Rabbit Recombinant Monoclonal Nectin 3 antibody. Carrier free. Suitable for IHC-P, WB, IP and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (AB314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420285.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (AB314220)")
![Immunoprecipitation - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (AB314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--immunoprecipitation-img420271.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (AB314220)")
![Western blot - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (AB314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--western-blot-img420261.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (AB314220)")
![Western blot - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (AB314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--western-blot-img420260.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (AB314220)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (AB314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420288.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (AB314220)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | IP | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Cell adhesion molecule that promotes cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions (PubMed:16216929). Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1 (PubMed:16216929). Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation (PubMed:16216929). Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells (By similarity). Also involved in the formation of cell-cell junctions, including adherens junctions and synapses (By similarity). Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN1: NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types (By similarity). Plays a role in the morphology of the ciliary body (By similarity).
Alternative names
CD113, PRR3, PVRL3, NECTIN3, Nectin-3, CDw113, Nectin cell adhesion molecule 3, Poliovirus receptor-related protein 3
Recommended products
Rabbit Recombinant Monoclonal Nectin 3 antibody. Carrier free. Suitable for IHC-P, WB, IP and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26326-22
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314220 is the carrier-free version of Anti-Nectin 3 antibody [EPR26326-22] ab314219.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nectin-3 also known as CD113 or PVRL3 is an immunoglobulin-like cell adhesion molecule. It plays an important role in cell-cell adhesion by forming homo- and heterophilic trans-dimers with other nectins and nectin-like molecules. Nectin-3 has a molecular mass of approximately 83 kDa. Its expression is generally observed in neural tissues such as the brain as well as in testis and various epithelial tissues. This distribution allows it to contribute to diverse cellular interactions critical for tissue organization and development.
- Biological function summary
Nectin-3 participates in the formation and maintenance of adherens junctions by interacting with cadherins and actin filaments. It is a member of the Nectin family which includes Nectin-1 Nectin-2 and Nectin-4 known for their roles in cell adhesion. The function of Nectin-3 is integral to the establishment of tight junctions contributing to the structural integrity and function of epithelial and endothelial cells. It often works as part of a complex with afadin which supports its connection to the actin cytoskeleton.
- Pathways
Nectin-3 acts in the formation of synapses and is involved in signaling pathways related to cell adhesion such as the actin cytoskeleton regulation pathway. It plays a significant role in the activation of the Ras and Rac small GTPase signaling pathways facilitating changes in cytoskeletal dynamics during cell movement and morphogenesis. Nectin-3 closely associates with other proteins in the Nectin family such as Nectin-1 and Nectin-2 in these pathways enhancing intercellular communication.
- Associated diseases and disorders
Mutations or dysregulation of Nectin-3 have been linked to conditions like mental retardation and developmental abnormalities. It is particularly relevant in the context of intellectual disability where alterations in cell adhesion and signaling can impair proper neuronal connectivity and brain function. Furthermore Nectin-3 interacts with PVRL proteins highlighting its possible involvement in cancer progression and metastasis through altered cell adhesion properties.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420285.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)
This data was developed using Anti-Nectin 3 antibody [EPR26326-22] ab314219, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Nectin 3 with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Positive staining on human testis. The section was incubated with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)![Immunoprecipitation - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--immunoprecipitation-img420271.jpg "Immunoprecipitation - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)")
Immunoprecipitation - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)
This data was developed using Anti-Nectin 3 antibody [EPR26326-22] ab314219, the same antibody clone in a different buffer formulation.
Nectin 3 was immunoprecipitated from 0.35 mg HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lane 2: Anti-Nectin 3 antibody [EPR26326-22] ab314219 IP in HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Nectin 3 antibody [EPR26326-22] ab314219 in HCT116 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 137 seconds
The identity of the lower MW band at approximately 25 kDa (in lane 2) is unknown.All lanes: Immunoprecipitation - Anti-Nectin 3 antibody [EPR26326-22] (Anti-Nectin 3 antibody [EPR26326-22] ab314219) at 1/30 dilution
All lanes: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 137s
![Western blot - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--western-blot-img420261.jpg "Western blot - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)")
Western blot - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)
This data was developed using Anti-Nectin 3 antibody [EPR26326-22] ab314219, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: U-937 (PMID: 11024295).
Nectin 3 is a glycoprotein of approximately 100 kDa and detected as a 60 kDa band after treated with Protein Deglycosylation MIX II.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-2: 10 seconds, Lane 3-5: 5 seconds.All lanes: Western blot - Anti-Nectin 3 antibody [EPR26326-22] (Anti-Nectin 3 antibody [EPR26326-22] ab314219) at 1/1000 dilution
Lane 1: Untreated HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HCT 116 whole cell lysate treated with Protein Deglycosylation MIX II at 15 µg
Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg
Lane 5: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 60-100 kDa
![Western blot - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--western-blot-img420260.jpg "Western blot - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)")
Western blot - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)
This data was developed using Anti-Nectin 3 antibody [EPR26326-22] ab314219, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: heart (PMID: 11024295), colon (PMID: 11024295).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: 10 secondsAll lanes: Western blot - Anti-Nectin 3 antibody [EPR26326-22] (Anti-Nectin 3 antibody [EPR26326-22] ab314219) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
Lane 3: Human colon tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 60-100 kDa
Exposure time: 10s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420288.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)
This data was developed using Anti-Nectin 3 antibody [EPR26326-22] ab314219, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Nectin 3 with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Negative control: no staining on human cardiac muscle. The section was incubated with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420287.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)
This data was developed using Anti-Nectin 3 antibody [EPR26326-22] ab314219, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Nectin 3 with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Positive staining on human lung carcinoma. The section was incubated with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420286.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)
This data was developed using Anti-Nectin 3 antibody [EPR26326-22] ab314219, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Nectin 3 with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Positive staining on human placenta. The section was incubated with Anti-Nectin 3 antibody [EPR26326-22] ab314219 at 4°C overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420287.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220), expandable thumbnail](https://content.abcam.com/products/images/nectin-3-antibody-epr26326-22-bsa-and-azide-free-ab314220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420286.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin 3 antibody [EPR26326-22] - BSA and Azide free (ab314220)")
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