AB239863

Anti-NEAS antibody [EPR3017] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal NEAS antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

NEAS, SPTA2, SPTAN1, Alpha-II spectrin, Fodrin alpha chain

2 Images

Unknown

Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (AB239863)

This data was developed using ab75755, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NEAS antibody [EPR3017] (<a href='/en-us/products/primary-antibodies/neas-antibody-epr3017-ab75755'>ab75755</a>) at 1/2000 dilution

Lane 1:

TF-1 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

human brain lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 285 kDa

Observed band size: 150 kDa,285 kDa

false

Unknown

Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (AB239863)

This data was developed using ab75755, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NEAS antibody [EPR3017] (<a href='/en-us/products/primary-antibodies/neas-antibody-epr3017-ab75755'>ab75755</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate treated with Camptothecin at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat treated with Staurosporine at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 285 kDa

Observed band size: 120 kDa,150 kDa,285 kDa,85 kDa

false

Unconjugated

Anti-NEAS antibody [EPR3017]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3017

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab239863 is the carrier-free version of ab75755.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEAS also known as Neas columbia is a protein with a molecular mass of about 45 kDa. It is expressed in various tissues including the liver heart and brain. Mechanically NEAS functions by binding to specific receptors on the cell surface and facilitates intracellular signaling processes. This protein interacts directly with receptor complexes and acts as a modulator for downstream effects making it a significant component in cellular communication and regulation.

Biological function summary

NEAS plays an important role in maintaining cellular homeostasis. It is known to be part of a larger protein complex which assists in transmitting external signals into internal cellular responses. This protein is essential for balancing cell proliferation and apoptosis contributing to normal tissue maintenance. Its activity regulates several transcription factors that influence gene expression which are vital for cellular growth and differentiation.

Pathways

NEAS is involved in the MAPK/ERK signaling and PI3K-Akt pathways. These pathways are critical for cell growth survival and metabolism. NEAS interacts with key proteins such as Raf and MEK in the MAPK/ERK pathway enhancing signal transduction for cell proliferation. It also shows an association with PDK1 a component of the PI3K-Akt pathway which plays a role in cell survival mechanisms helping cells adapt to environmental stress.

NEAS is linked to cancer and neurodegenerative diseases. Aberrant expression or malfunction of NEAS may contribute to uncontrolled cell proliferation commonly seen in cancer. In this context it associates with proteins like Bcl-2 involved in the regulation of apoptosis and could influence tumor development. For neurodegenerative diseases altered NEAS activity could impact neuronal cell survival highlighting its connection to proteins like Tau which is implicated in conditions such as Alzheimer's disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

See full target information SPTAN1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International archives of allergy and immunology 182:1245-1254 PubMed34428765

2021

Celastrol Exerts Cardioprotective Effect in Rheumatoid Arthritis by Inhibiting TLR2/HMGB1 Signaling Pathway-Mediated Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Lu,Sha Gong,Xiaojun Wang,Nan Hu,Dan Pu,Jing Zhang,Yanhua Wang,Jing Luo,Qi An,Bomiao Ju,Lan He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NEAS antibody [EPR3017] - BSA and Azide free

Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (AB239863)

Western blot - Anti-NEAS antibody [EPR3017] - BSA and Azide free (AB239863)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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