Mouse Monoclonal NDUFS3 antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse, Cow, Rat samples. Cited in 108 publications.
Images
![Flow Cytometry - Anti-NDUFS3 antibody [17D95] (AB14711), expandable thumbnail](https://content.abcam.com/products/images/ndufs3-antibody-17d95-ab14711--flow-cytometry-img50760.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-NDUFS3 antibody [17D95] (AB14711)")
![Western blot - Anti-NDUFS3 antibody [17D95] (AB14711), expandable thumbnail](https://content.abcam.com/products/images/ndufs3-antibody-17d95-ab14711--western-blot-img80146.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NDUFS3 antibody [17D95] (AB14711)")
![Western blot - Anti-NDUFS3 antibody [17D95] (AB14711), expandable thumbnail](https://content.abcam.com/products/images/ndufs3-antibody-17d95-ab14711--western-blot-img7436.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NDUFS3 antibody [17D95] (AB14711)")
![Western blot - Anti-NDUFS3 antibody [17D95] (AB14711), expandable thumbnail](https://content.abcam.com/products/images/ndufs3-antibody-17d95-ab14711--western-blot-img102336.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NDUFS3 antibody [17D95] (AB14711)")
Publications
Filter by
- Parkinson's disease 2023:44224842023Protective Role of against Flies' Neurodegeneration with Improved Mitochondrial Function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuoliang Xiang,Xueyi Wen,Wenjing Wang,Tianchan Peng,Jiazhen Wang,Qinghua Li,Junfang Teng,Ying CuiPubMed 37868355
- PLoS genetics 19:e10107932023Yeast NDI1 reconfigures neuronal metabolism and prevents the unfolded protein response in mitochondrial complex I deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucy Granat,Debbra Y Knorr,Daniel C Ranson,Emma L Hamer,Ram Prosad Chakrabarty,Francesca Mattedi,Laura Fort-Aznar,Frank Hirth,Sean T Sweeney,Alessio Vagnoni,Navdeep S Chandel,Joseph M BatemanPubMed 37399212
- eLife 12:2023Resting mitochondrial complex I from adopts a helix-locked state.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbhilash Padavannil,Anjaneyulu Murari,Shauna-Kay Rhooms,Edward Owusu-Ansah,James A LettsPubMed 36952377
- Cellular and molecular life sciences : CMLS 80:1002023Loss of Kmt2c in vivo leads to EMT, mitochondrial dysfunction and improved response to lapatinib in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNikiana Simigdala,Anna Chalari,Aimilia D Sklirou,Evangelia Chavdoula,George Papafotiou,Pelagia Melissa,Aimilia Kafalidou,Nikolaos Paschalidis,Ioannis S Pateras,Emmanouil Athanasiadis,Dimitris Konstantopoulos,Ioannis P Trougakos,Apostolos KlinakisPubMed 36933062
- Scientific reports 12:224332022The membrane domain of respiratory complex I accumulates during muscle aging in Drosophila melanogaster.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaniz Fatima Binte Hossain,Anjaneyulu Murari,Bibhuti Mishra,Edward Owusu-AnsahPubMed 36575244
- Nature metabolism 4:1336-13512022COX7A2L genetic variants determine cardiorespiratory fitness in mice and human.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiorgia Benegiamo,Maroun Bou Sleiman,Martin Wohlwend,Sandra Rodríguez-López,Ludger J E Goeminne,Pirkka-Pekka Laurila,Marie Klevjer,Minna K Salonen,Jari Lahti,Pooja Jha,Sara Cogliati,José Antonio Enriquez,Ben M Brumpton,Anja Bye,Johan G Eriksson,Johan AuwerxPubMed 36253618
- EMBO reports 23:e548252022CG7630 is the Drosophila melanogaster homolog of the cytochrome c oxidase subunit COX7B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichele Brischigliaro,Alfredo Cabrera-Orefice,Mattia Sturlese,Dei M Elurbe,Elena Frigo,Erika Fernandez-Vizarra,Stefano Moro,Martijn A Huynen,Susanne Arnold,Carlo Viscomi,Massimo ZevianiPubMed 35699132
- The Journal of cell biology 221:2022The UPRmt preserves mitochondrial import to extend lifespan.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Xin,Jenni Durieux,Chunxia Yang,Suzanne Wolff,Hyun-Eui Kim,Andrew DillinPubMed 35608535
- Science advances 8:eabl87162022IDH2-mediated regulation of the biogenesis of the oxidative phosphorylation system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnjaneyulu Murari,Naga S V Goparaju,Shauna-Kay Rhooms,Kaniz F B Hossain,Felix G Liang,Christian J Garcia,Cindy Osei,Tong Liu,Hong Li,Richard N Kitsis,Rajesh Patel,Edward Owusu-AnsahPubMed 35544578
- Human molecular genetics 31:2623-26382022Ubiquitination at the lysine 27 residue of the Parkin ubiquitin-like domain is suggestive of a new mechanism of Parkin activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Yi Liu,Tsuyoshi Inoshita,Kahori Shiba-Fukushima,Shigeharu Yoshida,Kosuke Ogata,Yasushi Ishihama,Yuzuru Imai,Nobutaka HattoriPubMed 35313349
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| Flow Cyt | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
| Cow | Expected | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg for 106 Cells | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species Cow | Dilution info 1 µg for 106 Cells | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info 1 µg for 106 Cells | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:14729820, PubMed:30140060). Essential for the catalytic activity and assembly of complex I (PubMed:14729820, PubMed:24028823, PubMed:30140060).
Alternative names
Complex I-30kD, NADH-ubiquinone oxidoreductase 30 kDa subunit, CI-30kD, NDUFS3
Recommended products
Mouse Monoclonal NDUFS3 antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse, Cow, Rat samples. Cited in 108 publications.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 17D95
- Purification technique
- Affinity purification Immunogen
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NADH:ubiquinone oxidoreductase core subunit S3 (NDUFS3) is an important component of the mitochondrial respiratory chain complex I also known as NADH:ubiquinone oxidoreductase. With an approximate mass of 25 kDa NDUFS3 plays an integral role in the assembly and function of complex I. This protein is mostly expressed in the mitochondria across various tissues. It is better known as a core subunit important for the catalytic activity of complex I.
- Biological function summary
NDUFS3 participates in cellular respiration by facilitating the electron transfer from NADH to ubiquinone. It is part of the complex I assembly that comprises 45 different subunits. This large assembly is the first enzyme of the mitochondrial electron transport chain and it ensures efficient energy production in the form of ATP. In its role NDUFS3 collaborates closely with other core subunits like NDUFS1 and NDUFS2 to maintain the proper function of cellular metabolism.
- Pathways
NDUFS3 is essential in the oxidative phosphorylation pathway which plays an important part in ATP generation. By interacting with other complex I subunits NDUFS3 enables electron flow that drives ATP synthase activity. Additionally it is connected to the apoptosis pathway. Its dysfunction may result in disrupted energy metabolism which can trigger cell death. NDUFS3 also interacts with proteins like NDUFV1 and NDUFV2 highlighting its centrality in energy and signal transduction processes.
- Associated diseases and disorders
NDUFS3 links to mitochondrial disorders and neurodegenerative diseases such as Leigh syndrome. Mutations in NDUFS3 can impair mitochondrial function leading to reduced ATP production and accumulation of defective mitochondria. These alterations contribute to the progressive deterioration seen in these disorders. In the context of neurodegenerative diseases NDUFS3 dysfunction may associate with proteins like cytochrome c emphasizing its role in mitochondria-dependent apoptotic pathways and neuronal health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-NDUFS3 antibody [17D95] (ab14711), expandable thumbnail](https://content.abcam.com/products/images/ndufs3-antibody-17d95-ab14711--flow-cytometry-img50760.jpg "Flow Cytometry - Anti-NDUFS3 antibody [17D95] (ab14711)")
Flow Cytometry - Anti-NDUFS3 antibody [17D95] (ab14711)
Overlay histogram showing HepG2 cells stained with ab14711 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14711, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![Western blot - Anti-NDUFS3 antibody [17D95] (ab14711), expandable thumbnail](https://content.abcam.com/products/images/ndufs3-antibody-17d95-ab14711--western-blot-img80146.jpg "Western blot - Anti-NDUFS3 antibody [17D95] (ab14711)")
Western blot - Anti-NDUFS3 antibody [17D95] (ab14711)
The band observed at 26 kDa could potentially be a cleaved form of NDUFS3 due to the presence of a 36 amino acid transit peptide.
All lanes: Western blot - Anti-NDUFS3 antibody [17D95] (ab14711) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: Human heart tissue lysate - total protein (ab29431) at 10 µg
Lane 3: Heart (Mouse) Tissue Lysate at 10 µg
Lane 4: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 26 kDa, 57 kDa
Exposure time: 1min
![Western blot - Anti-NDUFS3 antibody [17D95] (ab14711), expandable thumbnail](https://content.abcam.com/products/images/ndufs3-antibody-17d95-ab14711--western-blot-img7436.jpg "Western blot - Anti-NDUFS3 antibody [17D95] (ab14711)")
Image is courtesy of an anonymous customer reviewWestern blot - Anti-NDUFS3 antibody [17D95] (ab14711)
All lanes: Western blot - Anti-NDUFS3 antibody [17D95] (ab14711) at 1/1000 dilution
Lanes 1 - 2: Whole cell lysates from NDUFS3 KO cells. at 50 µg
Lanes 3 - 4: Whole cell lysates from NDUFS3 WT cells. at 50 µg
Lanes 5 - 6: Isolated mitochondria from NDUFS3 KO cells. at 50 µg
Lanes 7 - 8: Isolated mitochondria from NDUFS3 WT cells. at 50 µg
SecondaryAll lanes: Goat anti-mouse polyclonal HRP conjugate at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 22 kDa, 40 kDa
Exposure time: 1min
![Western blot - Anti-NDUFS3 antibody [17D95] (ab14711), expandable thumbnail](https://content.abcam.com/products/images/ndufs3-antibody-17d95-ab14711--western-blot-img102336.jpg "Western blot - Anti-NDUFS3 antibody [17D95] (ab14711)")
Western blot - Anti-NDUFS3 antibody [17D95] (ab14711)
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
All lanes: Western blot - Anti-NDUFS3 antibody [17D95] (ab14711)
Lane 1: Isolated mitochondria from Human heart at 5 µg
Lane 2: Isolated mitochondria from Bovine heart at 4 µg
Lane 3: Isolated mitochondria from Rat heart at 10 µg
Lane 4: Isolated mitochondria from Mouse heart at 10 µg
SecondaryAll lanes: Goat anti-Mouse IgG
Predicted band size: 30 kDa
Observed band size: 26 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com