AB157221

Anti-Ndufs1 antibody [EPR11522(B)]

RabMAb
Recombinant
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(10 Publications)

Rabbit Recombinant Monoclonal Ndufs1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 10 publications.

View Alternative Names

Complex I-75kD, CI-75kD, NDUFS1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

ab157221 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

ab157221 showing +ve staining in Human cervical carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Ndufs1 with ab157221 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

ab157221 showing +ve staining in Human heart.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ndufs1 with ab157221 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab157221 at 1/50 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

ab157221 showing +ve staining in Human normal prostate.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ndufs1 with ab157221 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

All lanes:

Western blot - Anti-Ndufs1 antibody [EPR11522(B)] (ab157221) at 1/10000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

MOLT4 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 79 kDa

false

Carrier free

Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11522(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ndufs1 known as NADH:ubiquinone oxidoreductase core subunit S1 or complex I 75 kDa subunit forms a significant part of the mitochondrial electron transport chain. This protein shows a mass of 75 kDa and is primarily found in mitochondria among various tissues. Ndufs1 plays an important role in transferring electrons from NADH to ubiquinone facilitating proton translocation across the inner mitochondrial membrane. With its function it contributes to the creation of a proton gradient essential for ATP synthesis.

Biological function summary

Ndufs1 facilitates the primary catalytic activity of the NADH dehydrogenase complex also found within the mitochondrial complex I. By being part of this large multi-subunit complex it helps in electron flow which essential for cellular respiration. Proper functioning of Ndufs1 becomes necessary for maintaining adequate energy production through ATP synthesis in cells impacting energy-dependent processes significantly.

Pathways

Ndufs1 acts mainly within the oxidative phosphorylation pathway. This pathway is indispensable for energy conversion in cells prominently featuring NADH dehydrogenase (ubiquinone). It relates closely with other critical proteins like Ndufa9 and Ndufb8 as these proteins also integrate into the mitochondrial complex I ensuring effective electron transport and energy yield in cells. The pathway links metabolic flux to energy production keeping cellular operations efficient.

Ndufs1 mutations correlate with mitochondrial diseases such as Leigh syndrome and mitochondrial complex I deficiency. These conditions often manifest neurological and muscular symptoms due to impaired mitochondrial function. Ndufs1 connections with proteins like Ndufv1 and additional complex I subunits emphasize its role in regular mitochondrial activity making its study relevant for understanding the pathological basis of these disorders. Researchers focus on Ndufs1 to unravel its impact on cellular metabolism and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 30879903, PubMed : 31557978). Essential for catalysing the entry and efficient transfer of electrons within complex I (PubMed : 31557978). Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes (PubMed : 30879903, PubMed : 31557978).

See full target information NDUFS1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature 642:492-500 PubMed40335696

2025

Activation of lysosomal iron triggers ferroptosis in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Cañeque,Leeroy Baron,Sebastian Müller,Alanis Carmona,Ludovic Colombeau,Antoine Versini,Stéphanie Solier,Christine Gaillet,Fabien Sindikubwabo,Julio L Sampaio,Marie Sabatier,Eikan Mishima,Armel Picard-Bernes,Laurène Syx,Nicolas Servant,Bérangère Lombard,Damarys Loew,Jiashuo Zheng,Bettina Proneth,Leishemba K Thoidingjam,Laurence Grimaud,Cameron S Fraser,Krystina J Szylo,Emma Der Kazarian,Caroline Bonnet,Emmanuelle Charafe-Jauffret,Christophe Ginestier,Patricia Santofimia-Castaño,Matias Estaras,Nelson Dusetti,Juan Lucio Iovanna,Antonio Sa Cunha,Gabriella Pittau,Pascal Hammel,Dimitri Tzanis,Sylvie Bonvalot,Sarah Watson,Vincent Gandon,Aditya Upadhyay,Derek A Pratt,Florêncio Porto Freitas,José Pedro Friedmann Angeli,Brent R Stockwell,Marcus Conrad,Jessalyn M Ubellacker,Raphaël Rodriguez

Neurobiology of disease 194:106474 PubMed38518837

2024

Engrailed 1 deficiency induces changes in ciliogenesis during human neuronal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Sina Hembach,Sebastian Schmidt,Tanja Orschmann,Ingo Burtscher,Heiko Lickert,Florian Giesert,Daniela Vogt Weisenhorn,Wolfgang Wurst

British journal of cancer 129:1261-1273 PubMed37644092

2023

Loss of NDUFS1 promotes gastric cancer progression by activating the mitochondrial ROS-HIF1α-FBLN5 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Chen,Dongbao Li,Yunliang Wang,Xiaochun Shen,Anqi Dong,Chao Dong,Kaipeng Duan,Jiayu Ren,Weikang Li,Gege Shu,Jiaoyang Yang,Yufeng Xie,Fuliang Qian,Jin Zhou

Alcoholism, clinical and experimental research 46:556-569 PubMed35187673

2022

Gag-like proteins: Novel mediators of prenatal alcohol exposure in neural development.

Applications

Unspecified application

Species

Unspecified reactive species

Marisa R Pinson,Dae D Chung,Amanda H Mahnke,Nihal A Salem,Daniel Osorio,Vijay Nair,Elizabeth A Payne,Jonathan J Del Real,James J Cai,Rajesh C Miranda

Journal of lipid research 63:100173 PubMed35101424

2022

KIAA1363 affects retinyl ester turnover in cultured murine and human hepatic stellate cells.

Applications

Unspecified application

Species

Unspecified reactive species

Carina Wagner,Victoria Hois,Annalena Eggeling,Lisa-Maria Pusch,Laura Pajed,Patrick Starlinger,Thierry Claudel,Michael Trauner,Robert Zimmermann,Ulrike Taschler,Achim Lass

Cell death & disease 12:839 PubMed34497268

2021

CISD3 inhibition drives cystine-deprivation induced ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchun Li,Xin Wang,Zhihui Huang,Yi Zhou,Jun Xia,Wanye Hu,Xu Wang,Jing Du,Xiangmin Tong,Ying Wang

Immunity 54:53-67.e7 PubMed33058782

2020

Tetracycline Antibiotics Induce Host-Dependent Disease Tolerance to Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Henrique G Colaço,André Barros,Ana Neves-Costa,Elsa Seixas,Dora Pedroso,Tiago Velho,Katharina L Willmann,Pedro Faisca,Gerlinde Grabmann,Hyon-Seung Yi,Minho Shong,Vladimir Benes,Sebastian Weis,Thomas Köcher,Luís F Moita

Asian journal of andrology 22:88-93 PubMed31006710

2019

Reduced semen quality in patients with testicular cancer seminoma is associated with alterations in the expression of sperm proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Tânia R Dias,Ashok Agarwal,Peter N Pushparaj,Gulfam Ahmad,Rakesh Sharma

Nature communications 8:14859 PubMed28327588

2017

Pharmacological inhibition of adipose triglyceride lipase corrects high-fat diet-induced insulin resistance and hepatosteatosis in mice.

Applications

Species

Unspecified reactive species

Martina Schweiger,Matthias Romauch,Renate Schreiber,Gernot F Grabner,Sabrina Hütter,Petra Kotzbeck,Pia Benedikt,Thomas O Eichmann,Sohsuke Yamada,Oskar Knittelfelder,Clemens Diwoky,Carina Doler,Nicole Mayer,Werner De Cecco,Rolf Breinbauer,Robert Zimmermann,Rudolf Zechner

Cell metabolism 20:433-47 PubMed25043816

2014

A switch from white to brown fat increases energy expenditure in cancer-associated cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Michele Petruzzelli,Martina Schweiger,Renate Schreiber,Ramon Campos-Olivas,Maria Tsoli,John Allen,Michael Swarbrick,Stefan Rose-John,Mercedes Rincon,Graham Robertson,Rudolf Zechner,Erwin F Wagner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ndufs1 antibody [EPR11522(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Flow Cytometry (Intracellular) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

Western blot - Anti-Ndufs1 antibody [EPR11522(B)] (AB157221)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise