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AB230215

Anti-NDUFB5 antibody

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(3 Publications)

Rabbit Polyclonal NDUFB5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial aa 100 to C-terminus.

View Alternative Names

Complex I-SGDH, NADH-ubiquinone oxidoreductase SGDH subunit, CI-SGDH, NDUFB5

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB5 antibody (AB230215)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB5 antibody (AB230215)

Paraffin-embedded human kidney tissue stained for NDUFB5 using ab230215 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB5 antibody (AB230215)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB5 antibody (AB230215)

Paraffin-embedded human spleen tissue stained for NDUFB5 using ab230215 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB5 antibody (AB230215)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB5 antibody (AB230215)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NDUFB5 (green) using ab230215 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor®488-conjugated Goat anti-Rabbit IgG (H+L).

Western blot - Anti-NDUFB5 antibody (AB230215)
  • WB

Supplier Data

Western blot - Anti-NDUFB5 antibody (AB230215)

All lanes:

Western blot - Anti-NDUFB5 antibody (ab230215) at 1/500 dilution

Lane 1:

Mouse heart tissue lysate

Lane 2:

Mouse kidney tissue lysate

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 5:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43674

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NDUFB5 protein also known as NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5 is a part of the mitochondrial respiratory chain complex I. It has a molecular mass of approximately 22 kDa. This protein is expressed in tissues with high energy demands such as the brain and muscle. Complex I where NDUFB5 operates plays an important role in cellular respiration initiating the electron transport chain by transferring electrons from NADH to ubiquinone.
Biological function summary

The NDUFB5 protein functions as a subunit within a larger multi-protein enzyme complex called mitochondrial complex I. This complex consisting of 45 subunits is important for oxidative phosphorylation. NDUFB5 is involved in the transfer of electrons facilitating the generation of a proton gradient across the mitochondrial inner membrane. This proton gradient drives ATP production which is essential for energy supply in cells.

Pathways

NDUFB5 participates centrally in the oxidative phosphorylation pathway and is involved in the production of ATP from ADP. The protein's interaction with NADH and ubiquinone links it closely to cellular respiration processes. It collaborates directly with other complex I subunits such as NDUFA and NDUFS to ensure effective energy conversion. Changes in NDUFB5 function can disrupt these pathways affecting overall energy metabolism.

Changes in NDUFB5 activity often relate to mitochondrial disorders particularly those affecting muscle and neurological function such as Leigh syndrome. Mutations affecting its role in complex I can disrupt ATP production. NDUFB5’s dysfunction may connect with other complex I proteins like NDUFS2 leading to bioenergetic failure in cells. These disruptions often result in varied clinical effects highlighting the importance of NDUFB5 in health and disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
See full target information NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:643 PubMed38982516

2024

METTL3-mediated NDUFB5 m6A modification promotes cell migration and mitochondrial respiration to promote the wound healing of diabetic foot ulcer.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Xu Li,Yue Tao,Xiaojun Wang,Limeng Li,Jianjun Liu

Frontiers in oncology 12:985827 PubMed36818671

2023

Identification of NLE1/CDK1 axis as key regulator in the development and progression of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Xu,Lei Wang,Bin Mo,Xiao Xie,Rui Hu,Lianyong Jiang,Fengqing Hu,Fangbao Ding,Haibo Xiao

Oxidative medicine and cellular longevity 2023:4202861 PubMed36647431

2023

Psoraleae Fructus Ethanol Extract Induced Hepatotoxicity via Impaired Lipid Metabolism Caused by Disruption of Fatty Acid -Oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaojuan Guo,Yuanyuan Shi,Bingqian Jiang,Xiyi Peng,Lin Zhang,Can Tu,Ting Wang
View all publications
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