AB251210

Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal NDUFS6 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Complex I-13kD-A, NADH-ubiquinone oxidoreductase 13 kDa-A subunit, CI-13kD-A, NDUFS6

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

This data was developed using ab195807, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NDUFS6 with ab195807 at 1/500. Secondary antibody : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500. Inset image : negative control obtained using PBS instead of ab195807 and secondary antibody only.
Note : Cytoplasm staining on human tonsil tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

This data was developed using ab195807, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling NDUFS6 with ab195807 at 1/500. Secondary antibody : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

Inset image : negative control obtained using PBS instead of ab195807 and secondary antibody only.
Note : Cytoplasm staining on human hepatocellular carcinoma tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

This data was developed using ab195807, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of Human fetal kidney lysate labeling NDUFS6 using ab195807 at 1/30 dilution (Lane 2).

Lane 3 : IP using Rabbit monoclonal IgG (ab172730) ) instead of ab195807 in Human fetal kidney lysates.

Lane 1 : Input : 10 μg Human fetal kidney lysates.

Subsequent WB detection was performed using ab195807 at 1/1000 dilution.

An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-NDUFS6 antibody [EPR15957] (<a href='/en-us/products/primary-antibodies/ndufs6-antibody-epr15957-ab195807'>ab195807</a>)

Predicted band size: 13 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

This data was developed using ab195807, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling NDUFS6 with ab195807 at 1/500. Secondary antibody : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

Inset image : negative control obtained using PBS instead of ab195807 and secondary antibody only.
Note : Cytoplasm staining on rat liver tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

This data was developed using ab195807, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUFS6 antibody [EPR15957] (<a href='/en-us/products/primary-antibodies/ndufs6-antibody-epr15957-ab195807'>ab195807</a>) at 1/10000 dilution

All lanes:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 13 kDa

false

Supplier Data

Western blot - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

This data was developed using ab195807, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUFS6 antibody [EPR15957] (<a href='/en-us/products/primary-antibodies/ndufs6-antibody-epr15957-ab195807'>ab195807</a>) at 1/10000 dilution

All lanes:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 13 kDa

false

Supplier Data

Western blot - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

This data was developed using ab195807, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUFS6 antibody [EPR15957] (<a href='/en-us/products/primary-antibodies/ndufs6-antibody-epr15957-ab195807'>ab195807</a>) at 1/10000 dilution

All lanes:

Human fetal heart at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 13 kDa

false

Unknown

Western blot - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

This data was developed using ab195807, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NDUFS6 antibody [EPR15957] (<a href='/en-us/products/primary-antibodies/ndufs6-antibody-epr15957-ab195807'>ab195807</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Rat brain lysate at 10 µg

Lane 4:

Rat heart lysate at 10 µg

Lane 5:

Rat kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 13 kDa

false

Unconjugated

Anti-NDUFS6 antibody [EPR15957]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15957

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab251210 is the carrier-free version of ab195807.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFS6 also known as NADH:ubiquinone oxidoreductase subunit S6 serves a critical role in mitochondrial function. It forms a component of the mitochondrial complex I which is the first enzyme of the electron transport chain. NDUFS6 has a molecular weight of approximately 15 kDa. Researchers found this protein expressed in a variety of tissues though it is typically higher in metabolically active tissues such as heart liver and skeletal muscles.

Biological function summary

NDUFS6 contributes to the generation of ATP by participating in oxidative phosphorylation. It is part of the mitochondrial complex I a multisubunit assembly essential for electron transfer from NADH to ubiquinone. The proper function of this subunit is necessary for the activity of complex I which significantly impacts the production of cellular energy. Disruptions in this protein's function can impair energy metabolism affecting numerous cell processes.

Pathways

NDUFS6 plays a role in the electron transport chain and oxidative phosphorylation pathway. By facilitating electron transfer it helps maintain the electrochemical gradient across the inner mitochondrial membrane which is important for ATP synthesis. NDUFS6 works closely with other complex I proteins like NDUFS1 and NDUFS7 ensuring efficient energy production and cellular respiration. Its activity impacts broader cellular processes reflecting in energy homeostasis and cellular growth.

NDUFS6 mutations have connections with mitochondrial diseases and disorders like Leigh syndrome. This protein's dysfunction can lead to defects in energy generation resulting in neuromuscular symptoms and systemic issues. Researchers also correlate improper NDUFS6 function with complex I deficiencies where other proteins such as NDUFS1 are often implicated. Understanding the role of NDUFS6 in these disorders aids the investigation of mitochondrial dysfunction and offers pathways for potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

See full target information NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 20:1079-1090 PubMed37484809

2023

NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfei Long,Yifei Ren,Qinglv Wei,Youchaou Mobet,Yujiao Liu,Hongyan Zhao,Tao Liu,Lei Cheng,Ping Yi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

Immunoprecipitation - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

Western blot - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

Western blot - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

Western blot - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

Western blot - Anti-NDUFS6 antibody [EPR15957] - BSA and Azide free (AB251210)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise