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AB192764

Anti-NDUFS2 antibody

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(1 Publication)

Rabbit Polyclonal NDUFS2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial aa 200-450.

View Alternative Names

Complex I-49kD, NADH-ubiquinone oxidoreductase 49 kDa subunit, CI-49kD, NDUFS2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody (AB192764)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody (AB192764)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling NDUFS2 with ab192764 at 7.5μg/mL.

Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody (AB192764)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody (AB192764)

Immunofluorescent analysis of HeLa cells labeling mitochondrial NDUFS2 with ab192764 at a 1/500 dilution. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37 for 30 min. The panel on the right shows the nuclear stain.

Western blot - Anti-NDUFS2 antibody (AB192764)
  • WB

Supplier Data

Western blot - Anti-NDUFS2 antibody (AB192764)

All lanes:

Western blot - Anti-NDUFS2 antibody (ab192764) at 1/1000 dilution

Lane 1:

293T cell lysate at 30 µg

Lane 2:

A431 cell lysate at 30 µg

Lane 3:

Jurkat cell lysate at 30 µg

Lane 4:

Raji cell lysate at 30 µg

Predicted band size: 53 kDa

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Western blot - Anti-NDUFS2 antibody (AB192764)
  • WB

Supplier Data

Western blot - Anti-NDUFS2 antibody (AB192764)

All lanes:

Western blot - Anti-NDUFS2 antibody (ab192764) at 1/10000 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

Predicted band size: 53 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial aa 200-450. The exact immunogen used to generate this antibody is proprietary information.

O75306

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "7.5 µg/mL", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 79% Tris glycine, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFS2 also known as NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 is a significant component of complex I in the mitochondrial respiratory chain. This protein has a mass of approximately 54 kDa and is primarily found in mitochondrial membranes of cells across various tissues. It plays an essential role in electron transfer working closely with other subunits to facilitate the conversion of NADH to NAD+ contributing to the process of oxidative phosphorylation.
Biological function summary

NDUFS2 serves an important role in energy production as part of the multi-subunit complex I which is also known as NADH:ubiquinone oxidoreductase. This complex catalyzes the first step in the mitochondrial electron transport chain transferring electrons from NADH to ubiquinone. Through this process NDUFS2 helps power ATP synthesis by contributing to the establishment of a proton gradient across the inner mitochondrial membrane which is fundamental to cellular energy metabolism.

Pathways

The function of NDUFS2 is integral to both the oxidative phosphorylation and citric acid cycle pathways. Within oxidative phosphorylation NDUFS2 associates closely with other complex I subunits such as NDUFS1 and NDUFV1. These associations allow it to effectively participate in the electron transport chain facilitating energy production. The citric acid cycle indirectly impacts NDUFS2's function by supplying NADH which is necessary for complex I activity and efficient energy conversion.

NDUFS2 mutations and dysfunctions relate to disorders such as Leigh syndrome and some mitochondrial myopathies. Leigh syndrome a severe neurological disorder often involves deficiencies in complex I where NDUFS2 plays a significant role. This protein also shows connections to other mitochondrial proteins like NDUFA12 and NDUFB6 which when also impaired can contribute to disease manifestations. The study and understanding of NDUFS2's role in these conditions are important for developing potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 22036843, PubMed : 28031252, PubMed : 30922174). Essential for the catalytic activity of complex I (PubMed : 22036843, PubMed : 30922174). Essential for the assembly of complex I (By similarity). Redox-sensitive, critical component of the oxygen-sensing pathway in the pulmonary vasculature which plays a key role in acute pulmonary oxygen-sensing and hypoxic pulmonary vasoconstriction (PubMed : 30922174). Plays an important role in carotid body sensing of hypoxia (By similarity). Essential for glia-like neural stem and progenitor cell proliferation, differentiation and subsequent oligodendrocyte or neuronal maturation (By similarity).
See full target information NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1613438 PubMed40978465

2025

Abnormal Ca handling and reduced I contribute to citalopram-induced QT prolongation and cardiac arrhythmias.

Applications

Unspecified application

Species

Unspecified reactive species

Yangpeng Li,Jiamin Xie,Yuqing Zheng,Jianhong Li,Min Zhao,Li Liu,Hongping Shen,Ming Lei,Xiaoqiu Tan,Juan Xiao,Xueru Liu,Tangting Chen
View all publications
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Product promise

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