AB185733

Anti-Ndufs1 antibody

Name: Anti-Ndufs1 antibody (ab185733) | Abcam
Brand: Abcam Limited
SKU: AB185733
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Ndufs1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NDUFS1 aa 50-300.

View Alternative Names

Complex I-75kD, CI-75kD, NDUFS1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody (AB185733)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody (AB185733)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Ndufs1 with ab185733 at 1/200. Magnification : 400x.

Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody (AB185733)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody (AB185733)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185733. Blue DAPI for nuclear staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody (AB185733)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Ndufs1 with ab185733 at 1/200. Magnification : 400x.

Supplier Data

Western blot - Anti-Ndufs1 antibody (AB185733)

All lanes:

Western blot - Anti-Ndufs1 antibody (ab185733) at 1/500 dilution

Lane 1:

HeLa cell line extract

Lane 2:

Raji cell line extract

Lane 3:

H460 cell line extract

Lane 4:

PC3 cell line extract

Lane 5:

293T cell line extract

Lane 6:

HepG2 cell line extract

Lane 7:

Mouse kidney tissue extract

Lane 8:

Mouse heart tissue extract

Lane 9:

Mouse intestine tissue extract

Predicted band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NDUFS1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

P28331

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ndufs1 known as NADH:ubiquinone oxidoreductase core subunit S1 or complex I 75 kDa subunit forms a significant part of the mitochondrial electron transport chain. This protein shows a mass of 75 kDa and is primarily found in mitochondria among various tissues. Ndufs1 plays an important role in transferring electrons from NADH to ubiquinone facilitating proton translocation across the inner mitochondrial membrane. With its function it contributes to the creation of a proton gradient essential for ATP synthesis.

Biological function summary

Ndufs1 facilitates the primary catalytic activity of the NADH dehydrogenase complex also found within the mitochondrial complex I. By being part of this large multi-subunit complex it helps in electron flow which essential for cellular respiration. Proper functioning of Ndufs1 becomes necessary for maintaining adequate energy production through ATP synthesis in cells impacting energy-dependent processes significantly.

Pathways

Ndufs1 acts mainly within the oxidative phosphorylation pathway. This pathway is indispensable for energy conversion in cells prominently featuring NADH dehydrogenase (ubiquinone). It relates closely with other critical proteins like Ndufa9 and Ndufb8 as these proteins also integrate into the mitochondrial complex I ensuring effective electron transport and energy yield in cells. The pathway links metabolic flux to energy production keeping cellular operations efficient.

Ndufs1 mutations correlate with mitochondrial diseases such as Leigh syndrome and mitochondrial complex I deficiency. These conditions often manifest neurological and muscular symptoms due to impaired mitochondrial function. Ndufs1 connections with proteins like Ndufv1 and additional complex I subunits emphasize its role in regular mitochondrial activity making its study relevant for understanding the pathological basis of these disorders. Researchers focus on Ndufs1 to unravel its impact on cellular metabolism and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 30879903, PubMed : 31557978). Essential for catalysing the entry and efficient transfer of electrons within complex I (PubMed : 31557978). Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes (PubMed : 30879903, PubMed : 31557978).

See full target information NDUFS1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37685993

2023

Novel Function of Nogo-A as Negative Regulator of Endothelial Progenitor Cell Angiogenic Activity: Impact in Oxygen-Induced Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Pakiza Ruknudin,Ali Riza Nazari,Maelle Wirth,Isabelle Lahaie,Emmanuel Bajon,Alain Rivard,Sylvain Chemtob,Michel Desjarlais

Brain communications 4:fcac102 PubMed35602653

2022

Disruption of cellular iron homeostasis by missense variants causes severe neurodevelopmental delay, dystonia and seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Nunziata Maio,Russell P Saneto,Richard Steet,Marcio A Sotero de Menezes,Cindy Skinner,Tracey A Rouault

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Ndufs1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody (AB185733)

Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody (AB185733)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody (AB185733)

Western blot - Anti-Ndufs1 antibody (AB185733)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise