Anti-NDUFB8 antibody [20E9DH10C12]

• WB

Unknown

Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (AB110242)

Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

All lanes:

Western blot - Anti-NDUFB8 antibody [20E9DH10C12] (ab110242) at 0.5 µg/mL

Lane 1:

Isolated mitochondria from Human heart at 5 µg

Lane 2:

Isolated mitochondria from cow heart at 1 µg

Lane 3:

Isolated mitochondria from rat heart at 10 µg

Lane 4:

Isolated mitochondria from mouse heart at 10 µg

Predicted band size: 22 kDa

false

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (AB110242)

Skeletal muscle immunohistochemistry using ab110242 on frozen tissue sections from a patient with a single large deletion of the mtDNA show a mosaic of complex I positive and complex I negative fibers.

• IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Anti-NDUFB8 antibody [20E9DH10C12] (AB110242)

Immunohistochemistry in serial sections of the muscle of patient AIV-5

The patient is sufferening from Spastic paraplegia 7.

Immunohistochemistry for complex I (NDUFB8) (A), complex II (B), complex III (C) and COX/SDH histochemistry (D) in serial sections of the muscle of patient AIV-5. There are complex I, III and IV deficient fibres, but complex I deficiency is most pronounced. Arrows mark serial sections of the same muscle fibers stained for different complexes.

NDUFB8 (also refered to as complex I) was detected using ab110242 at 1/100 dilution).

(After Figure 3 of Wedding et al)

Wedding, I.M. et al PLoS One. 2014 Jan 22;9(1):e86340. doi: 10.1371/journal.pone.0086340. eCollection 2014. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/