Rabbit Recombinant Monoclonal NEAS antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
Images
![Western blot - Anti-NEAS antibody [EPR3017] (AB75755), expandable thumbnail](https://content.abcam.com/products/images/neas-antibody-epr3017-ab75755--western-blot-img2816.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NEAS antibody [EPR3017] (AB75755)")
Publications
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- Molecules (Basel, Switzerland) 26:2021Deficiency in Androgen Receptor Aggravates Traumatic Brain Injury-Induced Pathophysiology and Motor Deficits in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Hsin Chen,Yen-Chou Chen,Ling-Ling Hwang,Liang-Yo Yang,Dah-Yuu LuPubMed 34684832
- Cell reports 34:1088872021Gasdermin D mediates the maturation and release of IL-1α downstream of inflammasomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKohsuke Tsuchiya,Shoko Hosojima,Hideki Hara,Hiroko Kushiyama,Mamunur Rashid Mahib,Takeshi Kinoshita,Takashi SudaPubMed 33761363
- Epilepsy research 157:1062062019Calpain-dependent cleavage of GABAergic proteins during epileptogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco I GonzálezPubMed 31585309
- Neuroscience 411:86-1042019Pseudoginsenoside-F11 Protects against Transient Cerebral Ischemia Injury in Rats Involving Repressing Calcium Overload.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianyu Zhang et. al.PubMed 31129202
- Neurobiology of disease 124:141-1512018Calpain activation and neuronal death during early epileptogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhilip M Lam,Marco I GonzálezPubMed 30423475
- Neurobiology of disease 102:1-102017A calpain inhibitor ameliorates seizure burden in an experimental model of temporal lobe epilepsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhilip M Lam et. al.PubMed 28237317
- Molecular medicine reports 13:2499-5052016Effect of glutamate on lysosomal membrane permeabilization in primary cultured cortical neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Yan et. al.PubMed 26821268
- Journal of neurochemistry 130:301-122014Brain proteomics identifies potential simvastatin targets in acute phase of stroke in a rat embolic model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMireia Campos-Martorell et. al.PubMed 24661059
- Journal of neurophysiology 109:429-362012ALLN rescues an in vitro excitatory synaptic transmission deficit in Lis1 mutant mice.Applications:WBReactive species:Unspecified reactive speciesJoy Y Sebe,Marina Bershteyn,Shinji Hirotsune,Anthony Wynshaw-Boris,Scott C BarabanPubMed 23100132
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% PBS, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Expected | Not recommended |
| Rat | Not recommended | Not recommended | Expected | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2000 | Notes - |
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Alternative names
NEAS, SPTA2, SPTAN1, Alpha-II spectrin, Fodrin alpha chain
Recommended products
Rabbit Recombinant Monoclonal NEAS antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% PBS, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3017
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NEAS also known as Neas columbia is a protein with a molecular mass of about 45 kDa. It is expressed in various tissues including the liver heart and brain. Mechanically NEAS functions by binding to specific receptors on the cell surface and facilitates intracellular signaling processes. This protein interacts directly with receptor complexes and acts as a modulator for downstream effects making it a significant component in cellular communication and regulation.
- Biological function summary
NEAS plays an important role in maintaining cellular homeostasis. It is known to be part of a larger protein complex which assists in transmitting external signals into internal cellular responses. This protein is essential for balancing cell proliferation and apoptosis contributing to normal tissue maintenance. Its activity regulates several transcription factors that influence gene expression which are vital for cellular growth and differentiation.
- Pathways
NEAS is involved in the MAPK/ERK signaling and PI3K-Akt pathways. These pathways are critical for cell growth survival and metabolism. NEAS interacts with key proteins such as Raf and MEK in the MAPK/ERK pathway enhancing signal transduction for cell proliferation. It also shows an association with PDK1 a component of the PI3K-Akt pathway which plays a role in cell survival mechanisms helping cells adapt to environmental stress.
- Associated diseases and disorders
NEAS is linked to cancer and neurodegenerative diseases. Aberrant expression or malfunction of NEAS may contribute to uncontrolled cell proliferation commonly seen in cancer. In this context it associates with proteins like Bcl-2 involved in the regulation of apoptosis and could influence tumor development. For neurodegenerative diseases altered NEAS activity could impact neuronal cell survival highlighting its connection to proteins like Tau which is implicated in conditions such as Alzheimer's disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-NEAS antibody [EPR3017] (ab75755), expandable thumbnail](https://content.abcam.com/products/images/neas-antibody-epr3017-ab75755--western-blot-img2816.png "Western blot - Anti-NEAS antibody [EPR3017] (ab75755)")
Western blot - Anti-NEAS antibody [EPR3017] (ab75755)
Merged signal (red and green). Green -ab75755 observed at 285,150,120 kDa using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) as secondary antibody.
Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 52 kDa using Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) as secondary antibody.
ab75755 was shown to react with NEAS in HeLa and parental HAP1 cells. lower signal was observed when treated cells (1 μM Staurosporine for 3h) was used. ab75755 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-NEAS antibody [EPR3017] (ab75755) at 1/1000 dilution
Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: HeLa, treated with 1 µM Staurosporine for 3 hours, whole cell lysate at 20 µg
Lane 3: Untreated parental HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line), whole cell lysate at 20 µg
Lane 4: parental HAP1, treated with 1 µM Staurosporine for 3 hours, whole cell lysate at 20 µg
Predicted band size: 285 kDa
![Western blot - Anti-NEAS antibody [EPR3017] (ab75755), expandable thumbnail](https://content.abcam.com/products/images/neas-antibody-epr3017-ab75755--western-blot-img138777.gif "Western blot - Anti-NEAS antibody [EPR3017] (ab75755)")
Western blot - Anti-NEAS antibody [EPR3017] (ab75755)
All lanes: Western blot - Anti-NEAS antibody [EPR3017] (ab75755) at 1/1000 dilution
Lane 1: HeLa cell lysate treated with Camptothecin at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: Jurkat treated with Staurosporine at 10 µg
Lane 4: Jurkat cell lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 120 kDa, 150 kDa, 285 kDa, 85 kDa
![Western blot - Anti-NEAS antibody [EPR3017] (ab75755), expandable thumbnail](https://content.abcam.com/products/images/neas-antibody-epr3017-ab75755--western-blot-img137045.gif "Western blot - Anti-NEAS antibody [EPR3017] (ab75755)")
Western blot - Anti-NEAS antibody [EPR3017] (ab75755)
All lanes: Western blot - Anti-NEAS antibody [EPR3017] (ab75755) at 1/2000 dilution
Lane 1: TF-1 cell lysate at 10 µg
Lane 2: SH-SY5Y cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: human brain lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 150 kDa, 285 kDa
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