AB179806

Anti-NDUFS5 antibody [EPR12291]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal NDUFS5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, Complex I-15 kDa, NADH-ubiquinone oxidoreductase 15 kDa subunit, CI-15 kDa, NDUFS5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS5 antibody [EPR12291] (AB179806)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS5 antibody [EPR12291] (AB179806)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling NDUFS5 with ab179806 at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-NDUFS5 antibody [EPR12291] (AB179806)

All lanes:

Western blot - Anti-NDUFS5 antibody [EPR12291] (ab179806) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

HUVEC lysate at 10 µg

Lane 4:

K562 lysate at 10 µg

Lane 5:

HepG2 lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 13 kDa

false

Carrier free

Anti-NDUFS5 antibody [EPR12291] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12291

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFS5 also known as NADH:Ubiquinone Oxidoreductase Subunit S5 forms a part of the mitochondrial complex I. This protein has a molecular mass of approximately 18 kDa. NDUFS5 is expressed in cells across various tissues including high levels in muscle tissues and neurons. The protein assists in the initial stages of the electron transport chain an essential process for cellular respiration and ATP production.

Biological function summary

NDUFS5 contributes to the electron transfer chain by participating in complex I. Complex I plays a central role in converting NADH to NAD+ facilitating the transport of electrons to ubiquinone. NDUFS5 is not a catalytic subunit but supports the overall structure and function of the complex working together with other subunits to maintain the electron flow essential for ATP synthesis.

Pathways

NDUFS5 actively participates in the oxidative phosphorylation pathway. This pathway drives the production of ATP the primary energy source for cells. NDUFS5 collaborates with other proteins like NDUFS2 and NDUFV1 in complex I of the electron transport chain. Additionally NDUFS5 contributes to pathways related to reactive oxygen species (ROS) production influencing cellular stress responses.

NDUFS5 is linked to mitochondrial dysfunctions including Leigh syndrome and mitochondrial encephalomyopathy. These conditions arise from defects in complex I in which NDUFS5 and proteins like MT-ND1 and NDUFS4 are involved. Such disruptions lead to impaired ATP production and increased oxidative stress affecting energy-intensive tissues like the brain and muscles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

See full target information NDUFS5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 26:759-774.e5 PubMed30650365

2019

Vectorial Import via a Metastable Disulfide-Linked Complex Allows for a Quality Control Step and Import by the Mitochondrial Disulfide Relay.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Habich,Silja Lucia Salscheider,Lena Maria Murschall,Michaela Nicole Hoehne,Manuel Fischer,Fabian Schorn,Carmelina Petrungaro,Muna Ali,Alican J Erdogan,Shadi Abou-Eid,Hamid Kashkar,Joern Dengjel,Jan Riemer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUFS5 antibody [EPR12291]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS5 antibody [EPR12291] (AB179806)

Western blot - Anti-NDUFS5 antibody [EPR12291] (AB179806)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise