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Rabbit Recombinant Monoclonal Nectin 2 antibody. Suitable for IHC-P, WB, IP, Flow Cyt and reacts with Mouse samples.

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Images

Flow Cytometry - Anti-Nectin 2 antibody [EPR28277-22] (AB314229), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (AB314229), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (AB314229), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (AB314229), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (AB314229), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIPFlow CytICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein.

    Alternative names

    Nectin-2, Herpes virus entry mediator B, Murine herpes virus entry protein B, Nectin cell adhesion molecule 2, Poliovirus receptor homolog, Poliovirus receptor-related protein 2, Herpesvirus entry mediator B, HveB, mHveB, Nectin2, Mph, Pvr, Pvs, Pvrl2

    Recommended products

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    Rabbit Recombinant Monoclonal Nectin 2 antibody. Suitable for IHC-P, WB, IP, Flow Cyt and reacts with Mouse samples.

    Alternative names

    Nectin-2, Herpes virus entry mediator B, Murine herpes virus entry protein B, Nectin cell adhesion molecule 2, Poliovirus receptor homolog, Poliovirus receptor-related protein 2, Herpesvirus entry mediator B, HveB, mHveB, Nectin2, Mph, Pvr, Pvs, Pvrl2

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    EPR28277-22

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Supplementary info

    Activity summary

    Nectin 2 also known as CD112 is a transmembrane cell adhesion molecule belonging to the immunoglobulin superfamily. It plays a critical role in mediating cell-cell adhesion by forming homophilic and heterophilic interactions with other nectins. The protein has a mass of approximately 65 kDa and shows high expression in epithelial and endothelial cells. Nectin 2 has been identified in various tissues including the skin lung and kidney reflecting its importance in maintaining tissue integrity.

    Biological function summary

    Nectin 2 facilitates cell adhesion and communication playing an important role in the formation of adherens junctions. It serves as part of a larger biological complex with proteins such as afadin linking it to the actin cytoskeleton. Through these interactions Nectin 2 influences the signaling pathways that regulate tissue development and maintenance. Its ability to bind herpes virus entry mediator A (HVEM) further demonstrates its function in viral entry highlighting its diverse biological roles.

    Pathways

    Nectin 2 participates in the cell adhesion and immune response pathways. It works closely with Nectin 1 and Cadherins within these pathways to mediate cell-cell junction formation and signaling. The interaction of Nectin 2 with intracellular proteins like afadin facilitates its role in the immunological synapse a critical zone for immune cell signaling. These pathways highlight its significance in both maintaining cellular architecture and modulating immune responses.

    Associated diseases and disorders

    Nectin 2 relates to cancer and viral infections. Its altered expression or dysfunction has links with tumor progression and metastasis particularly in epithelial-origin cancers like colorectal cancer. Through its role as a herpesvirus entry receptor it also connects to herpes simplex virus (HSV) infections. The interactions between Nectin 2 and other nectins such as Nectin 3 may affect cell proliferation and viral entry expanding the understanding of its involvement in disease mechanisms.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    8 product images

    • Flow Cytometry - Anti-Nectin 2 antibody [EPR28277-22] (ab314229), expandable thumbnail

      Flow Cytometry - Anti-Nectin 2 antibody [EPR28277-22] (ab314229)

      Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / NIH/3T3 (mouse embryonic fibroblast, Right) cells labelling Nectin 2 with ab314229 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Low expression: EL4. Gated on viable cells.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (ab314229), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (ab314229)

      Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Nectin 2 with ab314229 at 1/500 (1.012 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Low expression: almost no staining on mouse skeletal muscle (PMID: 10744716). The section was incubated with ab314229 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (ab314229), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (ab314229)

      Immunohistochemical analysis of paraffin-embedded mouse breast tissue labeling Nectin 2 with ab314229 at 1/500 (1.012 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Low expression: weak staining on mouse breast. The section was incubated with ab314229 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (ab314229), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (ab314229)

      Immunohistochemical analysis of paraffin-embedded mouse pancreatic tumor tissue labeling Nectin 2 with ab314229 at 1/500 (1.012 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse pancreatic tumor. The section was incubated with ab314229 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (ab314229), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (ab314229)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Nectin 2 with ab314229 at 1/500 (1.012 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse testis. The section was incubated with ab314229 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (ab314229), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 2 antibody [EPR28277-22] (ab314229)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Nectin 2 with ab314229 at 1/500 (1.012 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse liver. The section was incubated with ab314229 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunoprecipitation - Anti-Nectin 2 antibody [EPR28277-22] (ab314229), expandable thumbnail

      Immunoprecipitation - Anti-Nectin 2 antibody [EPR28277-22] (ab314229)

      Nectin 2 was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab314229 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314229 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
      Lane 1: Mouse testis tissue lysate
      Lane 2: ab314229 IP in Mouse testis tissue lysate
      Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314229 in mouse testis tissue lysate
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      All lanes: Immunoprecipitation - Anti-Nectin 2 antibody [EPR28277-22] (ab314229) at 1/30 dilution

      All lanes: Mouse testis tissue lysate

      Secondary

      All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

      Exposure time: 180s

    • Western blot - Anti-Nectin 2 antibody [EPR28277-22] (ab314229), expandable thumbnail

      Western blot - Anti-Nectin 2 antibody [EPR28277-22] (ab314229)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Low expression: ovary, breast(PMID: 23758976)
      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      All lanes: Western blot - Anti-Nectin 2 antibody [EPR28277-22] (ab314229) at 1/1000 dilution

      Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

      Lane 2: Mouse testis tissue lysate at 20 µg

      Lane 3: Mouse ovary tissue lysate at 20 µg

      Lane 4: Mouse breast tissue lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 70 kDa, 80 kDa

      Exposure time: 180s

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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