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AB211464

Anti-NECAB2 antibody [CL0524]

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(2 Publications)

Mouse Monoclonal NECAB2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NECAB2 aa 200-400.

View Alternative Names

EFCBP2, NECAB2, N-terminal EF-hand calcium-binding protein 2, EF-hand calcium-binding protein 2, Neuronal calcium-binding protein 2, Synaptotagmin-interacting protein 2, Stip-2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB2 antibody [CL0524] (AB211464)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB2 antibody [CL0524] (AB211464)

Immunohistochemical analysis of human colon tissue labeling NECAB2 with ab211464 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB2 antibody [CL0524] (AB211464)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB2 antibody [CL0524] (AB211464)

Immunohistochemical analysis of human cerebral cortex tissue labeling NECAB2 with ab211464 at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB2 antibody [CL0524] (AB211464)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NECAB2 antibody [CL0524] (AB211464)

Negative control : Immunohistochemical analysis of human liver tissue labeling NECAB2 with ab211464 at 1/500 dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0524

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NECAB2 aa 200-400. The exact immunogen used to generate this antibody is proprietary information.

Q7Z6G3

Epitope

This antibody binds to an epitope located within the peptide sequence IGRLESKALWFDLQQ (aa 259-273).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NECAB2 also known as N-terminal EF-hand calcium-binding protein 2 binds calcium ions through EF-hand motifs. This protein belongs to a family of EF-hand containing proteins involved in calcium signaling. It has a molecular weight around 45 kDa. NECAB2 is expressed mainly in the nervous system particularly in the brain. Studies show its expression in neurons suggesting a role in synaptic function.
Biological function summary

NECAB2 is involved in calcium-dependent signaling pathways. It associates with other proteins to form complexes impacting cellular processes like neurotransmitter release. NECAB2's role suggests it helps modulate synaptic activity and plasticity due to its involvement in regulating intracellular calcium levels. Its interactions in synaptic regions underline its importance in neural communication.

Pathways

NECAB2 participates in calcium signaling and synaptic signaling pathways. In calcium signaling NECAB2 couples with other proteins like calmodulin affecting intracellular calcium concentrations. In synaptic signaling its interaction with proteins like synaptotagmins impacts neurotransmitter release and synapse maintenance. These pathways highlight NECAB2's role in maintaining neural communication and function.

NECAB2 links to neurological conditions such as autism spectrum disorders and schizophrenia. Studies show altered NECAB2 expression in these conditions possibly disrupting normal synaptic function. Its interactions with proteins like neurexins and neuroligins which are implicated in synapse formation and function might contribute to the pathophysiology of these disorders. Understanding NECAB2 in this context could offer insights into therapeutic targets for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a signaling scaffold protein that senses intracellular calcium. Can modulate ligand-induced internalization of ADORA2A and coupling efficiency of mGluR5/GRM5; for both receptors may regulate signaling activity such as promoting MAPK1/3 (ERK1/2) activation.
See full target information NECAB2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:6264 PubMed39048565

2024

Pannexin-1 channel inhibition alleviates opioid withdrawal in rodents by modulating locus coeruleus to spinal cord circuitry.

Applications

Unspecified application

Species

Unspecified reactive species

Charlie H T Kwok,Erika K Harding,Nicole E Burma,Tamara Markovic,Nicolas Massaly,Nynke J van den Hoogen,Sierra Stokes-Heck,Eder Gambeta,Kristina Komarek,Hye Jean Yoon,Kathleen E Navis,Brendan B McAllister,Julia Canet-Pons,Churmy Fan,Rebecca Dalgarno,Evgueni Gorobets,James W Papatzimas,Zizhen Zhang,Yuta Kohro,Connor L Anderson,Roger J Thompson,Darren J Derksen,Jose A Morón,Gerald W Zamponi,Tuan Trang

Journal of molecular neuroscience : MN 71:55-65 PubMed32557241

2020

TRPV1, Targeted by miR-338-3p, Induces Neuropathic Pain by Interacting with NECAB2.

Applications

Unspecified application

Species

Unspecified reactive species

Yuetao Ma,Qilong Deng,Shanggeng Li,Meizhen Chen,Bingxin Jin,Mingcang Wang
View all publications
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Product promise

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