Anti-NDUFB7 antibody [EPR15903] - BSA and Azide free

• Flow Cyt

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Flow Cytometry - Anti-NDUFB7 antibody [EPR15903] - BSA and Azide free (AB250968)

This data was developed using ab188575, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labelling NDUFB7 with Purified ab188575 at 1 : 2000 dilution (1 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NDUFB7 antibody [EPR15903] - BSA and Azide free (AB250968)

This data was developed using ab188575, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling NDUFB7 with Purified ab188575 at 1 : 250 dilution (7.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB7 antibody [EPR15903] - BSA and Azide free (AB250968)

This data was developed using ab188575, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling NDUFB7 with Purified ab188575 at 1 : 550 dilution (3.47 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IP

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Immunoprecipitation - Anti-NDUFB7 antibody [EPR15903] - BSA and Azide free (AB250968)

This data was developed using ab188575, the same antibody clone in a different buffer formulation.
Purified ab188575 at 1 : 100 dilution (2μg) immunoprecipitating NDUFB7 in A431 whole cell lysate.

Lane 1 (input) : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate 10μg.
Lane 2 (+) : ab188575 + A431 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab188575 in A431 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)(ab131366) (1 : 1000 dilution) was used for Western blotting.

Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 16 kDa

All lanes:

Immunoprecipitation - Anti-NDUFB7 antibody [EPR15903] (<a href='/en-us/products/primary-antibodies/ndufb7-antibody-epr15903-ab188575'>ab188575</a>)

Predicted band size: 16 kDa

false

• WB

Unknown

Western blot - Anti-NDUFB7 antibody [EPR15903] - BSA and Azide free (AB250968)

This data was developed using ab188575, the same antibody clone in a different buffer formulation.

We are unsure about the nature of the extra bands.

All lanes:

Western blot - Anti-NDUFB7 antibody [EPR15903] (<a href='/en-us/products/primary-antibodies/ndufb7-antibody-epr15903-ab188575'>ab188575</a>) at 1/1000 dilution

Lane 1:

HL-60 (uman acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

Lane 2:

PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

false