AB155692

Anti-Nectin-4 antibody

Name: Anti-Nectin-4 antibody. Rabbit polyclonal (ab155692) | Abcam
Brand: Abcam Limited
SKU: AB155692
Price: 435 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal Nectin-4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NECTIN4 aa 1-300.

View Alternative Names

LNIR, PRR4, PVRL4, NECTIN4, Nectin-4, Ig superfamily receptor LNIR, Nectin cell adhesion molecule 4, Poliovirus receptor-related protein 4

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin-4 antibody (AB155692)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin-4 antibody (AB155692)

Immunohistochemical analysis of paraffin embedded MCF7 xenograft labeling Nectin-4 with ab155692 antibody at 1/500.

Unknown

Western blot - Anti-Nectin-4 antibody (AB155692)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Nectin-4 antibody (ab155692) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NECTIN4 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q96NY8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nectin-4 also known as PVRL4 is a member of the immunoglobulin-like adhesion molecule family. It has a molecular weight of approximately 66 kDa. Nectin-4 plays an important role in cell-cell adhesion by forming trans-dimers promoting cellular cohesion. It is expressed in tissues such as placenta embryonic tissues and several epithelial cancers indicating its potential involvement in developmental processes and pathological conditions. When utilizing methods such as 'nectin-4 ELISA' and 'nectin-4 IHC' researchers can study its expression extensively across different tissue samples.

Biological function summary

Nectin-4 functions as an important adhesion molecule that contributes to the maintenance and organization of cell junctions. It is often part of larger adhesive complexes that include afadin which links nectin proteins to the actin cytoskeleton stabilizing cell structure. Besides its role in maintaining cell-cell adhesion nectin-4 is significant in processes like cellular polarization and migration. These properties make it an important target for understanding tissue development and integrity as well as cancer metastasis.

Pathways

Nectin-4 belongs to the pathways regulating cell adhesion and the cytoskeletal organization. It is closely associated with the cadherin and integrin signaling pathways where it collaborates with other nectin family members and afadin to regulate these networks. These interactions impact cellular processes such as signal transduction and intercellular communication critical for tissue maintenance. Studies involving 'nectin expression' help further elucidate these pathway connections and their implications in healthy and diseased tissues.

Nectin-4 shows significant correlation with certain cancers such as breast and lung carcinoma. Its elevated expression in tumor tissues suggests a role in tumorigenesis and cancer progression. The protein's interaction with oncogenes like HER2 indicates a possible pathway by which nectin-4 affects cancer cell proliferation and survival. Understanding 'nectin-4 expression' in the context of these diseases aids in identifying potential biomarkers and therapeutic targets for cancer diagnosis and treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.. (Microbial infection) Acts as a receptor for measles virus.

See full target information NECTIN4

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:785-796 PubMed39582282

2024

Assessing the expression of Nectin-4 in solid tumors by immunohistochemistry - what do we know?

Applications

Unspecified application

Species

Unspecified reactive species

Christine Sanders,Glen Kristiansen

Chinese medical journal 137:338-349 PubMed38105538

2023

Estrogen receptor beta suppresses the androgen receptor oncogenic effects in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Xu,Kun Xu,Lingling Fan,Xintong Li,Yiqiu Liu,Fang Yang,Chengjun Zhu,Xiaoxiang Guan

International journal of molecular sciences 24: PubMed37958809

2023

mRNA and Protein Expression in Human Fetal Membrane Cells: Potential Biomarkers for Preterm Prelabor Rupture of the Fetal Membranes?

Applications

Unspecified application

Species

Unspecified reactive species

Emmeli Mikkelsen,Berthold Huppertz,Ripudaman Singh,Katarina Ravn,Lotte Hatt,Mogens Kruhøffer,Rheanna Urrabaz-Garza,Niels Uldbjerg,Ramkumar Menon,Torben Steiniche

OncoTargets and therapy 15:1519-1529 PubMed36545447

2022

Targeted Treatment of Locally Advanced and Metastatic Urothelial Cancer: Enfortumab Vedotin in Context.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Hoffman-Censits,Laneisha Maldonado

Medical science monitor : international medical journal of experimental and clinical research 25:10089-10094 PubMed31883369

2019

The Prognostic Role of Expression of Nectin-4 in Esophageal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Lin,Huijun Hu,Yongquan Pan,Shuangquan Gao

Autophagy 16:842-861 PubMed31318632

2019

Autophagy induction by the pathogen receptor NECTIN4 and sustained autophagy contribute to peste des petits ruminants virus infectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yang,Qinghong Xue,Jiaona Guo,Xueping Wang,Yanming Zhang,Kangkang Guo,Wei Li,Shuying Chen,Tianxia Xue,Xuefeng Qi,Jingyu Wang

Journal of neuropathology and experimental neurology 72:681-96 PubMed23771216

2013

Wild-type measles virus infection upregulates poliovirus receptor-related 4 and causes apoptosis in brain endothelial cells by induction of tumor necrosis factor-related apoptosis-inducing ligand.

Applications

Unspecified application

Species

Unspecified reactive species

Hani'ah Abdullah,Brenda Brankin,Clare Brady,Sara Louise Cosby

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nectin-4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nectin-4 antibody (AB155692)

Western blot - Anti-Nectin-4 antibody (AB155692)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise