Mouse Monoclonal NDUFB8 antibody. Suitable for WB, IHC-Fr and reacts with Mouse, Rat, Cow, Human samples. Cited in 203 publications.
Images
![Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (AB110242), expandable thumbnail](https://content.abcam.com/products/images/ndufb8-antibody-20e9dh10c12-ab110242--immunohistochemistry-frozen-sections-img82229.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (AB110242)")
![Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (AB110242), expandable thumbnail](https://content.abcam.com/products/images/ndufb8-antibody-20e9dh10c12-ab110242--western-blot-img36695.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (AB110242)")
Publications
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- Antioxidants (Basel, Switzerland) 12:2023Melatonin Alleviates the Impairment of Muscle Bioenergetics and Protein Quality Control Systems in Leptin-Deficiency-Induced Obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaiza Potes,Andrea Díaz-Luis,Juan C Bermejo-Millo,Zulema Pérez-Martínez,Beatriz de Luxán-Delgado,Adrian Rubio-González,Iván Menéndez-Valle,José Gutiérrez-Rodríguez,Juan J Solano,Beatriz Caballero,Ignacio Vega-Naredo,Ana Coto-MontesPubMed 38001815
- Communications biology 6:10782023A novel mouse model of mitochondrial disease exhibits juvenile-onset severe neurological impairment due to parvalbumin cell mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizaveta A Olkhova,Carla Bradshaw,Alasdair Blain,Debora Alvim,Doug M Turnbull,Fiona E N LeBeau,Yi Shiau Ng,Gráinne S Gorman,Nichola Z LaxPubMed 37872380
- International journal of molecular sciences 24:2023Characterizing Early Cardiac Metabolic Programming via 30% Maternal Nutrient Reduction during Fetal Development in a Non-Human Primate Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusana P Pereira,Mariana S Diniz,Ludgero C Tavares,Teresa Cunha-Oliveira,Cun Li,Laura A Cox,Mark J Nijland,Peter W Nathanielsz,Paulo J OliveiraPubMed 37894873
- Nature 622:627-6362023Apoptotic stress causes mtDNA release during senescence and drives the SASP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStella Victorelli,Hanna Salmonowicz,James Chapman,Helene Martini,Maria Grazia Vizioli,Joel S Riley,Catherine Cloix,Ella Hall-Younger,Jair Machado Espindola-Netto,Diana Jurk,Anthony B Lagnado,Lilian Sales Gomez,Joshua N Farr,Dominik Saul,Rebecca Reed,George Kelly,Madeline Eppard,Laura C Greaves,Zhixun Dou,Nicholas Pirius,Karolina Szczepanowska,Rebecca A Porritt,Huijie Huang,Timothy Y Huang,Derek A Mann,Claudio Akio Masuda,Sundeep Khosla,Haiming Dai,Scott H Kaufmann,Emmanouil Zacharioudakis,Evripidis Gavathiotis,Nathan K LeBrasseur,Xue Lei,Alva G Sainz,Viktor I Korolchuk,Peter D Adams,Gerald S Shadel,Stephen W G Tait,João F PassosPubMed 37821702
- Nature metabolism 5:1290-13022023Fatty acid oxidation organizes mitochondrial supercomplexes to sustain astrocytic ROS and cognition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrenda Morant-Ferrando,Daniel Jimenez-Blasco,Paula Alonso-Batan,Jesús Agulla,Rebeca Lapresa,Dario Garcia-Rodriguez,Sara Yunta-Sanchez,Irene Lopez-Fabuel,Emilio Fernandez,Peter Carmeliet,Angeles Almeida,Marina Garcia-Macia,Juan P BolañosPubMed 37460843
- Frontiers in cell and developmental biology 11:11792522023Hippocampal versus cortical deletion of cholinergic receptor muscarinic 1 in mice differentially affects post-translational modifications and supramolecular assembly of respiratory chain-associated proteins, mitochondrial ultrastructure, and respiration: implications in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammad Golam Sabbir,Mamiko Swanson,Robert C Speth,Benedict C AlbensiPubMed 37293125
- Biogerontology 24:391-4012023Early-onset caloric restriction alleviates ageing-associated steatohepatitis in male mice via restoring mitochondrial homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Hsien Chiang,Sin-Jin Li,Yu-Han Lin,Pei-Yu Wang,Pu-Sheng Hsu,Shau-Ping Lin,Ting-Chia Chiang,Ching-Yi ChenPubMed 36802043
- Molecular metabolism 69:1016832023Impairment of adrenergically-regulated thermogenesis in brown fat of obesity-resistant mice is compensated by non-shivering thermogenesis in skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPetra Janovska,Petr Zouhar,Kristina Bardova,Jakub Otahal,Marek Vrbacky,Tomas Mracek,Katerina Adamcova,Lucie Lenkova,Jiri Funda,Tomas Cajka,Zdenek Drahota,Sara Stanic,Arild C Rustan,Olga Horakova,Josef Houstek,Martin Rossmeisl,Jan KopeckyPubMed 36720306
- Nature communications 14:3122023A FRET-based respirasome assembly screen identifies spleen tyrosine kinase as a target to improve muscle mitochondrial respiration and exercise performance in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmi Kobayashi,Kotaro Azuma,Toshihiko Takeiwa,Toshimori Kitami,Kuniko Horie,Kazuhiro Ikeda,Satoshi InouePubMed 36697396
- Nature biomedical engineering 7:692-7032022A library of base editors for the precise ablation of all protein-coding genes in the mouse mitochondrial genome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPedro Silva-Pinheiro,Christian D Mutti,Lindsey Van Haute,Christopher A Powell,Pavel A Nash,Keira Turner,Michal MinczukPubMed 36470976
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-Fr | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Cow | Tested | Expected |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5 µg/mL | Notes - |
Species Rat | Dilution info 0.5 µg/mL | Notes - |
Species Cow | Dilution info 0.5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Alternative names
Complex I-ASHI, NADH-ubiquinone oxidoreductase ASHI subunit, CI-ASHI, NDUFB8
Recommended products
Mouse Monoclonal NDUFB8 antibody. Suitable for WB, IHC-Fr and reacts with Mouse, Rat, Cow, Human samples. Cited in 203 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 20E9DH10C12
- Light chain type
- kappa
- Concentration
- Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product was previously marketed under the MitoSciences sub-brand.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NDUFB8 protein also known as NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 is an integral component of the mitochondrial inner membrane. It is a part of the electron transport chain and plays a role in mitochondrial oxidative phosphorylation. NDUFB8 has a molecular weight of approximately 19 kDa. It is broadly expressed in tissues with high-energy demands like the heart brain skeletal muscle and liver. This expression indicates its significant involvement in energy metabolism.
- Biological function summary
NDUFB8 is an accessory subunit of the mitochondrial Complex I the largest enzyme of the respiratory chain. It assists in the transfer of electrons from NADH to ubiquinone a critical step in cellular respiration that contributes to ATP production. Though not directly involved in catalysis its presence is essential for the structural integrity and assembly of Complex I. By maintaining this complex's structure NDUFB8 helps ensure efficient energy production in cells.
- Pathways
NDUFB8 participates in the oxidative phosphorylation pathway playing a significant role in the mitochondrial respiratory chain. It influences the production of ATP the primary energy carrier in cells. Its association with Complex I links it to other subunits and proteins like NDUFA9 and NDUFA1 in the same pathway. These connections emphasize NDUFB8's importance within the pathway and its contribution to cellular energy management.
- Associated diseases and disorders
Dysfunction in NDUFB8 is linked to mitochondrial disorders often resulting in neurological diseases or muscle weakness such as Leigh syndrome. Mutations affecting NDUFB8 or its associated proteins like NDUFA13 could disrupt the assembly or function of Complex I leading to impaired energy production. This connection highlights the role of NDUFB8 in maintaining cellular energy balance and its impact on health when malfunctioning.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
![Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242), expandable thumbnail](https://content.abcam.com/products/images/ndufb8-antibody-20e9dh10c12-ab110242--immunohistochemistry-frozen-sections-img82229.jpg "Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)")
Wedding, I.M. et al PLoS One. 2014 Jan 22;9(1):e86340. doi: 10.1371/journal.pone.0086340. eCollection 2014. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
Immunohistochemistry in serial sections of the muscle of patient AIV-5The patient is sufferening from Spastic paraplegia 7.
Immunohistochemistry for complex I (NDUFB8) (A), complex II (B), complex III (C) and COX/SDH histochemistry (D) in serial sections of the muscle of patient AIV-5. There are complex I, III and IV deficient fibres, but complex I deficiency is most pronounced. Arrows mark serial sections of the same muscle fibers stained for different complexes.
NDUFB8 (also refered to as complex I) was detected using ab110242 at 1/100 dilution).
(After Figure 3 of Wedding et al)
![Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242), expandable thumbnail](https://content.abcam.com/products/images/ndufb8-antibody-20e9dh10c12-ab110242--western-blot-img36695.jpg "Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)")
Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
All lanes: Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) at 0.5 µg/mL
Lane 1: Isolated mitochondria from Human heart at 5 µg
Lane 2: Isolated mitochondria from cow heart at 1 µg
Lane 3: Isolated mitochondria from rat heart at 10 µg
Lane 4: Isolated mitochondria from mouse heart at 10 µg
Predicted band size: 22 kDa
![Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242), expandable thumbnail](https://content.abcam.com/products/images/ndufb8-antibody-20e9dh10c12-ab110242--immunohistochemistry-frozen-sections-img125852.gif "Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)")
Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242)
Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.
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