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Rabbit Recombinant Monoclonal NDUFB9 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

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Images

Western blot - Anti-NDUFB9 antibody [EPR15955-78] (AB200198), expandable thumbnail
  • Western blot - Anti-NDUFB9 antibody [EPR15955-78] (AB200198), expandable thumbnail
  • Western blot - Anti-NDUFB9 antibody [EPR15955-78] (AB200198), expandable thumbnail
  • Western blot - Anti-NDUFB9 antibody [EPR15955-78] (AB200198), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955-78] (AB200198), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/150
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternative names

LYRM3, UQOR22, NDUFB9, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9, Complex I-B22, LYR motif-containing protein 3, NADH-ubiquinone oxidoreductase B22 subunit, CI-B22

Recommended products

Rabbit Recombinant Monoclonal NDUFB9 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15955-78
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein NDUFB9 also known as NADH:ubiquinone oxidoreductase subunit B9 is a part of the mitochondrial respiratory chain. This protein has a molecular mass of approximately 22 kDa. NDUFB9 is expressed in the mitochondria of many tissues serving as an accessory subunit of complex I the first enzyme in the electron transport chain. It does not participate in catalysis but assists in maintaining the integrity and stability of the complex.

Biological function summary

NDUFB9 plays a role in mitochondrial energy production. As a component of complex I which is the largest of the five mitochondrial complexes NDUFB9 contributes to the process of oxidative phosphorylation. This complex transfers electrons from NADH to ubiquinone facilitating the pumping of protons across the inner mitochondrial membrane. This proton gradient drives ATP production vital for energy metabolism in cells.

Pathways

The function of NDUFB9 is critical in cellular respiration and energy production pathways. It is integral to the pathway of oxidative phosphorylation closely associated with aerobic energy metabolism. Through its role in complex I NDUFB9 interacts with other mitochondrial proteins such as NDUFA1 and NDUFS1 which are also part of the same complex and contribute to electron transport and energy conservation.

Associated diseases and disorders

NDUFB9 mutations have been linked to mitochondrial disorders including Leigh syndrome a severe neurological disorder characterized by progressive loss of mental and movement abilities. NDUFB9 dysfunction can also contribute to Complex I deficiency which leads to a range of metabolic syndromes. This protein's interaction with other complex I proteins like NDUFS2 is important for maintaining proper mitochondrial function and disturbances can lead to broader mitochondrial pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198), expandable thumbnail

    Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)

    Lanes 1-3: Merged signal (red and green). Green - ab200198 observed at 22 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab200198 Anti-NDUFB9 antibody [EPR15955-78] was shown to specifically react with NDUFB9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human NDUFB9 knockout HeLa cell line ab265946 (knockout cell lysate Human NDUFB9 knockout HeLa cell lysate ab258065) was used. Wild-type and NDUFB9 knockout samples were subjected to SDS-PAGE. ab200198 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: NDUFB9 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human NDUFB9 knockout HeLa cell line (Human NDUFB9 knockout HeLa cell line ab265946)

    Lane 3: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 21 kDa

    Observed band size: 22 kDa

  • Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198), expandable thumbnail

    Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/5000 dilution

    Lane 1: Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µg

    Lane 2: HEK293 (Human embryonic kidney) cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa

    Observed band size: 22 kDa

    Exposure time: 3min

  • Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198), expandable thumbnail

    Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/5000 dilution

    All lanes: Human fetal liver lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 21 kDa

    Observed band size: 22 kDa

    Exposure time: 1min

  • Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198), expandable thumbnail

    Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-NDUFB9 antibody [EPR15955-78] (ab200198) at 1/1000 dilution

    Lane 1: Mouse kidney lysate at 10 µg

    Lane 2: Mouse spleen lysate at 10 µg

    Lane 3: Rat heart lysate at 10 µg

    Lane 4: Rat spleen lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 21 kDa

    Observed band size: 22 kDa

    Exposure time: 1min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955-78] (ab200198), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.

    Cytoplasm staining on Human kidney tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955-78] (ab200198), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.

    Cytoplasm staining on mouse cardiac muscle tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-NDUFB9 antibody [EPR15955-78] (ab200198), expandable thumbnail

    Immunoprecipitation - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)

    NDUFB9 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200198 at 1/150 dilution.

    Western blot was performed from the immunoprecipitate using ab200198 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Jurkat whole cell lysate 10 μg (Input).

    Lane 2: ab200198 IP in Jurkat whole cell elysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab200198 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)

    Predicted band size: 21 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFB9 antibody [EPR15955-78] (ab200198), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NDUFB9 with ab200198 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).

    Cytoplasm staining on Jurkat cell line is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab200198 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow Cytometry (Intracellular) - Anti-NDUFB9 antibody [EPR15955-78] (ab200198), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NDUFB9 antibody [EPR15955-78] (ab200198)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NDUFB9 with ab200198 at 1/400 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730;black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

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Product protocols

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