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AB249321

Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Ndufs1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Complex I-75kD, CI-75kD, NDUFS1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.

ab157221 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.

ab157221 showing +ve staining in Human cervical carcinoma.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Ndufs1 with ab157221 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.

ab157221 showing +ve staining in Human heart.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ndufs1 with ab157221 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab157221 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.

ab157221 showing +ve staining in Human normal prostate.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ndufs1 with ab157221 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)
  • WB

Unknown

Western blot - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (AB249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Ndufs1 antibody [EPR11522(B)] (<a href='/en-us/products/primary-antibodies/ndufs1-antibody-epr11522b-ab157221'>ab157221</a>) at 1/10000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

MOLT4 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11522(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249321 is the carrier-free version of ab157221.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ndufs1 known as NADH:ubiquinone oxidoreductase core subunit S1 or complex I 75 kDa subunit forms a significant part of the mitochondrial electron transport chain. This protein shows a mass of 75 kDa and is primarily found in mitochondria among various tissues. Ndufs1 plays an important role in transferring electrons from NADH to ubiquinone facilitating proton translocation across the inner mitochondrial membrane. With its function it contributes to the creation of a proton gradient essential for ATP synthesis.
Biological function summary

Ndufs1 facilitates the primary catalytic activity of the NADH dehydrogenase complex also found within the mitochondrial complex I. By being part of this large multi-subunit complex it helps in electron flow which essential for cellular respiration. Proper functioning of Ndufs1 becomes necessary for maintaining adequate energy production through ATP synthesis in cells impacting energy-dependent processes significantly.

Pathways

Ndufs1 acts mainly within the oxidative phosphorylation pathway. This pathway is indispensable for energy conversion in cells prominently featuring NADH dehydrogenase (ubiquinone). It relates closely with other critical proteins like Ndufa9 and Ndufb8 as these proteins also integrate into the mitochondrial complex I ensuring effective electron transport and energy yield in cells. The pathway links metabolic flux to energy production keeping cellular operations efficient.

Ndufs1 mutations correlate with mitochondrial diseases such as Leigh syndrome and mitochondrial complex I deficiency. These conditions often manifest neurological and muscular symptoms due to impaired mitochondrial function. Ndufs1 connections with proteins like Ndufv1 and additional complex I subunits emphasize its role in regular mitochondrial activity making its study relevant for understanding the pathological basis of these disorders. Researchers focus on Ndufs1 to unravel its impact on cellular metabolism and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 30879903, PubMed : 31557978). Essential for catalysing the entry and efficient transfer of electrons within complex I (PubMed : 31557978). Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes (PubMed : 30879903, PubMed : 31557978).
See full target information NDUFS1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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