AB47171

Anti-Nectin 2 antibody [B-C12] - BSA and Azide free

Name: Anti-Nectin 2 antibody [B-C12] - BSA and Azide free (ab47171) | Abcam
Brand: Abcam Limited
SKU: AB47171

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(2 Publications)

Mouse Monoclonal Nectin 2 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing NECTIN2 protein.

View Alternative Names

CD112, HVEB, PRR2, PVRL2, NECTIN2, Nectin-2, Herpes virus entry mediator B, Nectin cell adhesion molecule 2, Poliovirus receptor-related protein 2, Herpesvirus entry mediator B, HveB

1 Images

Flow Cytometry - Anti-Nectin 2 antibody [B-C12] - BSA and Azide free (AB47171)

Flow Cyt

Unknown

Flow Cytometry - Anti-Nectin 2 antibody [B-C12] - BSA and Azide free (AB47171)

A typical staining pattern with ab47171 monoclonal antibody of KG-1 cell line.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

B-C12

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

Cell preparation containing NECTIN2 protein. The exact immunogen used to generate this antibody is proprietary information.

Q92692

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 4^th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 - 7.5 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nectin 2 also known as CD112 is a transmembrane cell adhesion molecule belonging to the immunoglobulin superfamily. It plays a critical role in mediating cell-cell adhesion by forming homophilic and heterophilic interactions with other nectins. The protein has a mass of approximately 65 kDa and shows high expression in epithelial and endothelial cells. Nectin 2 has been identified in various tissues including the skin lung and kidney reflecting its importance in maintaining tissue integrity.

Biological function summary

Nectin 2 facilitates cell adhesion and communication playing an important role in the formation of adherens junctions. It serves as part of a larger biological complex with proteins such as afadin linking it to the actin cytoskeleton. Through these interactions Nectin 2 influences the signaling pathways that regulate tissue development and maintenance. Its ability to bind herpes virus entry mediator A (HVEM) further demonstrates its function in viral entry highlighting its diverse biological roles.

Pathways

Nectin 2 participates in the cell adhesion and immune response pathways. It works closely with Nectin 1 and Cadherins within these pathways to mediate cell-cell junction formation and signaling. The interaction of Nectin 2 with intracellular proteins like afadin facilitates its role in the immunological synapse a critical zone for immune cell signaling. These pathways highlight its significance in both maintaining cellular architecture and modulating immune responses.

Nectin 2 relates to cancer and viral infections. Its altered expression or dysfunction has links with tumor progression and metastasis particularly in epithelial-origin cancers like colorectal cancer. Through its role as a herpesvirus entry receptor it also connects to herpes simplex virus (HSV) infections. The interactions between Nectin 2 and other nectins such as Nectin 3 may affect cell proliferation and viral entry expanding the understanding of its involvement in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive (PubMed : 26755705). Probable cell adhesion protein (PubMed : 9657005).. (Microbial infection) Acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).

See full target information NECTIN2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 6:284 PubMed26106390

2015

NKG2D is a Key Receptor for Recognition of Bladder Cancer Cells by IL-2-Activated NK Cells and BCG Promotes NK Cell Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Eva María García-Cuesta,Sheila López-Cobo,Mario Álvarez-Maestro,Gloria Esteso,Gema Romera-Cárdenas,Mercedes Rey,Robin L Cassady-Cain,Ana Linares,Alejandro Valés-Gómez,Hugh Thomson Reyburn,Luis Martínez-Piñeiro,Mar Valés-Gómez

PloS one 8:e66825 PubMed23762498

2013

Myxoma virus infection promotes NK lysis of malignant gliomas in vitro and in vivo.

Applications

Flow Cyt

Species

Human

Henry Ogbomo,Franz J Zemp,Xueqing Lun,Jiqing Zhang,Danuta Stack,Masmudur M Rahman,Grant McFadden,Christopher H Mody,Peter A Forsyth

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nectin 2 antibody [B-C12] - BSA and Azide free

Flow Cytometry - Anti-Nectin 2 antibody [B-C12] - BSA and Azide free (AB47171)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise