Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (AB251160)

This data was developed using ab192878, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling NDUFB8 antibody (red) with purified ab192878 at a dilution of 1/30. Goat anti rabbit IgG (Alexa Fluor^® 488) was used as the secondary antibody at 1/2000. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (AB251160)

This data was developed using ab192878, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (AB251160)

This data was developed using ab192878, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NDUFB8 with ab192878 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary antibody at 1/400 dilution. Nuclear counter stained is DAPI (blue).

• IP

Supplier Data

Immunoprecipitation - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (AB251160)

This data was developed using ab192878, the same antibody clone in a different buffer formulation.

Western blot analysis of NDUFB8 immunoprecipitated from Human fetal heart lysate using ab192878 at 1/20 dilution. Lane 1 : Human fetal liver lysate. Lane 2 : PBS instead of Human fetal liver lysate.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-NDUFB8 antibody [EPR15961] (<a href='/en-us/products/primary-antibodies/ndufb8-antibody-epr15961-ab192878'>ab192878</a>)

Predicted band size: 22 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (AB251160)

This data was developed using ab192878, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (AB251160)

This data was developed using ab192878, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling NDUFB8 with ab192878 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (AB251160)

This data was developed using ab192878, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NDUFB8 antibody [EPR15961] (<a href='/en-us/products/primary-antibodies/ndufb8-antibody-epr15961-ab192878'>ab192878</a>) at 1/20000 dilution

All lanes:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 19 kDa

false

• WB

Supplier Data

Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (AB251160)

This data was developed using ab192878, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NDUFB8 antibody [EPR15961] (<a href='/en-us/products/primary-antibodies/ndufb8-antibody-epr15961-ab192878'>ab192878</a>) at 1/5000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human tonsil lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 19 kDa

false

• WB

Lab

Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (AB251160)

This data was developed using ab192878, the same antibody clone in a different buffer formulation.

Lanes 1 - 2 Merged signal (red and green). Green - ab192878 observed at 19 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab192878 was shown to specifically react with NDUFB8 in wild-type HAP1 cells as signal was lost in NDUFB8 knockout cells. Wild-type and NDUFB8 knockout samples were subjected to SDS-PAGE. ab192878 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NDUFB8 antibody [EPR15961] (<a href='/en-us/products/primary-antibodies/ndufb8-antibody-epr15961-ab192878'>ab192878</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

NDUFB8 knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 22 kDa

Observed band size: 19 kDa

false

• WB

Supplier Data

Western blot - Anti-NDUFB8 antibody [EPR15961] - BSA and Azide free (AB251160)

This data was developed using ab192878, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NDUFB8 antibody [EPR15961] (<a href='/en-us/products/primary-antibodies/ndufb8-antibody-epr15961-ab192878'>ab192878</a>) at 1/5000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 19 kDa

false