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AB224450

Anti-NECAB1 antibody

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(1 Publication)

Rabbit Polyclonal NECAB1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NECAB1 aa 100-200.

View Alternative Names

EFCBP1, NECAB1, N-terminal EF-hand calcium-binding protein 1, EF-hand calcium-binding protein 1, Neuronal calcium-binding protein 1

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)

Paraffin-embedded human pancreas stained for NECAB1 using ab224450 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)

Paraffin-embedded human colon stained for NECAB1 using ab224450 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)

Paraffin-embedded human cerebral cortex stained for NECAB1 using ab224450 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)

Paraffin-embedded human liver stained for NECAB1 using ab224450 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)

Paraffin embedded mouse parietal association cortex tissue stained for NECAB1 with ab224450 (1/1000 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)

Paraffin embedded mouse hippocampus tissue stained for NECAB1 with ab224450 (1/1000 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)

Paraffin embedded mouse superior colliculi tissue stained for NECAB1 with ab224450 (1/1000 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB1 antibody (AB224450)

Paraffin embedded mouse vestibular nucleus tissue stained for NECAB1 with ab224450 (1/1000 dilution) in immunohistochemical analysis.

Western blot - Anti-NECAB1 antibody (AB224450)
  • WB

Supplier Data

Western blot - Anti-NECAB1 antibody (AB224450)

All lanes:

Western blot - Anti-NECAB1 antibody (ab224450) at 1/100 dilution

All lanes:

Mouse cerebral cortex tissue lysate

Predicted band size: 41 kDa

false

Western blot - Anti-NECAB1 antibody (AB224450)
  • WB

Supplier Data

Western blot - Anti-NECAB1 antibody (AB224450)

All lanes:

Western blot - Anti-NECAB1 antibody (ab224450) at 1/100 dilution

All lanes:

Human cerebral cortex tissue lysate

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NECAB1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q8N987

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NECAB1 also known as N-terminal EF-hand calcium-binding protein 1 is a protein that binds calcium ions and has a molecular mass of around 40 kDa. This protein commonly expresses in the brain with high levels in regions such as the cerebral cortex and cerebellum. Its expression pattern suggests its involvement in neural function and synaptic processes. NECAB1 undergoes calcium-dependent conformational changes affecting its interaction with other proteins which positions it as a significant mediator in signaling pathways involving neural activity.
Biological function summary

NECAB1 serves as a calcium sensor and modulator within the neural network impacting synaptic transmission. It forms part of a complex with other proteins orchestrating intracellular calcium dynamics and synapse plasticity. By interacting with synaptic vesicle-associated proteins NECAB1 contributes to the regulation of neurotransmitter release showcasing its role in maintaining neuronal communication and plasticity which are critical for cognitive functions and neural circuit adaptability.

Pathways

NECAB1 is involved in neurotransmitter release and calcium signaling pathways. It interacts closely with proteins like calmodulin and synaptotagmins which are essential in the calcium-dependent release of neurotransmitters. These interactions facilitate the regulation of synaptic vesicle dynamics and neurotransmitter exocytosis. Through these pathways NECAB1 plays a part in synaptic plasticity and long-term potentiation processes that underline learning and memory.

NECAB1 associates with neurodegenerative disorders such as Alzheimer's disease and schizophrenia. Studies show alterations in NECAB1 expression levels and function in these conditions implicating dysfunctional calcium signaling and synaptic processes. It connects with proteins like tau in Alzheimer's disease where disturbances in calcium homeostasis and synaptic integrity contribute to disease pathology. Understanding NECAB1's involvement could offer insights into therapeutic targets for modulating synaptic function and neuroprotection.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information NECAB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 39:877-887 PubMed37750664

2023

Selenomethionine suppresses head and neck squamous cell carcinoma progression through TopBP1/ATR and TCAB1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Xiaodong Wei,Jiwu Li
View all publications
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Product promise

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