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Rabbit Recombinant Monoclonal NDUFV1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (AB321893), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (AB321893), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (AB321893), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (AB321893), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (AB321893), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Not recommended
Mouse
Tested
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Target data

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor.

Alternative names

Complex I-51kD, NADH dehydrogenase flavoprotein 1, NADH-ubiquinone oxidoreductase 51 kDa subunit, CI-51kD, NDUFV1, UQOR1

Recommended products

Rabbit Recombinant Monoclonal NDUFV1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR29179-78

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NDUFV1 also known as NADH-ubiquinone oxidoreductase 51 kDa subunit plays a mechanical role in the electron transport chain. It has a molecular mass of approximately 51 kDa. The protein is an integral part of Complex I or NADH:ubiquinone oxidoreductase located in the inner mitochondrial membrane. NDUFV1 shows expression across various tissues with higher levels found in metabolically active tissues like skeletal muscle and heart.

Biological function summary

NDUFV1 participates in oxidative phosphorylation within cells. This protein functions as a core subunit of Complex I which is the first enzyme in the mitochondrial respiratory chain. NDUFV1 has a role in transferring electrons from NADH to ubiquinone an essential step for energy production. The efficient operation of Complex I affects ATP synthesis which is necessary for many cellular processes.

Pathways

NDUFV1 is central to the energy production in mitochondria. It is important for the oxidative phosphorylation pathway and is involved in maintaining the proton gradient across the inner mitochondrial membrane. This protein closely interacts with other subunits of Complex I like NDUFS1 and has significant synergy with them in electron transfer processes. Its activity is indispensable for proper cellular respiration and energy metabolism.

Associated diseases and disorders

NDUFV1 mutations link to mitochondrial diseases such as Leigh syndrome and some cases of Complex I deficiency. In patients with these conditions there are disruptions in ATP production due to impaired electron transport chain function. The protein may also be involved in some forms of neurodegenerative diseases where mitochondrial dysfunction plays a role. NDUFS1 and other Complex I subunits often show associations with these disorders highlighting the importance of NDUFV1 in neuromuscular and metabolic health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (ab321893), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (ab321893)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling NDUFV1 with ab321893 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cerebrum.

    The section was incubated with ab321893 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (ab321893), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (ab321893)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NDUFV1 with ab321893 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human kidney.

    The section was incubated with ab321893 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (ab321893), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (ab321893)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling NDUFV1 with ab321893 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse cerebrum.

    The section was incubated with ab321893 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (ab321893), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (ab321893)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling NDUFV1 with ab321893 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human lung carcinoma.

    The section was incubated with ab321893 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (ab321893), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV1 antibody [EPR29179-78] (ab321893)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling NDUFV1 with ab321893 at 1/2000 (0.253 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse kidney.

    The section was incubated with ab321893 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-NDUFV1 antibody [EPR29179-78] (ab321893), expandable thumbnail

    Western blot - Anti-NDUFV1 antibody [EPR29179-78] (ab321893)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-TOMM20 (Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-NDUFV1 antibody [EPR29179-78] (ab321893) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg

    Lane 2: HeLa mitochondrial fraction at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 51 kDa, 36 kDa, 16 kDa

    Exposure time: 26s

  • Western blot - Anti-NDUFV1 antibody [EPR29179-78] (ab321893), expandable thumbnail

    Western blot - Anti-NDUFV1 antibody [EPR29179-78] (ab321893)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-NDUFV1 antibody [EPR29179-78] (ab321893) at 1/1000 dilution

    Lane 1: HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HEK-293 transfected with siRNA specifically targeting at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 51 kDa, 36 kDa

    Exposure time: 26s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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