AB183717

Anti-NDUFV2 antibody [EPR15350(B)]

RabMAb
Recombinant
KO Validated
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(3 Publications)

Rabbit Recombinant Monoclonal NDUFV2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

NDUFV2, NADH-ubiquinone oxidoreductase 24 kDa subunit

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV2 antibody [EPR15350(B)] (AB183717)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV2 antibody [EPR15350(B)] (AB183717)

Immunohistochemical analysis of paraffin-embedded, Human endometrial adenocarcinoma tissue labeling NDUFV2 with ab183717 at a 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-NDUFV2 antibody [EPR15350(B)] (AB183717)

Western blot analysis on immunoprecipitation from 1) A549 cell lysate, or 2) TBS, labeling NDUFV2 using ab183717 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717)

Predicted band size: 27 kDa

Observed band size: 24 kDa

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Lab

Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (AB183717)

Lanes 1-3 : Merged signal (red and green). Green - ab183717 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.

ab183717 Anti-NDUFV2 antibody [EPR15350(B)] was shown to specifically react with NDUFV2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265619 (knockout cell lysate ab258068) was used. Wild-type and NDUFV2 knockout samples were subjected to SDS-PAGE. ab183717 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

NDUFV2 knockout HeLa cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

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Supplier Data

Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (AB183717)

All lanes:

Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (ab183717) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Ramos cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 24 kDa

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Carrier free

Anti-NDUFV2 antibody [EPR15350(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15350(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFV2 also known as NADH dehydrogenase [ubiquinone] flavoprotein 2 functions as a core subunit of complex I in the mitochondrial electron transport chain. This protein has a mass of approximately 24 kDa and plays a pivotal role in electron transfer by linking flavin mononucleotide and iron-sulfur centers. It is expressed in various tissues but shows high levels in cells with elevated energy demands such as muscle and brain tissues.

Biological function summary

NDUFV2 contributes to energy production in the cell by facilitating the transfer of electrons from NADH to the ubiquinone pool. As part of complex I which is the first enzyme complex in the respiratory electron transport chain it helps in establishing the proton gradient across the inner mitochondrial membrane. The activity of NDUFV2 is essential for ATP synthesis and its proper function ensures efficient cellular respiration.

Pathways

NDUFV2 operates in the oxidative phosphorylation pathway an important energy production route. This protein is intricately linked with other complex I subunits including NDUFS1 which work together to initiate the process of oxidative phosphorylation. Another key component in this pathway is ubiquinone which serves as the electron transfer intermediary continuing the electron passage through the chain.

Mutations in NDUFV2 have associations with neurodegenerative diseases and cardiac dysfunction. For instance patients with Leber Hereditary Optic Neuropathy often present alterations in complex I that involve NDUFV2. Additionally disruptions in its function can influence the onset of conditions such as cardiomyopathy where complex I perturbation including proteins like NDUFS1 can compromise mitochondrial energy production leading to heart muscle inefficiency.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (Probable). Parts of the peripheral arm of the enzyme, where the electrons from NADH are accepted by flavin mononucleotide (FMN) and then passed along a chain of iron-sulfur clusters by electron tunnelling to the final acceptor ubiquinone (Probable). Contains one iron-sulfur cluster (Probable).

See full target information NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 36:ar33 PubMed39878654

2025

Adaptive protein synthesis in genetic models of copper deficiency and childhood neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia R Lane,Noah E Scher,Shatabdi Bhattacharjee,Stephanie A Zlatic,Anne M Roberts,Avanti Gokhale,Kaela S Singleton,Duc M Duong,Mike McKenna,William L Liu,Alina Baiju,Felix G Rivera Moctezuma,Tommy Tran,Atit A Patel,Lauren B Clayton,Michael J Petris,Levi B Wood,Anupam Patgiri,Alysia D Vrailas-Mortimer,Daniel N Cox,Blaine R Roberts,Erica Werner,Victor Faundez

Redox biology 69:103007 PubMed38150993

2023

Inhibiting the compensatory elevation of xCT collaborates with disulfiram/copper-induced GSH consumption for cascade ferroptosis and cuproptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zhang,Chaoting Zhou,Xueying Ren,Qiangan Jing,Yan Gao,Chen Yang,Yuhuan Shen,Yi Zhou,Wanye Hu,Feifan Jin,Haifeng Xu,Lingyan Yu,Yingchao Liu,Xiangmin Tong,Yanchun Li,Ying Wang,Jing Du

Redox biology 32:101483 PubMed32169822

2020

Identification of Frataxin as a regulator of ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Du,Yi Zhou,Yanchun Li,Jun Xia,Yongjian Chen,Sufeng Chen,Xin Wang,Weidong Sun,Tongtong Wang,Xueying Ren,Xu Wang,Yihan An,Kang Lu,Wanye Hu,Siyuan Huang,Jianghui Li,Xiangmin Tong,Ying Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NDUFV2 antibody [EPR15350(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV2 antibody [EPR15350(B)] (AB183717)

Immunoprecipitation - Anti-NDUFV2 antibody [EPR15350(B)] (AB183717)

Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (AB183717)

Western blot - Anti-NDUFV2 antibody [EPR15350(B)] (AB183717)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise