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Rabbit Monoclonal NDUFV1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human NDUFV1.

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Images

Western blot - Anti-NDUFV1 antibody [HL1600] (AB308175), expandable thumbnail
  • Western blot - Anti-NDUFV1 antibody [HL1600] (AB308175), expandable thumbnail
  • Western blot - Anti-NDUFV1 antibody [HL1600] (AB308175), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human NDUFV1. The exact immunogen used to generate this antibody is proprietary information. Database link P49821

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Expected
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:28844695). Part of the peripheral arm of the enzyme, where the electrons from NADH are accepted by flavin mononucleotide (FMN) and then passed along a chain of iron-sulfur clusters by electron tunnelling to the final acceptor ubiquinone (PubMed:28844695). Contains FMN, which is the initial electron acceptor as well as one iron-sulfur cluster (PubMed:28844695).

Alternative names

UQOR1, NDUFV1, Complex I-51kD, NADH dehydrogenase flavoprotein 1, NADH-ubiquinone oxidoreductase 51 kDa subunit, CI-51kD

Recommended products

Rabbit Monoclonal NDUFV1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human NDUFV1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human NDUFV1. The exact immunogen used to generate this antibody is proprietary information. Database link P49821
Carrier free
Yes
Clone number
HL1600
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-NDUFV1 antibody [HL1600] (ab308175), expandable thumbnail

    Western blot - Anti-NDUFV1 antibody [HL1600] (ab308175)

    All lanes: Western blot - Anti-NDUFV1 antibody [HL1600] (ab308175) at 1/5000 dilution

    Lane 1: Wild-type (WT) 293T cell extract at 30 µg

    Lane 2: NDUFV1 knockout (KO) 293T cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 51 kDa

  • Western blot - Anti-NDUFV1 antibody [HL1600] (ab308175), expandable thumbnail

    Western blot - Anti-NDUFV1 antibody [HL1600] (ab308175)

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [HL1600] (ab308175) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-NDUFV1 antibody [HL1600] (ab308175) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 30 µg

    Lane 2: HepG2 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 50.8 kDa

  • Western blot - Anti-NDUFV1 antibody [HL1600] (ab308175), expandable thumbnail

    Western blot - Anti-NDUFV1 antibody [HL1600] (ab308175)

    Immunohistochemical analysis of paraffin-embedded rat pancreas (left) and kidney (right) tissues labeling NDUFV1 using ab308175 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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