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Rabbit Recombinant Monoclonal NDUFB8 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples. Cited in 16 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/20

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000 - 1/10000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternative names

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Rabbit Recombinant Monoclonal NDUFB8 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples. Cited in 16 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR15961

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

NDUFB8 is an accessory subunit of the mitochondrial Complex I the largest enzyme of the respiratory chain. It assists in the transfer of electrons from NADH to ubiquinone a critical step in cellular respiration that contributes to ATP production. Though not directly involved in catalysis its presence is essential for the structural integrity and assembly of Complex I. By maintaining this complex's structure NDUFB8 helps ensure efficient energy production in cells.

Activity summary

The NDUFB8 protein also known as NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 is an integral component of the mitochondrial inner membrane. It is a part of the electron transport chain and plays a role in mitochondrial oxidative phosphorylation. NDUFB8 has a molecular weight of approximately 19 kDa. It is broadly expressed in tissues with high-energy demands like the heart brain skeletal muscle and liver. This expression indicates its significant involvement in energy metabolism.

Pathways

NDUFB8 participates in the oxidative phosphorylation pathway playing a significant role in the mitochondrial respiratory chain. It influences the production of ATP the primary energy carrier in cells. Its association with Complex I links it to other subunits and proteins like NDUFA9 and NDUFA1 in the same pathway. These connections emphasize NDUFB8's importance within the pathway and its contribution to cellular energy management.

Associated diseases and disorders

Dysfunction in NDUFB8 is linked to mitochondrial disorders often resulting in neurological diseases or muscle weakness such as Leigh syndrome. Mutations affecting NDUFB8 or its associated proteins like NDUFA13 could disrupt the assembly or function of Complex I leading to impaired energy production. This connection highlights the role of NDUFB8 in maintaining cellular energy balance and its impact on health when malfunctioning.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-NDUFB8 antibody [EPR15961] (ab192878), expandable thumbnail

    Western blot - Anti-NDUFB8 antibody [EPR15961] (ab192878)

    Lanes 1 - 2: Merged signal (red and green). Green - ab192878 observed at 19 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab192878 was shown to specifically react with NDUFB8 in wild-type HAP1 cells as signal was lost in NDUFB8 knockout cells. Wild-type and NDUFB8 knockout samples were subjected to SDS-PAGE. Ab192878 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-NDUFB8 antibody [EPR15961] (AB192878) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: NDUFB8 knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 22 kDa

    Observed band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] (ab192878), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] (ab192878)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-NDUFB8 antibody [EPR15961] (ab192878), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NDUFB8 antibody [EPR15961] (ab192878)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling NDUFB8 antibody (red) with purified ab192878 at a dilution of 1/30. Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Western blot - Anti-NDUFB8 antibody [EPR15961] (ab192878), expandable thumbnail

    Western blot - Anti-NDUFB8 antibody [EPR15961] (ab192878)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-NDUFB8 antibody [EPR15961] (AB192878) at 1/5000 dilution

    Lane 1: Human fetal liver lysate at 20 µg

    Lane 2: Human tonsil lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 19 kDa

  • Western blot - Anti-NDUFB8 antibody [EPR15961] (ab192878), expandable thumbnail

    Western blot - Anti-NDUFB8 antibody [EPR15961] (ab192878)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-NDUFB8 antibody [EPR15961] (AB192878) at 1/20000 dilution

    All lanes: Human fetal heart lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 19 kDa

  • Immunoprecipitation - Anti-NDUFB8 antibody [EPR15961] (ab192878), expandable thumbnail

    Immunoprecipitation - Anti-NDUFB8 antibody [EPR15961] (ab192878)

    Western blot analysis of NDUFB8 immunoprecipitated from Human fetal heart lysate using ab192878 at 1/20 dilution. Lane 1: Human fetal liver lysate. Lane 2: PBS instead of Human fetal liver lysate.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-NDUFB8 antibody [EPR15961] (AB192878)

    Predicted band size: 22 kDa

  • Western blot - Anti-NDUFB8 antibody [EPR15961] (ab192878), expandable thumbnail

    Western blot - Anti-NDUFB8 antibody [EPR15961] (ab192878)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-NDUFB8 antibody [EPR15961] (AB192878) at 1/5000 dilution

    Lane 1: C6 cell lysate at 10 µg

    Lane 2: RAW 264.7 cell lysate at 10 µg

    Lane 3: PC-12 cell lysate at 10 µg

    Lane 4: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa

    Observed band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] (ab192878), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] (ab192878)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] (ab192878), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] (ab192878)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFB8 antibody [EPR15961] (ab192878), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NDUFB8 antibody [EPR15961] (ab192878)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NDUFB8 with ab192878 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary antibody at 1/400 dilution. Nuclear counter stained is DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] (ab192878), expandable thumbnail
    This image is courtesy of a customer review submitted by Diego Perez Rodriguez

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] (ab192878)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed mouse skeletal muscle tissue permeabilized with 0.3% Triton X100 in PBS stained with ab192878 at 1/50 dilution. Secondary antibody was Goat Anti-Rabbit IgG Antibody (H+L), Biotinylated at 1/3000 dilution. Samples were incubated with the primary antibody with 5% goat serum in 0.2% Triton X100 in PBS for 16 hours at 4°C. Blocking was done using 5% serum for 1 hour at 21°C. Heat mediated antigen retrieval with Tris/EDTA pH 9.0. ABC system used for signal amplification. Chromogenic reaction developed with DAB Substrate Kit (ab64238).Cell nuclei counterstained with Gill's hematoxylin I.

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Product protocols

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