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Rabbit Recombinant Monoclonal Ndufs4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (AB248861), expandable thumbnail
  • Western blot - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (AB248861), expandable thumbnail
  • Western blot - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (AB248861), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (AB248861), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (AB248861), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Expected
Rat
Expected
Not recommended
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Use of a HRP/AP polymerized secondary antibody is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
-
Notes

Use of a HRP/AP polymerized secondary antibody is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Use of a HRP/AP polymerized secondary antibody is recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternative names

Complex I-18 kDa, Complex I-AQDQ, NADH-ubiquinone oxidoreductase 18 kDa subunit, CI-18 kDa, CI-AQDQ, NDUFS4

Recommended products

Rabbit Recombinant Monoclonal Ndufs4 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7831
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248861 is the carrier-free version of Anti-Ndufs4 antibody [EPR7831] ab139178.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ndufs4 also known as NADH:Ubiquinone Oxidoreductase Subunit S4 is an important component of mitochondrial complex I which is part of the electron transport chain. It has a molecular mass of approximately 18 kDa. The protein is mainly found in the inner mitochondrial membrane where it plays an essential role in cellular respiration by transferring electrons from NADH to ubiquinone. Ndufs4 is widely expressed in tissues with high energy demands such as the brain and heart.

Biological function summary

Ndufs4 functions as a part of mitochondrial complex I a large protein enzyme complex critical for oxidative phosphorylation. The complex comprises multiple subunits of which Ndufs4 is a small but essential part. By participating in electron transfer and proton pumping Ndufs4 supports ATP synthesis which is the main energy currency of the cell. Deficiency in Ndufs4 leads to dysfunctional energy production linked with metabolic disorders.

Pathways

The protein is an integral part of the respiratory chain pathway where it supports oxidative phosphorylation enabling effective ATP production. Within these pathways Ndufs4 interacts with other complex I subunits including Ndufs1 and Ndufa9 which stabilize electron transport. Disruptions in this chain can affect cellular energy metabolism and have downstream effects on various cellular processes.

Associated diseases and disorders

Mutations in the Ndufs4 gene are associated with Leigh syndrome and mitochondrial complex I deficiency. Leigh syndrome is a severe neurological disorder characterized by progressive loss of mental and movement abilities. Ndufs4 mutations also lead to complex I deficiency causing diverse symptoms ranging from neurological impairments to muscle weakness. These disorders highlight the critical link between Ndufs4 and proper mitochondrial function. The protein's connection to other complex I subunits such as Ndufs1 emphasizes its importance in maintaining regular cellular functions and preventing mitochondrial diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861), expandable thumbnail

    Western blot - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861)

    This data was developed using Anti-Ndufs4 antibody [EPR7831] ab139178, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 μg)

    Lane 2: Ndufs4 knockout HAP1 cell lysate (20 μg)

    Lane 3: HEK293 cell lysate (20 μg)

    Lane 4: Human fetal heart tissue lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-Ndufs4 antibody [EPR7831] ab139178 observed at 23 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    Anti-Ndufs4 antibody [EPR7831] ab139178 was shown to recognize Ndufs4 when Ndufs4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ndufs4 knockout samples were subjected to SDS-PAGE. Anti-Ndufs4 antibody [EPR7831] ab139178 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Ndufs4 antibody [EPR7831] (Anti-Ndufs4 antibody [EPR7831] ab139178)

    Predicted band size: 20 kDa

  • Western blot - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861), expandable thumbnail

    Western blot - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861)

    This data was developed using Anti-Ndufs4 antibody [EPR7831] ab139178, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Ndufs4 antibody [EPR7831] (Anti-Ndufs4 antibody [EPR7831] ab139178) at 1/1000 dilution

    Lane 1: Human fetal heart tissue lysate at 10 µg

    Lane 2: Human fetal kidney tissue lysate at 10 µg

    Lane 3: Human fetal brain tissue lysate at 10 µg

    Lane 4: 293T cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit

    Predicted band size: 20 kDa

  • Western blot - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861), expandable thumbnail

    Western blot - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861)

    This data was developed using Anti-Ndufs4 antibody [EPR7831] ab139178, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Ndufs4 antibody [EPR7831] (Anti-Ndufs4 antibody [EPR7831] ab139178) at 1/1000 dilution

    Lane 1: Mouse heart tissue lysate at 10 µg

    Lane 2: Mouse kidney tissue lysate at 10 µg

    Lane 3: Rat kidney tissue lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 20 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861)

    This data was developed using Anti-Ndufs4 antibody [EPR7831] ab139178, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Ndufs4 with Anti-Ndufs4 antibody [EPR7831] ab139178 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs4 antibody [EPR7831] - BSA and Azide free (ab248861)

    This data was developed using Anti-Ndufs4 antibody [EPR7831] ab139178, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human stomach tissue labelling Ndufs4 with Anti-Ndufs4 antibody [EPR7831] ab139178 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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