Anti-NDUFS3 antibody [3F9DD2]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (AB110246)

ab110246, at 1/1000, staining NDUFS3 in formalin fixed and paraffin embedded Human cerebellum tissue by Immunohistochemistry. ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated with the target for 1 hour. Antigen retrieval performed by 1 min pressure cooking with 1mmol EDTA, pH8.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NDUFS3 antibody [3F9DD2] (AB110246)

ab110246, at 10 µg/ml, staining NDUFS3 in cultured Human fibroblasts by Immunofluorescence. The cells were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110246, followed by a fluorescent goat-anti-mouse IgG.

• Flow Cyt

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Flow Cytometry - Anti-NDUFS3 antibody [3F9DD2] (AB110246)

ab110246, at 1 µg/ml, staining NDUFS3 in HeLa cells by Flow Cytometry (Blue). Isotype control antibody, at 1 µg/ml, staining (red).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (AB110246)

ab110246, at 1/100, staining NDUFS3 in Human colon tissue fixed with 4% PFA by Immunohistochemistry. ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated for 1 hour with the target. Antigen retrieval was not required.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (AB110246)

ab110246 staining NDUFS3 in Human colon tissue sections from a normal ageing patient by Immunohistochemistry. Kindly provided by Dr. L. Greaves and D. Turnbull, Mitochondrial Research Group, Newcastle University. For more details, see Taylor et al., J. Clin. Invest. 112 : 1351-1360 (2003).

• WB

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Western blot - Anti-NDUFS3 antibody [3F9DD2] (AB110246)

All lanes:

Western blot - Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 0.5 µg/mL

Lane 1:

Isolated mitochondria from Human heart at 5 µg

Lane 2:

Isolated mitochondria from Bovine heart at 1 µg

Lane 3:

Isolated mitochondria from Rat heart at 10 µg

Lane 4:

Isolated mitochondria from Mouse heart at 10 µg

Predicted band size: 30 kDa

false

• WB

AbReview51456****

Western blot - Anti-NDUFS3 antibody [3F9DD2] (AB110246)

All lanes:

Western blot - Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 1/1000 dilution

Lanes 1 - 2:

Whole cell lysates from NDUFS3 KO cells. at 50 µg

Lanes 3 - 4:

Whole cell lysates from NDUFS3 WT cells. at 50 µg

Lanes 5 - 6:

Isolated mitochondria from NDUFS3 KO cells. at 50 µg

Lanes 7 - 8:

Isolated mitochondria from NDUFS3 WT cells. at 50 µg

Secondary

All lanes:

Goat anti-Mouse monoclonal HRP conjugate at 1/5000 dilution

Predicted band size: 30 kDa

Observed band size: 260 kDa,40 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous AbReview