Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269)

This data was developed using ab317268, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling Nectin-4 with ab317268 at 1/50 (10.14 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing membrane staining in T-47D cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Low expression : A549 (PMID : 21901103).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269)

This data was developed using ab317268, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of DLD-1 (human colorectal adenocarcinoma epithelial cell) (Right) / HeLa (human cervical adenocarcinoma epithelial cell) (Left) cells labelling Nectin-4 with ab317268 at 1/500 dilution (0.1ug)/red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : HeLa (PMID : 21901103).

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269)

This data was developed using ab317268, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of T-47D (human ductal breast epithelial tumor epithelial cel) (Right) / A549 (human lung carcinoma epithelial cell) (Left) cells labelling Nectin-4 with ab317268 at 1/500 dilution (0.1ug)/red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Low expression : A549 (PMID : 21901103).

• Dot

Supplier Data

Dot Blot - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269)

This data was developed using ab317268, the same antibody clone in a different buffer formulation.

Dot blot analysis of Nectin-4 using ab317268 at 1 : 1000 (0.507 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-Nectin-4 antibody [EPR29074-48] (<a href='/en-us/products/primary-antibodies/nectin-4-antibody-epr29074-48-ab317268'>ab317268</a>) at 1/1000 dilution

All lanes:

Human Nectin-4 fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s