JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB216510

Anti-NDUFS2 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal NDUFS2 antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial aa 350-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Complex I-49kD, NADH-ubiquinone oxidoreductase 49 kDa subunit, CI-49kD, NDUFS2

1 Images
Western blot - Anti-NDUFS2 antibody (AB216510)
  • WB

Supplier Data

Western blot - Anti-NDUFS2 antibody (AB216510)

All lanes:

Western blot - Anti-NDUFS2 antibody (ab216510) at 1/300 dilution

All lanes:

Mouse heart lysates

Secondary

All lanes:

Goat anti-rabbit IgG (H+L), HRP conjugated (90 min at 37˚C) at 1/5000 dilution

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O75306

style
left-column

Alternative Products

Primary Antibodies

AB192764

Anti-NDUFS2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody (AB192764)

  • 0
  • 0
Primary Antibodies

AB110249

Anti-NDUFS2 antibody [7A12BE5AD5]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-NDUFS2 antibody [7A12BE5AD5] (AB110249)

  • 5
  • 1
Primary Antibodies

AB192022

Anti-NDUFS2 antibody [EPR16266]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS2 antibody [EPR16266] (AB192022)

  • 0
  • 0

Complementary Products

Assay Kits

AB109721

Complex I Enzyme Activity Microplate Assay Kit (Colorimetric)

Functional Studies - Complex I Enzyme Activity Microplate Assay Kit (Colorimetric) (AB109721)

  • 5
  • 4
Primary Antibodies

AB169540

Anti-Ndufs1 antibody [EPR11521(B)]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (AB169540)

  • 5
  • 2
Primary Antibodies

AB14713

Anti-NDUFA9 antibody [20C11B11B11]

KO Validated

Western blot - Anti-NDUFA9 antibody [20C11B11B11] (AB14713)

  • 4
  • 8
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

Recombinant|Lab Essentials

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
  • 52
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFS2 also known as NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 is a significant component of complex I in the mitochondrial respiratory chain. This protein has a mass of approximately 54 kDa and is primarily found in mitochondrial membranes of cells across various tissues. It plays an essential role in electron transfer working closely with other subunits to facilitate the conversion of NADH to NAD+ contributing to the process of oxidative phosphorylation.
Biological function summary

NDUFS2 serves an important role in energy production as part of the multi-subunit complex I which is also known as NADH:ubiquinone oxidoreductase. This complex catalyzes the first step in the mitochondrial electron transport chain transferring electrons from NADH to ubiquinone. Through this process NDUFS2 helps power ATP synthesis by contributing to the establishment of a proton gradient across the inner mitochondrial membrane which is fundamental to cellular energy metabolism.

Pathways

The function of NDUFS2 is integral to both the oxidative phosphorylation and citric acid cycle pathways. Within oxidative phosphorylation NDUFS2 associates closely with other complex I subunits such as NDUFS1 and NDUFV1. These associations allow it to effectively participate in the electron transport chain facilitating energy production. The citric acid cycle indirectly impacts NDUFS2's function by supplying NADH which is necessary for complex I activity and efficient energy conversion.

NDUFS2 mutations and dysfunctions relate to disorders such as Leigh syndrome and some mitochondrial myopathies. Leigh syndrome a severe neurological disorder often involves deficiencies in complex I where NDUFS2 plays a significant role. This protein also shows connections to other mitochondrial proteins like NDUFA12 and NDUFB6 which when also impaired can contribute to disease manifestations. The study and understanding of NDUFS2's role in these conditions are important for developing potential therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 22036843, PubMed : 28031252, PubMed : 30922174). Essential for the catalytic activity of complex I (PubMed : 22036843, PubMed : 30922174). Essential for the assembly of complex I (By similarity). Redox-sensitive, critical component of the oxygen-sensing pathway in the pulmonary vasculature which plays a key role in acute pulmonary oxygen-sensing and hypoxic pulmonary vasoconstriction (PubMed : 30922174). Plays an important role in carotid body sensing of hypoxia (By similarity). Essential for glia-like neural stem and progenitor cell proliferation, differentiation and subsequent oligodendrocyte or neuronal maturation (By similarity).
See full target information NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadl0030 PubMed38924398

2024

KIBRA anchoring the action of PKMζ maintains the persistence of memory.

Applications

Unspecified application

Species

Unspecified reactive species

Panayiotis Tsokas,Changchi Hsieh,Rafael E Flores-Obando,Matteo Bernabo,Andrew Tcherepanov,A Iván Hernández,Christian Thomas,Peter J Bergold,James E Cottrell,Joachim Kremerskothen,Harel Z Shouval,Karim Nader,André A Fenton,Todd C Sacktor
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com