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Rabbit Recombinant Monoclonal NDUFS6 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (AB251211), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (AB251211), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (AB251211), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (AB251211), expandable thumbnail
  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (AB251211), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Expected
Tested
Mouse
Tested
Expected
Tested
Expected
Rat
Tested
Expected
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternative names

Complex I-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit, CI-13kD-A, NDUFS6

Recommended products

Rabbit Recombinant Monoclonal NDUFS6 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR15957-37
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251211 is the carrier-free version of Anti-NDUFS6 antibody [EPR15957-37] ab195808.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NDUFS6 also known as NADH:ubiquinone oxidoreductase subunit S6 serves a critical role in mitochondrial function. It forms a component of the mitochondrial complex I which is the first enzyme of the electron transport chain. NDUFS6 has a molecular weight of approximately 15 kDa. Researchers found this protein expressed in a variety of tissues though it is typically higher in metabolically active tissues such as heart liver and skeletal muscles.

Biological function summary

NDUFS6 contributes to the generation of ATP by participating in oxidative phosphorylation. It is part of the mitochondrial complex I a multisubunit assembly essential for electron transfer from NADH to ubiquinone. The proper function of this subunit is necessary for the activity of complex I which significantly impacts the production of cellular energy. Disruptions in this protein's function can impair energy metabolism affecting numerous cell processes.

Pathways

NDUFS6 plays a role in the electron transport chain and oxidative phosphorylation pathway. By facilitating electron transfer it helps maintain the electrochemical gradient across the inner mitochondrial membrane which is important for ATP synthesis. NDUFS6 works closely with other complex I proteins like NDUFS1 and NDUFS7 ensuring efficient energy production and cellular respiration. Its activity impacts broader cellular processes reflecting in energy homeostasis and cellular growth.

Associated diseases and disorders

NDUFS6 mutations have connections with mitochondrial diseases and disorders like Leigh syndrome. This protein's dysfunction can lead to defects in energy generation resulting in neuromuscular symptoms and systemic issues. Researchers also correlate improper NDUFS6 function with complex I deficiencies where other proteins such as NDUFS1 are often implicated. Understanding the role of NDUFS6 in these disorders aids the investigation of mitochondrial dysfunction and offers pathways for potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Flow Cytometry (Intracellular) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

    This data was developed using Anti-NDUFS6 antibody [EPR15957-37] ab195808, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of NIH 3T3 cells (paraformaldehyde-fixed, 2%) labeling NDUFS6 with Anti-NDUFS6 antibody [EPR15957-37] ab195808 at 1/90 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

    This data was developed using Anti-NDUFS6 antibody [EPR15957-37] ab195808, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (100% methanol-fixed, 0.1% tritonX-100 permeabilized) labeling NDUFS6 with Anti-NDUFS6 antibody [EPR15957-37] ab195808 at 1/200 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).Note: Cytoplasm staining on HeLa cell line was observed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

    This data was developed using Anti-NDUFS6 antibody [EPR15957-37] ab195808, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix squamous cell carcinoma tissue labeling NDUFS6 with Anti-NDUFS6 antibody [EPR15957-37] ab195808 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control). Note: Cytoplasm staining on human squamous cell carcinoma of cervix tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

    This data was developed using Anti-NDUFS6 antibody [EPR15957-37] ab195808, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFS6 with Anti-NDUFS6 antibody [EPR15957-37] ab195808 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control). Note: Cytoplasm staining on human kidney tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211), expandable thumbnail

    Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

    This data was developed using Anti-NDUFS6 antibody [EPR15957-37] ab195808, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-NDUFS6 antibody [EPR15957-37] (Anti-NDUFS6 antibody [EPR15957-37] ab195808) at 1/1000 dilution

    All lanes: PC-12 cell lysate

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 13 kDa

    Exposure time: 3min

  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211), expandable thumbnail

    Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

    This data was developed using Anti-NDUFS6 antibody [EPR15957-37] ab195808, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-NDUFS6 antibody [EPR15957-37] (Anti-NDUFS6 antibody [EPR15957-37] ab195808) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Mouse heart tissue lysate at 10 µg

    Lane 3: Mouse kidney tissue lysate at 10 µg

    Lane 4: Rat brain tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 13 kDa

    Exposure time: 10s

  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211), expandable thumbnail

    Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

    This data was developed using Anti-NDUFS6 antibody [EPR15957-37] ab195808, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-NDUFS6 antibody [EPR15957-37] (Anti-NDUFS6 antibody [EPR15957-37] ab195808) at 1/10000 dilution

    Lane 1: Human fetal heart lysate at 20 µg

    Lane 2: Human skeletal muscle lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 13 kDa

    Exposure time: 30s

  • Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211), expandable thumbnail

    Western blot - Anti-NDUFS6 antibody [EPR15957-37] - BSA and Azide free (ab251211)

    This data was developed using Anti-NDUFS6 antibody [EPR15957-37] ab195808, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-NDUFS6 antibody [EPR15957-37] (Anti-NDUFS6 antibody [EPR15957-37] ab195808) at 1/10000 dilution

    Lane 1: Human fetal kidney lysate at 20 µg

    Lane 2: Human fetal brain lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 13 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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