couples electron transfer from NADH to ubiquinone thereby contributing to energy production process in cells. NDUFB4 is important in maintaining mitochondrial functionality due to its role in the electron transport chain.

Biological function summary

NADH: ubiquinone oxidoreductase subunit B4 is part of the NADH dehydrogenase complex I. This complex is involved in the initial step of the mitochondrial electron transport chain. NDUFB4 supports the bioenergetic functions of cells by facilitating redox reactions. It also helps establish the electrochemical gradient needed for ATP synthesis. This protein interacts synergistically with other subunits in complex I to ensure efficient energy conversion.

Pathways

NADH dehydrogenase complex I which includes NDUFB4 is central in oxidative phosphorylation. This process supports energy production by converting NADH and FADH2 into ATP. NDUFB4 is integrally linked with proteins like NDUFS2 and NDUFV1 within this pathway ensuring electron transport is seamless. Complex I contributes to metabolic processes like the TCA cycle by feeding the mitochondria with necessary energy intermediates.

Malfunction of NDUFB4 in complex I links to mitochondrial diseases such as Leigh syndrome. This syndrome affects central nervous system development. Mitochondrial encephalopathies are another group where NDUFB4 involvement is observed. In these conditions proteins like NDUFA1 and mitochondrial DNA mutations can manifest similar neurodegenerative symptoms due to impaired oxidative phosphorylation. Therefore understanding NDUFB4’s function helps in identifying therapeutic interventions for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

See full target information NDUFB4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:760 PubMed37993451

2023

The BRD4-SRPK2-SRSF2 signal modulates the splicing efficiency of ACSL3 pre-mRNA and influences erastin-induced ferroptosis in osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shun-Hong Luo,Jia-Ming Tian,Yi Chu,Hong-Yi Zhu,Jiang-Dong Ni,Jun Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

Western blot - Anti-NDUFB4 antibody [EPR16240] - BSA and Azide free (AB251115)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise