Rabbit Polyclonal NDUFS8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NDUFS8 aa 150 to C-terminus.
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- Brain communications 4:fcac1022022Disruption of cellular iron homeostasis by missense variants causes severe neurodevelopmental delay, dystonia and seizures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNunziata Maio,Russell P Saneto,Richard Steet,Marcio A Sotero de Menezes,Cindy Skinner,Tracey A RouaultPubMed 35602653
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NDUFS8 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00217
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:22499348). Essential for the catalytic activity and assembly of complex I (PubMed:22499348).
Alternative names
Complex I-23kD, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY subunit, CI-23kD, NDUFS8
Recommended products
Rabbit Polyclonal NDUFS8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NDUFS8 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NDUFS8 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00217
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab241346 was affinity purified using an epitope specific to NDUFS8 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NDUFS8 protein also known as NADH:ubiquinone oxidoreductase core subunit S8 serves an important function in the mitochondrial electron transport chain. It weighs approximately 23 kDa and is expressed in many tissues due to its essential role in energy production. NDUFS8 is a part of the larger mitochondrial complex I where it plays a role in transferring electrons derived from NADH to ubiquinone. Through this process NDUFS8 contributes to the creation of a proton gradient used for ATP synthesis.
- Biological function summary
The NDUFS8 protein functions as an integral component of the mitochondrial respiratory complex I. Complex I is essential for cellular respiration and contributes to the production of ATP which cells use as an energy source. NDUFS8 helps facilitate the electron transport chain as a subunit of this multi-protein complex ensuring effective electron transfer and energy conversion within the mitochondria.
- Pathways
The NDUFS8 protein operates within the oxidative phosphorylation pathway a major energy-producing pathway in cells. It also has connections with the tricarboxylic acid (TCA) cycle where NADH is generated and enters the electron transport chain through NDUFS8. The NDUFS8 protein collaborates with other subunits of complex I such as NDUFV1 and NDUFV2 to ensure proper electron flow and energy production.
- Associated diseases and disorders
Mutations in the NDUFS8 protein can lead to mitochondrial disorders such as Leigh syndrome and mitochondrial encephalomyopathy. These conditions are characterized by severe energy production deficiencies due to impaired function of complex I. NDUFS8 mutations can interact with other proteins implicated in these disorders including NDUFAF2 which is involved in the assembly of complex I further disrupting normal mitochondrial activity and energy metabolism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NDUFS8 antibody (ab241346)
Lysates prepared using NETN lysis buffer.
All lanes: Western blot - Anti-NDUFS8 antibody (ab241346) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 23 kDa
Exposure time: 30s
Immunoprecipitation - Anti-NDUFS8 antibody (ab241346)
NDUFS8 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241346 used for IP at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab241346 at 1 μg/ml.Lane 1: ab241346 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-NDUFS8 antibody (ab241346)
Predicted band size: 23 kDa
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