AB227908

Anti-Necdin antibody

Name: Anti-Necdin antibody (ab227908) | Abcam
Brand: Abcam Limited
SKU: AB227908
Price: 525 USD
Availability: InStock

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(21 Publications)

Rabbit Polyclonal Necdin antibody. Suitable for WB, IHC-Fr and reacts with Mouse samples. Cited in 21 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ndn aa 50 to C-terminus.

View Alternative Names

Necdin, Ndn

3 Images

Immunohistochemistry (Frozen sections) - Anti-Necdin antibody (AB227908)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Necdin antibody (AB227908)

Frozen sections of mouse E13.5 forebrain stained for Necdin using ab227908 at 1/500 dilution in immunohistochemical analysis. CX, Cortex; LV, lateral ventricle; LGE, lateral ganglionic eminence; SP, septum.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Necdin antibody (AB227908)

Cryosections of cervical dorsal root ganglion tissues from wild type (left panel) and Necdin-knockout (right panel) mouse E14.5 stained for Necdin using ab227908 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Necdin antibody (AB227908)

The larger size may reflect post-translational modifications such as ubiquitination and phosphorylation at several sites.

All lanes:

Western blot - Anti-Necdin antibody (ab227908) at 1/3000 dilution

All lanes:

Mouse E16.5 embryo cerebral cortex lysate at 20 µg

Predicted band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-Fr, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Ndn aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25233

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 6.5 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Necdin also known simply as NDN is a protein playing an important mechanical role in cellular functioning. It has a molecular mass of approximately 33 kDa. Necdin is expressed widely in postmitotic neurons in the brain and spinal cord where it contributes to the proper functioning of these cells. The protein also appears in other tissues but at notably lower levels. Necdin primarily functions as a transcriptional repressor that interacts with other molecules to regulate gene expression.

Biological function summary

Necdin carries out its function by forming complexes with other proteins such as p53 and E2F1 which regulate the cell cycle and apoptosis. Through interacting with these complexes Necdin modulates transcriptional activities that affect neuronal development and differentiation. Besides its role in neurons Necdin’s involvement in other cell types suggests it has a broader impact on cell proliferation and survival.

Pathways

Necdin fits significantly into the cell survival and apoptosis pathways. It interacts with p53 an important protein in the apoptosis pathway regulating cell death and preventing uncontrolled cell proliferation. Necdin also associates with the Retinoblastoma protein (Rb) pathway through E2F1 where it influences cell cycle progression. These interactions highlight its contribution to maintaining physiological balance and preventing aberrant cellular processes.

Necdin shows a connection to conditions like Prader-Willi Syndrome (PWS) and certain cancers. PWS caused by the absence of paternal Necdin expression results from deletions or disruptions in chromosome 15q11-q13 which impacts the development of the nervous and endocrine systems. Necdin's interaction with proteins like E2F1 highlights its involvement in cancer progression where dysregulation affects cell cycle control. Therefore Necdin's function and interaction with other proteins have significant implications for both developmental and pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Also functions as a transcription factor and directly binds to specific guanosine-rich DNA sequences.

See full target information Ndn

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:31605 PubMed39738217

2024

Prader-Willi syndrome protein necdin regulates the nucleocytoplasmic distribution and dopaminergic neuron development.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Yichun Zhang,Ying Hu,Xiangrong Tang,Zishan Gong,Ren-Bin Lu,Jia-da Li

Cell discovery 10:110 PubMed39438452

2024

A spatiotemporal transcriptomic atlas of mouse placentation.

Applications

Unspecified application

Species

Unspecified reactive species

Yanting Wu,Kaizhen Su,Ying Zhang,Langchao Liang,Fei Wang,Siyue Chen,Ling Gao,Qiutong Zheng,Cheng Li,Yunfei Su,Yiting Mao,Simeng Zhu,Chaochao Chai,Qing Lan,Man Zhai,Xin Jin,Jinglan Zhang,Xun Xu,Yu Zhang,Ya Gao,Hefeng Huang

iScience 27:109974 PubMed38832028

2024

Deficiency in Prader-Willi syndrome gene leads to attenuated cardiac contractility.

Applications

Unspecified application

Species

Unspecified reactive species

Yufan Dong,Renbin Lu,Hui Cao,Jing Zhang,Xiushan Wu,Yun Deng,Jia-Da Li

Nature communications 7:10943 PubMed26971449

2016

Promotion of mitochondrial biogenesis by necdin protects neurons against mitochondrial insults.

Applications

WB, IHC-Fr

Species

Mouse, Mouse

Koichi Hasegawa,Toru Yasuda,Chinatsu Shiraishi,Kazushiro Fujiwara,Serge Przedborski,Hideki Mochizuki,Kazuaki Yoshikawa

Cellular signalling 28:94-107 PubMed26655377

2015

Necdin controls EGFR signaling linked to astrocyte differentiation in primary cortical progenitor cells.

Applications

WB, IP

Species

Mouse, Mouse

Izumi Fujimoto,Koichi Hasegawa,Kazushiro Fujiwara,Masashi Yamada,Kazuaki Yoshikawa

PloS one 9:e84460 PubMed24392139

2014

Antagonistic interplay between necdin and Bmi1 controls proliferation of neural precursor cells in the embryonic mouse neocortex.

Applications

IP, WB, IF

Species

Mouse, Mouse, Mouse

Ryohei Minamide,Kazushiro Fujiwara,Koichi Hasegawa,Kazuaki Yoshikawa

Molecular and cellular biology 32:2722-37 PubMed22586272

2012

Nerve growth factor-induced cell cycle reentry in newborn neurons is triggered by p38MAPK-dependent E2F4 phosphorylation.

Applications

IP, WB

Species

Chicken, Chicken

Sandra M Morillo,Erika P Abanto,María J Román,José M Frade

Blood 114:4383-92 PubMed19770359

2009

Necdin restricts proliferation of hematopoietic stem cells during hematopoietic regeneration.

Applications

Species

Mouse

Yasushi Kubota,Masatake Osawa,Lars Martin Jakt,Kazuaki Yoshikawa,Shin-Ichi Nishikawa

Physiological genomics 30:156-71 PubMed17374844

2007

Single mage gene in the chicken genome encodes CMage, a protein with functional similarities to mammalian type II Mage proteins.

Applications

ICC/IF, IHC-Fr

Species

Mouse, Mouse

Noelia López-Sánchez,Zaira González-Fernández,Michio Niinobe,Kazuaki Yoshikawa,José María Frade

BMC developmental biology 6:56 PubMed17116257

2006

Sensory defects in Necdin deficient mice result from a loss of sensory neurons correlated within an increase of developmental programmed cell death.

Applications

Species

Mouse

David Andrieu,Hamid Meziane,Fabienne Marly,Corinne Angelats,Pierre-Alain Fernandez,Françoise Muscatelli

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Necdin antibody

Immunohistochemistry (Frozen sections) - Anti-Necdin antibody (AB227908)

Immunohistochemistry (Frozen sections) - Anti-Necdin antibody (AB227908)

Western blot - Anti-Necdin antibody (AB227908)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

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