Rabbit Recombinant Monoclonal NEAS antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (AB239863), expandable thumbnail](https://content.abcam.com/products/images/neas-antibody-epr3017-bsa-and-azide-free-ab239863--western-blot-img138777.gif "Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (AB239863)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Expected | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Alternative names
NEAS, SPTA2, SPTAN1, Alpha-II spectrin, Fodrin alpha chain
Recommended products
Rabbit Recombinant Monoclonal NEAS antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3017
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239863 is the carrier-free version of Anti-NEAS antibody [EPR3017] ab75755.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NEAS also known as Neas columbia is a protein with a molecular mass of about 45 kDa. It is expressed in various tissues including the liver heart and brain. Mechanically NEAS functions by binding to specific receptors on the cell surface and facilitates intracellular signaling processes. This protein interacts directly with receptor complexes and acts as a modulator for downstream effects making it a significant component in cellular communication and regulation.
- Biological function summary
NEAS plays an important role in maintaining cellular homeostasis. It is known to be part of a larger protein complex which assists in transmitting external signals into internal cellular responses. This protein is essential for balancing cell proliferation and apoptosis contributing to normal tissue maintenance. Its activity regulates several transcription factors that influence gene expression which are vital for cellular growth and differentiation.
- Pathways
NEAS is involved in the MAPK/ERK signaling and PI3K-Akt pathways. These pathways are critical for cell growth survival and metabolism. NEAS interacts with key proteins such as Raf and MEK in the MAPK/ERK pathway enhancing signal transduction for cell proliferation. It also shows an association with PDK1 a component of the PI3K-Akt pathway which plays a role in cell survival mechanisms helping cells adapt to environmental stress.
- Associated diseases and disorders
NEAS is linked to cancer and neurodegenerative diseases. Aberrant expression or malfunction of NEAS may contribute to uncontrolled cell proliferation commonly seen in cancer. In this context it associates with proteins like Bcl-2 involved in the regulation of apoptosis and could influence tumor development. For neurodegenerative diseases altered NEAS activity could impact neuronal cell survival highlighting its connection to proteins like Tau which is implicated in conditions such as Alzheimer's disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863)
This data was developed using Anti-NEAS antibody [EPR3017] ab75755, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-NEAS antibody [EPR3017] (Anti-NEAS antibody [EPR3017] ab75755) at 1/1000 dilution
Lane 1: HeLa cell lysate treated with Camptothecin at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: Jurkat treated with Staurosporine at 10 µg
Lane 4: Jurkat cell lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 120 kDa, 150 kDa, 285 kDa, 85 kDa
![Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863), expandable thumbnail](https://content.abcam.com/products/images/neas-antibody-epr3017-bsa-and-azide-free-ab239863--western-blot-img137045.gif "Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863)")
Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (ab239863)
This data was developed using Anti-NEAS antibody [EPR3017] ab75755, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-NEAS antibody [EPR3017] (Anti-NEAS antibody [EPR3017] ab75755) at 1/2000 dilution
Lane 1: TF-1 cell lysate at 10 µg
Lane 2: SH-SY5Y cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: human brain lysate at 10 µg
SecondaryAll lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 150 kDa, 285 kDa
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