AB87399

Anti-Ndufs4 antibody [2C7CD4AG3]

Name: Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399) | Abcam
Brand: Abcam Limited
SKU: AB87399
Price: 485 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(23 Publications)

Mouse Monoclonal Ndufs4 antibody. Suitable for Flow Cyt, WB and reacts with Human, Cow, Rat, Mouse samples. Cited in 23 publications.

View Alternative Names

Complex I-18 kDa, Complex I-AQDQ, NADH-ubiquinone oxidoreductase 18 kDa subunit, CI-18 kDa, CI-AQDQ, NDUFS4

2 Images

Flow Cytometry - Anti-Ndufs4 antibody [2C7CD4AG3] (AB87399)

Flow Cyt

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Flow Cytometry - Anti-Ndufs4 antibody [2C7CD4AG3] (AB87399)

Overlay histogram showing HepG2 cells stained with ab87399 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87399, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

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Western blot - Anti-Ndufs4 antibody [2C7CD4AG3] (AB87399)

All lanes:

Western blot - Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)

Lane 1:

Isolated mitochondria from human heart at 15 µg

Lane 2:

Isolated mitochondria from bovine heart at 6 µg

Lane 3:

Isolated mitochondria from rat heart at 30 µg

Lane 4:

Isolated mitochondria from mouse heart at 30 µg

Predicted band size: 20 kDa

Observed band size: 20 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2C7CD4AG3

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Cow, Human

Applications

WB, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES, Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ndufs4 also known as NADH:Ubiquinone Oxidoreductase Subunit S4 is an important component of mitochondrial complex I which is part of the electron transport chain. It has a molecular mass of approximately 18 kDa. The protein is mainly found in the inner mitochondrial membrane where it plays an essential role in cellular respiration by transferring electrons from NADH to ubiquinone. Ndufs4 is widely expressed in tissues with high energy demands such as the brain and heart.

Biological function summary

Ndufs4 functions as a part of mitochondrial complex I a large protein enzyme complex critical for oxidative phosphorylation. The complex comprises multiple subunits of which Ndufs4 is a small but essential part. By participating in electron transfer and proton pumping Ndufs4 supports ATP synthesis which is the main energy currency of the cell. Deficiency in Ndufs4 leads to dysfunctional energy production linked with metabolic disorders.

Pathways

The protein is an integral part of the respiratory chain pathway where it supports oxidative phosphorylation enabling effective ATP production. Within these pathways Ndufs4 interacts with other complex I subunits including Ndufs1 and Ndufa9 which stabilize electron transport. Disruptions in this chain can affect cellular energy metabolism and have downstream effects on various cellular processes.

Mutations in the Ndufs4 gene are associated with Leigh syndrome and mitochondrial complex I deficiency. Leigh syndrome is a severe neurological disorder characterized by progressive loss of mental and movement abilities. Ndufs4 mutations also lead to complex I deficiency causing diverse symptoms ranging from neurological impairments to muscle weakness. These disorders highlight the critical link between Ndufs4 and proper mitochondrial function. The protein's connection to other complex I subunits such as Ndufs1 emphasizes its importance in maintaining regular cellular functions and preventing mitochondrial diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

See full target information NDUFS4

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 28:1844-1857 PubMed40790270

2025

Potentiation of mitochondrial function by mitoDREADD-G reverses pharmacological and neurodegenerative cognitive impairment in mice.

Applications

Species

Unspecified reactive species

Antonio C Pagano Zottola,Rebeca Martín-Jiménez,Gianluca Lavanco,Geneviève Hamel-Côté,Carla Ramon-Duaso,Rui S Rodrigues,Yamuna Mariani,Mehtab Khan,Filippo Drago,Stephanie Jean,Itziar Bonilla-Del Río,Daniel Jimenez-Blasco,Jon Egaña-Huguet,Abel Eraso-Pichot,Sandra Beriain,Astrid Cannich,Laura Vidal-Palencia,Rosmara Infantino,Francisca Julio-Kalajzić,Doriane Gisquet,Ania Goncalves,Inas Al-Younis,Yann Baussan,Stephane Duvezin-Caubet,Anne Devin,Edgar Soria-Gomez,Nagore Puente,Juan P Bolaños,Pedro Grandes,Sandrine Pouvreau,Arnau Busquets-Garcia,Giovanni Marsicano,Luigi Bellocchio,Etienne Hebert-Chatelain

Nature communications 15:7730 PubMed39231983

2024

Cannabidiol ameliorates mitochondrial disease via PPARγ activation in preclinical models.

Applications

Unspecified application

Species

Unspecified reactive species

Emma Puighermanal,Marta Luna-Sánchez,Alejandro Gella,Gunter van der Walt,Andrea Urpi,María Royo,Paula Tena-Morraja,Isabella Appiah,Maria Helena de Donato,Fabien Menardy,Patrizia Bianchi,Anna Esteve-Codina,Laura Rodríguez-Pascau,Cristina Vergara,Mercè Gómez-Pallarès,Giovanni Marsicano,Luigi Bellocchio,Marc Martinell,Elisenda Sanz,Sandra Jurado,Francesc Xavier Soriano,Pilar Pizcueta,Albert Quintana

Cells 11: PubMed36010658

2022

Expression of Functional Cannabinoid Type-1 (CB) Receptor in Mitochondria of White Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio C Pagano Zottola,Ilenia Severi,Astrid Cannich,Philippe Ciofi,Daniela Cota,Giovanni Marsicano,Antonio Giordano,Luigi Bellocchio

Gene therapy 29:368-378 PubMed35383288

2022

Gene therapy restores mitochondrial function and protects retinal ganglion cells in optic neuropathy induced by a mito-targeted mutant ND1 gene.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Liu,Jeremy D Eastwood,Diego E Alba,Sindhu Velmurugan,Ning Sun,Vittorio Porciatti,Richard K Lee,William W Hauswirth,John Guy,Hong Yu

PLoS biology 19:e3001252 PubMed33983919

2021

Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition.

Applications

Unspecified application

Species

Unspecified reactive species

Pau B Esparza-Moltó,Inés Romero-Carramiñana,Cristina Núñez de Arenas,Marta P Pereira,Noelia Blanco,Beatriz Pardo,Georgina R Bates,Carla Sánchez-Castillo,Rafael Artuch,Michael P Murphy,José A Esteban,José M Cuezva

Cell reports methods 1:100002 PubMed35474694

2021

Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Fabio Bertan,Lena Wischhof,Enzo Scifo,Mihaela Guranda,Joshua Jackson,Anaïs Marsal-Cots,Antonia Piazzesi,Miriam Stork,Michael Peitz,Jochen Herbert Martin Prehn,Dan Ehninger,Pierluigi Nicotera,Daniele Bano

Nature 586:287-291 PubMed32728214

2020

Na controls hypoxic signalling by the mitochondrial respiratory chain.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Hernansanz-Agustín,Carmen Choya-Foces,Susana Carregal-Romero,Elena Ramos,Tamara Oliva,Tamara Villa-Piña,Laura Moreno,Alicia Izquierdo-Álvarez,J Daniel Cabrera-García,Ana Cortés,Ana Victoria Lechuga-Vieco,Pooja Jadiya,Elisa Navarro,Esther Parada,Alejandra Palomino-Antolín,Daniel Tello,Rebeca Acín-Pérez,Juan Carlos Rodríguez-Aguilera,Plácido Navas,Ángel Cogolludo,Iván López-Montero,Álvaro Martínez-Del-Pozo,Javier Egea,Manuela G López,John W Elrod,Jesús Ruíz-Cabello,Anna Bogdanova,José Antonio Enríquez,Antonio Martínez-Ruiz

Nature 583:603-608 PubMed32641832

2020

Glucose metabolism links astroglial mitochondria to cannabinoid effects.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Jimenez-Blasco,Arnau Busquets-Garcia,Etienne Hebert-Chatelain,Roman Serrat,Carlos Vicente-Gutierrez,Christina Ioannidou,Paula Gómez-Sotres,Irene Lopez-Fabuel,Monica Resch-Beusher,Eva Resel,Dorian Arnouil,Dave Saraswat,Marjorie Varilh,Astrid Cannich,Francisca Julio-Kalajzic,Itziar Bonilla-Del Río,Angeles Almeida,Nagore Puente,Svein Achicallende,Maria-Luz Lopez-Rodriguez,Charlotte Jollé,Nicole Déglon,Luc Pellerin,Charlène Josephine,Gilles Bonvento,Aude Panatier,Beat Lutz,Pier-Vincenzo Piazza,Manuel Guzmán,Luigi Bellocchio,Anne-Karine Bouzier-Sore,Pedro Grandes,Juan P Bolaños,Giovanni Marsicano

Brain : a journal of neurology 143:1686-1696 PubMed32413099

2020

Gene replacement therapy provides benefit in an adult mouse model of Leigh syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Robin Reynaud-Dulaurier,Giorgia Benegiamo,Elena Marrocco,Racha Al-Tannir,Enrico Maria Surace,Johan Auwerx,Michael Decressac

Nature communications 11:1643 PubMed32242014

2020

A salvage pathway maintains highly functional respiratory complex I.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Szczepanowska,Katharina Senft,Juliana Heidler,Marija Herholz,Alexandra Kukat,Michaela Nicole Höhne,Eduard Hofsetz,Christina Becker,Sophie Kaspar,Heiko Giese,Klaus Zwicker,Sergio Guerrero-Castillo,Linda Baumann,Johanna Kauppila,Anastasia Rumyantseva,Stefan Müller,Christian K Frese,Ulrich Brandt,Jan Riemer,Ilka Wittig,Aleksandra Trifunovic

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ndufs4 antibody [2C7CD4AG3]

Flow Cytometry - Anti-Ndufs4 antibody [2C7CD4AG3] (AB87399)

Western blot - Anti-Ndufs4 antibody [2C7CD4AG3] (AB87399)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

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