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AB235897

Anti-Nectin-4 antibody

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(4 Publications)

Rabbit Polyclonal Nectin-4 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NECTIN4 aa 50-200.

View Alternative Names

LNIR, PRR4, PVRL4, NECTIN4, Nectin-4, Ig superfamily receptor LNIR, Nectin cell adhesion molecule 4, Poliovirus receptor-related protein 4

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nectin-4 antibody (AB235897)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nectin-4 antibody (AB235897)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells stained for Nectin-4 using ab235897 at 1/133 dilution in ICC/IF. The cells were blocked in 10% normal goat serum and incubated with primary antibody at 4°C overnight. Secondary antibody was Aleza Fluor® 488-conjugated goat anti-rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NECTIN4 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q96NY8

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Alternative Products

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB40772

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

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Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB40772)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nectin-4 also known as PVRL4 is a member of the immunoglobulin-like adhesion molecule family. It has a molecular weight of approximately 66 kDa. Nectin-4 plays an important role in cell-cell adhesion by forming trans-dimers promoting cellular cohesion. It is expressed in tissues such as placenta embryonic tissues and several epithelial cancers indicating its potential involvement in developmental processes and pathological conditions. When utilizing methods such as 'nectin-4 ELISA' and 'nectin-4 IHC' researchers can study its expression extensively across different tissue samples.
Biological function summary

Nectin-4 functions as an important adhesion molecule that contributes to the maintenance and organization of cell junctions. It is often part of larger adhesive complexes that include afadin which links nectin proteins to the actin cytoskeleton stabilizing cell structure. Besides its role in maintaining cell-cell adhesion nectin-4 is significant in processes like cellular polarization and migration. These properties make it an important target for understanding tissue development and integrity as well as cancer metastasis.

Pathways

Nectin-4 belongs to the pathways regulating cell adhesion and the cytoskeletal organization. It is closely associated with the cadherin and integrin signaling pathways where it collaborates with other nectin family members and afadin to regulate these networks. These interactions impact cellular processes such as signal transduction and intercellular communication critical for tissue maintenance. Studies involving 'nectin expression' help further elucidate these pathway connections and their implications in healthy and diseased tissues.

Nectin-4 shows significant correlation with certain cancers such as breast and lung carcinoma. Its elevated expression in tumor tissues suggests a role in tumorigenesis and cancer progression. The protein's interaction with oncogenes like HER2 indicates a possible pathway by which nectin-4 affects cancer cell proliferation and survival. Understanding 'nectin-4 expression' in the context of these diseases aids in identifying potential biomarkers and therapeutic targets for cancer diagnosis and treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.. (Microbial infection) Acts as a receptor for measles virus.
See full target information NECTIN4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 29:107 PubMed39736923

2024

Primary tracheal hyalinizing clear cell carcinoma with EWSR1 rearrangement: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Wenkang Zong,Guozheng Gao,Xike Lu,Daqiang Sun

Cell death & disease 14:107 PubMed36774351

2023

CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuehong Shen,Yuling Chen,Yuntao Lin,Yicun Li,Pengfei Liu,Biru Zhang,Yufan Wang,King-Chi Chan,Nai-Ki Mak,Michael Kahn,Robert Z Qi,Hongyu Yang

Scientific reports 12:4031 PubMed35256687

2022

Nectin cell adhesion molecule 4 regulates angiogenesis through Src signaling and serves as a novel therapeutic target in angiosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Tanaka,Maho Murata,Keiko Tanegashima,Yoshinao Oda,Takamichi Ito

Biomedicines 9: PubMed33808400

2021

Nectin Cell Adhesion Molecule 4 (NECTIN4) Expression in Cutaneous Squamous Cell Carcinoma: A New Therapeutic Target?

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Tanaka,Maho Murata,Yoshinao Oda,Masutaka Furue,Takamichi Ito
View all publications
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