JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308175

Anti-NDUFV1 antibody [HL1600]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal NDUFV1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human NDUFV1.

View Alternative Names

UQOR1, NDUFV1, Complex I-51kD, NADH dehydrogenase flavoprotein 1, NADH-ubiquinone oxidoreductase 51 kDa subunit, CI-51kD

3 Images
Western blot - Anti-NDUFV1 antibody [HL1600] (AB308175)
  • WB

Supplier Data

Western blot - Anti-NDUFV1 antibody [HL1600] (AB308175)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [HL1600] (ab308175) diluted at 1/1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-NDUFV1 antibody [HL1600] (ab308175) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

HepG2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50.8 kDa

false

Western blot - Anti-NDUFV1 antibody [HL1600] (AB308175)
  • WB

Supplier Data

Western blot - Anti-NDUFV1 antibody [HL1600] (AB308175)

All lanes:

Western blot - Anti-NDUFV1 antibody [HL1600] (ab308175) at 1/5000 dilution

Lane 1:

Wild-type (WT) 293T cell extract at 30 µg

Lane 2:

NDUFV1 knockout (KO) 293T cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51 kDa

false

Western blot - Anti-NDUFV1 antibody [HL1600] (AB308175)
  • WB

Supplier Data

Western blot - Anti-NDUFV1 antibody [HL1600] (AB308175)

Immunohistochemical analysis of paraffin-embedded rat pancreas (left) and kidney (right) tissues labeling NDUFV1 using ab308175 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1600

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NDUFV1. The exact immunogen used to generate this antibody is proprietary information.

P49821

style
left-column

Complementary Products

Assay Kits

AB178011

Human NADH dehydrogenase ELISA Kit (Complex I)

SimpleStep

Sandwich ELISA - Human NADH dehydrogenase ELISA Kit (Complex I) (AB178011)

  • 0
  • 0
Primary Antibodies

AB14713

Anti-NDUFA9 antibody [20C11B11B11]

KO Validated

Western blot - Anti-NDUFA9 antibody [20C11B11B11] (AB14713)

  • 4
  • 8
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

Recombinant|Lab Essentials

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
  • 52
Primary Antibodies

AB14748

Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (AB14748)

  • 5
  • 27
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFV1 also known as NADH-ubiquinone oxidoreductase 51 kDa subunit plays a mechanical role in the electron transport chain. It has a molecular mass of approximately 51 kDa. The protein is an integral part of Complex I or NADH:ubiquinone oxidoreductase located in the inner mitochondrial membrane. NDUFV1 shows expression across various tissues with higher levels found in metabolically active tissues like skeletal muscle and heart.
Biological function summary

NDUFV1 participates in oxidative phosphorylation within cells. This protein functions as a core subunit of Complex I which is the first enzyme in the mitochondrial respiratory chain. NDUFV1 has a role in transferring electrons from NADH to ubiquinone an essential step for energy production. The efficient operation of Complex I affects ATP synthesis which is necessary for many cellular processes.

Pathways

NDUFV1 is central to the energy production in mitochondria. It is important for the oxidative phosphorylation pathway and is involved in maintaining the proton gradient across the inner mitochondrial membrane. This protein closely interacts with other subunits of Complex I like NDUFS1 and has significant synergy with them in electron transfer processes. Its activity is indispensable for proper cellular respiration and energy metabolism.

NDUFV1 mutations link to mitochondrial diseases such as Leigh syndrome and some cases of Complex I deficiency. In patients with these conditions there are disruptions in ATP production due to impaired electron transport chain function. The protein may also be involved in some forms of neurodegenerative diseases where mitochondrial dysfunction plays a role. NDUFS1 and other Complex I subunits often show associations with these disorders highlighting the importance of NDUFV1 in neuromuscular and metabolic health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 28844695). Part of the peripheral arm of the enzyme, where the electrons from NADH are accepted by flavin mononucleotide (FMN) and then passed along a chain of iron-sulfur clusters by electron tunnelling to the final acceptor ubiquinone (PubMed : 28844695). Contains FMN, which is the initial electron acceptor as well as one iron-sulfur cluster (PubMed : 28844695).
See full target information NDUFV1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com