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AB250689

Anti-NDUFV2 antibody [EPR15351(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NDUFV2 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NDUFV2, NADH-ubiquinone oxidoreductase 24 kDa subunit

1 Images
Western blot - Anti-NDUFV2 antibody [EPR15351(B)] - BSA and Azide free (AB250689)
  • WB

Lab

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] - BSA and Azide free (AB250689)

This data was developed using the same antibody clone in a different buffer formulation (ab183715).

Lanes 1-3 : Merged signal (red and green). Green - ab183715 observed at 27 kDa. Red - loading control ab7291 observed at 50 kDa.

ab183715 Anti-NDUFV2 antibody [EPR15351(B)] was shown to specifically react with NDUFV2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265619 (knockout cell lysate ab258068) was used. Wild-type and NDUFV2 knockout samples were subjected to SDS-PAGE. ab183715 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NDUFV2 antibody [EPR15351(B)] (<a href='/en-us/products/primary-antibodies/ndufv2-antibody-epr15351b-ab183715'>ab183715</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

NDUFV2 knockout HeLa cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

  • Unconjugated

    Anti-NDUFV2 antibody [EPR15351(B)]

  • HRP

    HRP Anti-NDUFV2 antibody [EPR15351(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15351(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, ICC/IF, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250689 is the carrier-free version of ab183715.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NDUFV2 also known as NADH dehydrogenase [ubiquinone] flavoprotein 2 functions as a core subunit of complex I in the mitochondrial electron transport chain. This protein has a mass of approximately 24 kDa and plays a pivotal role in electron transfer by linking flavin mononucleotide and iron-sulfur centers. It is expressed in various tissues but shows high levels in cells with elevated energy demands such as muscle and brain tissues.
Biological function summary

NDUFV2 contributes to energy production in the cell by facilitating the transfer of electrons from NADH to the ubiquinone pool. As part of complex I which is the first enzyme complex in the respiratory electron transport chain it helps in establishing the proton gradient across the inner mitochondrial membrane. The activity of NDUFV2 is essential for ATP synthesis and its proper function ensures efficient cellular respiration.

Pathways

NDUFV2 operates in the oxidative phosphorylation pathway an important energy production route. This protein is intricately linked with other complex I subunits including NDUFS1 which work together to initiate the process of oxidative phosphorylation. Another key component in this pathway is ubiquinone which serves as the electron transfer intermediary continuing the electron passage through the chain.

Mutations in NDUFV2 have associations with neurodegenerative diseases and cardiac dysfunction. For instance patients with Leber Hereditary Optic Neuropathy often present alterations in complex I that involve NDUFV2. Additionally disruptions in its function can influence the onset of conditions such as cardiomyopathy where complex I perturbation including proteins like NDUFS1 can compromise mitochondrial energy production leading to heart muscle inefficiency.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (Probable). Parts of the peripheral arm of the enzyme, where the electrons from NADH are accepted by flavin mononucleotide (FMN) and then passed along a chain of iron-sulfur clusters by electron tunnelling to the final acceptor ubiquinone (Probable). Contains one iron-sulfur cluster (Probable).
See full target information NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed31094704

2019

Protective role of B cells in sterile particulate-induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shaikh M Atif,Douglas G Mack,Amy S McKee,Javier Rangel-Moreno,Allison K Martin,Andrew Getahun,Lisa A Maier,John C Cambier,Rubin Tuder,Andrew P Fontenot
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com