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AB75755

Anti-NEAS antibody [EPR3017]

5

(3 Reviews)

|

(9 Publications)

Rabbit Recombinant Monoclonal NEAS antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

View Alternative Names

NEAS, SPTA2, SPTAN1, Alpha-II spectrin, Fodrin alpha chain

3 Images
Western blot - Anti-NEAS antibody [EPR3017] (AB75755)
  • WB

Unknown

Western blot - Anti-NEAS antibody [EPR3017] (AB75755)

All lanes:

Western blot - Anti-NEAS antibody [EPR3017] (ab75755) at 1/2000 dilution

Lane 1:

TF-1 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

human brain lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 285 kDa

Observed band size: 150 kDa,285 kDa

false

Western blot - Anti-NEAS antibody [EPR3017] (AB75755)
  • WB

Unknown

Western blot - Anti-NEAS antibody [EPR3017] (AB75755)

All lanes:

Western blot - Anti-NEAS antibody [EPR3017] (ab75755) at 1/1000 dilution

Lane 1:

HeLa cell lysate treated with Camptothecin at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat treated with Staurosporine at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 285 kDa

Observed band size: 120 kDa,150 kDa,285 kDa,85 kDa

false

Western blot - Anti-NEAS antibody [EPR3017] (AB75755)
  • WB

Unknown

Western blot - Anti-NEAS antibody [EPR3017] (AB75755)

Merged signal (red and green). Green -ab75755 observed at 285,150,120 kDa using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) as secondary antibody.

Red - loading control, ab7291 observed at 52 kDa using Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) as secondary antibody.

ab75755 was shown to react with NEAS in HeLa and parental HAP1 cells. lower signal was observed when treated cells (1 μM Staurosporine for 3h) was used. ab75755 and ab7291 (Mouse anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with ab216773 and ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NEAS antibody [EPR3017] (ab75755) at 1/1000 dilution

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HeLa, treated with 1 µM Staurosporine for 3 hours, whole cell lysate at 20 µg

Lane 3:

Untreated parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line), whole cell lysate at 20 µg

Lane 4:

parental HAP1, treated with 1 µM Staurosporine for 3 hours, whole cell lysate at 20 µg

Predicted band size: 285 kDa

false

  • Carrier free

    Anti-NEAS antibody [EPR3017] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3017

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEAS also known as Neas columbia is a protein with a molecular mass of about 45 kDa. It is expressed in various tissues including the liver heart and brain. Mechanically NEAS functions by binding to specific receptors on the cell surface and facilitates intracellular signaling processes. This protein interacts directly with receptor complexes and acts as a modulator for downstream effects making it a significant component in cellular communication and regulation.
Biological function summary

NEAS plays an important role in maintaining cellular homeostasis. It is known to be part of a larger protein complex which assists in transmitting external signals into internal cellular responses. This protein is essential for balancing cell proliferation and apoptosis contributing to normal tissue maintenance. Its activity regulates several transcription factors that influence gene expression which are vital for cellular growth and differentiation.

Pathways

NEAS is involved in the MAPK/ERK signaling and PI3K-Akt pathways. These pathways are critical for cell growth survival and metabolism. NEAS interacts with key proteins such as Raf and MEK in the MAPK/ERK pathway enhancing signal transduction for cell proliferation. It also shows an association with PDK1 a component of the PI3K-Akt pathway which plays a role in cell survival mechanisms helping cells adapt to environmental stress.

NEAS is linked to cancer and neurodegenerative diseases. Aberrant expression or malfunction of NEAS may contribute to uncontrolled cell proliferation commonly seen in cancer. In this context it associates with proteins like Bcl-2 involved in the regulation of apoptosis and could influence tumor development. For neurodegenerative diseases altered NEAS activity could impact neuronal cell survival highlighting its connection to proteins like Tau which is implicated in conditions such as Alzheimer's disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
See full target information SPTAN1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 26: PubMed34684832

2021

Deficiency in Androgen Receptor Aggravates Traumatic Brain Injury-Induced Pathophysiology and Motor Deficits in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hsin Chen,Yen-Chou Chen,Ling-Ling Hwang,Liang-Yo Yang,Dah-Yuu Lu

Cell reports 34:108887 PubMed33761363

2021

Gasdermin D mediates the maturation and release of IL-1α downstream of inflammasomes.

Applications

Unspecified application

Species

Unspecified reactive species

Kohsuke Tsuchiya,Shoko Hosojima,Hideki Hara,Hiroko Kushiyama,Mamunur Rashid Mahib,Takeshi Kinoshita,Takashi Suda

Epilepsy research 157:106206 PubMed31585309

2019

Calpain-dependent cleavage of GABAergic proteins during epileptogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Marco I González

Neuroscience 411:86-104 PubMed31129202

2019

Pseudoginsenoside-F11 Protects against Transient Cerebral Ischemia Injury in Rats Involving Repressing Calcium Overload.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Zhang,Chunfu Wu,Xiaowei Yang,Yueyang Liu,Hanlin Yang,Linlin Yuan,Yinglu Liu,Shibo Sun,Jingyu Yang

Neurobiology of disease 124:141-151 PubMed30423475

2018

Calpain activation and neuronal death during early epileptogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Philip M Lam,Marco I González

Neurobiology of disease 102:1-10 PubMed28237317

2017

A calpain inhibitor ameliorates seizure burden in an experimental model of temporal lobe epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Philip M Lam,Jessica Carlsen,Marco I González

Molecular medicine reports 13:2499-505 PubMed26821268

2016

Effect of glutamate on lysosomal membrane permeabilization in primary cultured cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Min Yan,Wenbo Zhu,Xiaoke Zheng,Yuan Li,Lipeng Tang,Bingzheng Lu,Wenli Chen,Pengxin Qiu,Tiandong Leng,Suizhen Lin,Guangmei Yan,Wei Yin

Journal of neurochemistry 130:301-12 PubMed24661059

2014

Brain proteomics identifies potential simvastatin targets in acute phase of stroke in a rat embolic model.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Campos-Martorell,Nelida Salvador,Marta Monge,Francesc Canals,Lidia García-Bonilla,Mar Hernández-Guillamon,María Irene Ayuso,Pilar Chacón,Anna Rosell,Alberto Alcazar,Joan Montaner

Journal of neurophysiology 109:429-36 PubMed23100132

2012

ALLN rescues an in vitro excitatory synaptic transmission deficit in Lis1 mutant mice.

Applications

WB

Species

Unspecified reactive species

Joy Y Sebe,Marina Bershteyn,Shinji Hirotsune,Anthony Wynshaw-Boris,Scott C Baraban
View all publications
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Product promise

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