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AB183715

Anti-NDUFV2 antibody [EPR15351(B)]

5

(1 Review)

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(12 Publications)

Rabbit Recombinant Monoclonal NDUFV2 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications.

View Alternative Names

NDUFV2, NADH-ubiquinone oxidoreductase 24 kDa subunit

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)

Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling NDUFV2 with ab183715 at 1/1000. Cells were fixed with 100% Methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

Control : PBS only.
Nuclear counter stain : DAPI.

Flow Cytometry (Intracellular) - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)

Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling NDUFV2 with purified ab183715 at 1/80 dilution (10ug/mL) (red). Cells were fixed with 80% methanol and permeabilised using 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black) (ab172730). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)

Immunohistochemical analysis of paraffin-embedded, Human hepatocellular carcinoma tissue labeling NDUFV2 with ab183715 at a 1/500 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)
  • IP

Supplier Data

Immunoprecipitation - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)

Western blot analysis on immunoprecipitation from 1) Human fetal heart lysate, or 2) TBS, labeling NDUFV2 using ab183715 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-NDUFV2 antibody [EPR15351(B)] (ab183715)

Predicted band size: 27 kDa

Observed band size: 24 kDa

true

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)
  • WB

Supplier Data

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)

All lanes:

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] (ab183715) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Ramos cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 24 kDa

true

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)
  • WB

Lab

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)

Lanes 1-3 : Merged signal (red and green). Green - ab183715 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.

ab183715 Anti-NDUFV2 antibody [EPR15351(B)] was shown to specifically react with NDUFV2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265619 (knockout cell lysate ab258068) was used. Wild-type and NDUFV2 knockout samples were subjected to SDS-PAGE. ab183715 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] (ab183715) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

NDUFV2 knockout HeLa cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)
  • WB

Supplier Data

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] (AB183715)

All lanes:

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] (ab183715) at 1/20000 dilution

All lanes:

Human fetal heart lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 24 kDa

true

  • Carrier free

    Anti-NDUFV2 antibody [EPR15351(B)] - BSA and Azide free

  • HRP

    HRP Anti-NDUFV2 antibody [EPR15351(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15351(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, Flow Cyt (Intra), WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFV2 antibody [EPR15350(B)] (AB183717)

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFV2 also known as NADH dehydrogenase [ubiquinone] flavoprotein 2 functions as a core subunit of complex I in the mitochondrial electron transport chain. This protein has a mass of approximately 24 kDa and plays a pivotal role in electron transfer by linking flavin mononucleotide and iron-sulfur centers. It is expressed in various tissues but shows high levels in cells with elevated energy demands such as muscle and brain tissues.
Biological function summary

NDUFV2 contributes to energy production in the cell by facilitating the transfer of electrons from NADH to the ubiquinone pool. As part of complex I which is the first enzyme complex in the respiratory electron transport chain it helps in establishing the proton gradient across the inner mitochondrial membrane. The activity of NDUFV2 is essential for ATP synthesis and its proper function ensures efficient cellular respiration.

Pathways

NDUFV2 operates in the oxidative phosphorylation pathway an important energy production route. This protein is intricately linked with other complex I subunits including NDUFS1 which work together to initiate the process of oxidative phosphorylation. Another key component in this pathway is ubiquinone which serves as the electron transfer intermediary continuing the electron passage through the chain.

Mutations in NDUFV2 have associations with neurodegenerative diseases and cardiac dysfunction. For instance patients with Leber Hereditary Optic Neuropathy often present alterations in complex I that involve NDUFV2. Additionally disruptions in its function can influence the onset of conditions such as cardiomyopathy where complex I perturbation including proteins like NDUFS1 can compromise mitochondrial energy production leading to heart muscle inefficiency.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (Probable). Parts of the peripheral arm of the enzyme, where the electrons from NADH are accepted by flavin mononucleotide (FMN) and then passed along a chain of iron-sulfur clusters by electron tunnelling to the final acceptor ubiquinone (Probable). Contains one iron-sulfur cluster (Probable).
See full target information NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108433 PubMed40120684

2025

HS remodels mitochondrial ultrastructure and destabilizes respiratory supercomplexes.

Applications

Unspecified application

Species

Unspecified reactive species

David A Hanna,Brandon Chen,Yatrik M Shah,Oleh Khalimonchuk,Brian Cunniff,Ruma Banerjee

Redox biology 65:102812 PubMed37451140

2023

PHB2 ameliorates Doxorubicin-induced cardiomyopathy through interaction with NDUFV2 and restoration of mitochondrial complex I function.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjie Yang,Miyesaier Abudureyimu,Xiang Wang,Yuan Zhou,Yingmei Zhang,Jun Ren

Cardiovascular research 119:2213-2229 PubMed37395010

2023

Tissue-specific differences in the assembly of mitochondrial Complex I are revealed by a novel ENU mutation in ECSIT.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Nicol,Sara Falcone,Andrew Blease,Pratik Vikhe,Gabriele Civiletto,Saleh Salman Omairi,Carlo Viscomi,Ketan Patel,Paul K Potter

Bioengineered 13:13089-13107 PubMed35615982

2022

CDC25C is a prognostic biomarker and correlated with mitochondrial homeostasis in pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoting Zhou,Luyang Wang,Wanye Hu,Lusheng Tang,Ping Zhang,Yan Gao,Jing Du,Yanchun Li,Ying Wang

Cancers 14: PubMed35267509

2022

Broad Transcriptomic Impact of Sorafenib and Its Relation to the Antitumoral Properties in Liver Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Contreras,Alfonso Rodríguez-Gil,Jordi Muntané,Jesús de la Cruz

Neural regeneration research 16:2438-2445 PubMed33907032

2021

Protein profiling identified mitochondrial dysfunction and synaptic abnormalities after dexamethasone intervention in rats with traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Niu,Bin Zhang,Jie Feng,Xiang Mao,Xiao-Jian Xu,Jin-Qian Dong,Bai-Yun Liu

Journal of neurochemistry 157:550-560 PubMed33305362

2020

Nicotinamide ameliorates energy deficiency and improves retinal function in Cav-1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhen Tang,Wangyi Fang,Zebin Xiao,Maomao Song,Dongli Zhuang,Binze Han,Jihong Wu,Xinghuai Sun

Biochemical and biophysical research communications 534:877-884 PubMed33162029

2020

Sorafenib induces mitochondrial dysfunction and exhibits synergistic effect with cysteine depletion by promoting HCC cells ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchun Li,Jun Xia,Fangchun Shao,Yan Zhou,Jiaqi Yu,Hengyu Wu,Jing Du,Xueying Ren

Nature 583:603-608 PubMed32641832

2020

Glucose metabolism links astroglial mitochondria to cannabinoid effects.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Jimenez-Blasco,Arnau Busquets-Garcia,Etienne Hebert-Chatelain,Roman Serrat,Carlos Vicente-Gutierrez,Christina Ioannidou,Paula Gómez-Sotres,Irene Lopez-Fabuel,Monica Resch-Beusher,Eva Resel,Dorian Arnouil,Dave Saraswat,Marjorie Varilh,Astrid Cannich,Francisca Julio-Kalajzic,Itziar Bonilla-Del Río,Angeles Almeida,Nagore Puente,Svein Achicallende,Maria-Luz Lopez-Rodriguez,Charlotte Jollé,Nicole Déglon,Luc Pellerin,Charlène Josephine,Gilles Bonvento,Aude Panatier,Beat Lutz,Pier-Vincenzo Piazza,Manuel Guzmán,Luigi Bellocchio,Anne-Karine Bouzier-Sore,Pedro Grandes,Juan P Bolaños,Giovanni Marsicano

Cell reports 28:218-230.e7 PubMed31269442

2019

Nitric Oxide Modulates Metabolic Remodeling in Inflammatory Macrophages through TCA Cycle Regulation and Itaconate Accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jade D Bailey,Marina Diotallevi,Thomas Nicol,Eileen McNeill,Andrew Shaw,Surawee Chuaiphichai,Ashley Hale,Anna Starr,Manasi Nandi,Elena Stylianou,Helen McShane,Simon Davis,Roman Fischer,Benedikt M Kessler,James McCullagh,Keith M Channon,Mark J Crabtree
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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