Skip to main content

Rabbit Recombinant Monoclonal NDUFC2 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Western blot - Anti-NDUFC2 antibody [EPR16499] (AB192265), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFC2 antibody [EPR16499] (AB192265), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-NDUFC2 antibody [EPR16499] (AB192265), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/30

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternative names

NADH dehydrogenase [ubiquinone] 1 subunit C2, Complex I-B14.5b, Human lung cancer oncogene 1 protein, NADH-ubiquinone oxidoreductase subunit B14.5b, CI-B14.5b, HLC-1, NDUFC2, HLC1

Recommended products

Rabbit Recombinant Monoclonal NDUFC2 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

Alternative names

NADH dehydrogenase [ubiquinone] 1 subunit C2, Complex I-B14.5b, Human lung cancer oncogene 1 protein, NADH-ubiquinone oxidoreductase subunit B14.5b, CI-B14.5b, HLC-1, NDUFC2, HLC1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR16499

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-NDUFC2 antibody [EPR16499] (ab192265), expandable thumbnail

    Western blot - Anti-NDUFC2 antibody [EPR16499] (ab192265)

    Blocking/Dilution buffer: 5% NFDM /TBST.

    All lanes: Western blot - Anti-NDUFC2 antibody [EPR16499] (ab192265) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: HepG2 cell lysate at 20 µg

    Lane 3: A549 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa

    Observed band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFC2 antibody [EPR16499] (ab192265), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFC2 antibody [EPR16499] (ab192265)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFC2 with ab192265 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-NDUFC2 antibody [EPR16499] (ab192265), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NDUFC2 antibody [EPR16499] (ab192265)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed A549 cells labeling NDUFC2 with ab192265 at 1/30 dilution (pink) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com