AB96428

Anti-Ndufs1 antibody

Name: Anti-Ndufs1 antibody (ab96428) | Abcam
Brand: Abcam Limited
SKU: AB96428
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Ndufs1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NDUFS1 aa 1-200.

View Alternative Names

Complex I-75kD, CI-75kD, NDUFS1

1 Images

Supplier Data

Western blot - Anti-Ndufs1 antibody (AB96428)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Ndufs1 antibody (ab96428) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysates at 50 µg

Lane 2:

Rat brain tissue lysates at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human NDUFS1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P28331

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ndufs1 known as NADH:ubiquinone oxidoreductase core subunit S1 or complex I 75 kDa subunit forms a significant part of the mitochondrial electron transport chain. This protein shows a mass of 75 kDa and is primarily found in mitochondria among various tissues. Ndufs1 plays an important role in transferring electrons from NADH to ubiquinone facilitating proton translocation across the inner mitochondrial membrane. With its function it contributes to the creation of a proton gradient essential for ATP synthesis.

Biological function summary

Ndufs1 facilitates the primary catalytic activity of the NADH dehydrogenase complex also found within the mitochondrial complex I. By being part of this large multi-subunit complex it helps in electron flow which essential for cellular respiration. Proper functioning of Ndufs1 becomes necessary for maintaining adequate energy production through ATP synthesis in cells impacting energy-dependent processes significantly.

Pathways

Ndufs1 acts mainly within the oxidative phosphorylation pathway. This pathway is indispensable for energy conversion in cells prominently featuring NADH dehydrogenase (ubiquinone). It relates closely with other critical proteins like Ndufa9 and Ndufb8 as these proteins also integrate into the mitochondrial complex I ensuring effective electron transport and energy yield in cells. The pathway links metabolic flux to energy production keeping cellular operations efficient.

Ndufs1 mutations correlate with mitochondrial diseases such as Leigh syndrome and mitochondrial complex I deficiency. These conditions often manifest neurological and muscular symptoms due to impaired mitochondrial function. Ndufs1 connections with proteins like Ndufv1 and additional complex I subunits emphasize its role in regular mitochondrial activity making its study relevant for understanding the pathological basis of these disorders. Researchers focus on Ndufs1 to unravel its impact on cellular metabolism and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 30879903, PubMed : 31557978). Essential for catalysing the entry and efficient transfer of electrons within complex I (PubMed : 31557978). Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes (PubMed : 30879903, PubMed : 31557978).

See full target information NDUFS1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 22:862-74 PubMed25361078

2014

Granzyme B-induced mitochondrial ROS are required for apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

G Jacquemin,D Margiotta,A Kasahara,E Y Bassoy,M Walch,J Thiery,J Lieberman,D Martinvalet

PloS one 9:e106779 PubMed25215595

2014

Profiling the mitochondrial proteome of Leber's Hereditary Optic Neuropathy (LHON) in Thailand: down-regulation of bioenergetics and mitochondrial protein quality control pathways in fibroblasts with the 11778G>A mutation.

Applications

Species

Human

Aung Win Tun,Sakdithep Chaiyarit,Supannee Kaewsutthi,Wanphen Katanyoo,Wanicha Chuenkongkaew,Masayoshi Kuwano,Takeshi Tomonaga,Chayanon Peerapittayamongkol,Visith Thongboonkerd,Patcharee Lertrit

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Ndufs1 antibody

Western blot - Anti-Ndufs1 antibody (AB96428)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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