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AB233085

Anti-Nectin 2 antibody

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Rabbit Polyclonal Nectin 2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NECTIN2 aa 50-400.

View Alternative Names

CD112, HVEB, PRR2, PVRL2, NECTIN2, Nectin-2, Herpes virus entry mediator B, Nectin cell adhesion molecule 2, Poliovirus receptor-related protein 2, Herpesvirus entry mediator B, HveB

5 Images
Western blot - Anti-Nectin 2 antibody (AB233085)
  • WB

Supplier Data

Western blot - Anti-Nectin 2 antibody (AB233085)

All lanes:

Western blot - Anti-Nectin 2 antibody (ab233085) at 2 µg/mL

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 58 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody (AB233085)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody (AB233085)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Nectin 2 using ab233085 in at 20 μg/ml immunohistochemical analysis. DAB staining.

Western blot - Anti-Nectin 2 antibody (AB233085)
  • WB

Supplier Data

Western blot - Anti-Nectin 2 antibody (AB233085)

All lanes:

Western blot - Anti-Nectin 2 antibody (ab233085) at 2 µg/mL

All lanes:

Mouse testis tissue lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 58 kDa

false

Western blot - Anti-Nectin 2 antibody (AB233085)
  • WB

Unknown

Western blot - Anti-Nectin 2 antibody (AB233085)

All lanes:

Western blot - Anti-Nectin 2 antibody (ab233085) at 2 µg/mL

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 58 kDa

false

Western blot - Anti-Nectin 2 antibody (AB233085)
  • WB

Supplier Data

Western blot - Anti-Nectin 2 antibody (AB233085)

All lanes:

Western blot - Anti-Nectin 2 antibody (ab233085) at 2 µg/mL

All lanes:

Recombinant human Nectin 2 protein

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NECTIN2 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

Q92692

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nectin 2 also known as CD112 is a transmembrane cell adhesion molecule belonging to the immunoglobulin superfamily. It plays a critical role in mediating cell-cell adhesion by forming homophilic and heterophilic interactions with other nectins. The protein has a mass of approximately 65 kDa and shows high expression in epithelial and endothelial cells. Nectin 2 has been identified in various tissues including the skin lung and kidney reflecting its importance in maintaining tissue integrity.
Biological function summary

Nectin 2 facilitates cell adhesion and communication playing an important role in the formation of adherens junctions. It serves as part of a larger biological complex with proteins such as afadin linking it to the actin cytoskeleton. Through these interactions Nectin 2 influences the signaling pathways that regulate tissue development and maintenance. Its ability to bind herpes virus entry mediator A (HVEM) further demonstrates its function in viral entry highlighting its diverse biological roles.

Pathways

Nectin 2 participates in the cell adhesion and immune response pathways. It works closely with Nectin 1 and Cadherins within these pathways to mediate cell-cell junction formation and signaling. The interaction of Nectin 2 with intracellular proteins like afadin facilitates its role in the immunological synapse a critical zone for immune cell signaling. These pathways highlight its significance in both maintaining cellular architecture and modulating immune responses.

Nectin 2 relates to cancer and viral infections. Its altered expression or dysfunction has links with tumor progression and metastasis particularly in epithelial-origin cancers like colorectal cancer. Through its role as a herpesvirus entry receptor it also connects to herpes simplex virus (HSV) infections. The interactions between Nectin 2 and other nectins such as Nectin 3 may affect cell proliferation and viral entry expanding the understanding of its involvement in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive (PubMed : 26755705). Probable cell adhesion protein (PubMed : 9657005).. (Microbial infection) Acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).
See full target information NECTIN2
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