Rabbit Recombinant Monoclonal Nectin-4 antibody. Carrier free. Suitable for Dot, Flow Cyt, ICC/IF and reacts with Recombinant fragment - Human, Human samples.
Images
![Dot Blot - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269), expandable thumbnail](https://content.abcam.com/products/images/nectin-4-antibody-epr29074-48-bsa-and-azide-free-ab317269--dot-blot-img429632.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269)")
![Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269), expandable thumbnail](https://content.abcam.com/products/images/nectin-4-antibody-epr29074-48-bsa-and-azide-free-ab317269--flow-cytometry-img429710.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269)")
![Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269), expandable thumbnail](https://content.abcam.com/products/images/nectin-4-antibody-epr29074-48-bsa-and-azide-free-ab317269--flow-cytometry-img429709.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269)")
![Immunocytochemistry/ Immunofluorescence - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269), expandable thumbnail](https://content.abcam.com/products/images/nectin-4-antibody-epr29074-48-bsa-and-azide-free-ab317269--immunocytochemistry-immunofluorescence-img429691.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (AB317269)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | Flow Cyt | ICC/IF | WB | IP | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment - Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells. (Microbial infection) Acts as a receptor for measles virus.
Alternative names
LNIR, PRR4, PVRL4, NECTIN4, Nectin-4, Ig superfamily receptor LNIR, Nectin cell adhesion molecule 4, Poliovirus receptor-related protein 4
Recommended products
Rabbit Recombinant Monoclonal Nectin-4 antibody. Carrier free. Suitable for Dot, Flow Cyt, ICC/IF and reacts with Recombinant fragment - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29074-48
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317269 is the carrirer-free version of Anti-Nectin-4 antibody [EPR29074-48] ab317268.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nectin-4 also known as PVRL4 is a member of the immunoglobulin-like adhesion molecule family. It has a molecular weight of approximately 66 kDa. Nectin-4 plays an important role in cell-cell adhesion by forming trans-dimers promoting cellular cohesion. It is expressed in tissues such as placenta embryonic tissues and several epithelial cancers indicating its potential involvement in developmental processes and pathological conditions. When utilizing methods such as 'nectin-4 ELISA' and 'nectin-4 IHC' researchers can study its expression extensively across different tissue samples.
- Biological function summary
Nectin-4 functions as an important adhesion molecule that contributes to the maintenance and organization of cell junctions. It is often part of larger adhesive complexes that include afadin which links nectin proteins to the actin cytoskeleton stabilizing cell structure. Besides its role in maintaining cell-cell adhesion nectin-4 is significant in processes like cellular polarization and migration. These properties make it an important target for understanding tissue development and integrity as well as cancer metastasis.
- Pathways
Nectin-4 belongs to the pathways regulating cell adhesion and the cytoskeletal organization. It is closely associated with the cadherin and integrin signaling pathways where it collaborates with other nectin family members and afadin to regulate these networks. These interactions impact cellular processes such as signal transduction and intercellular communication critical for tissue maintenance. Studies involving 'nectin expression' help further elucidate these pathway connections and their implications in healthy and diseased tissues.
- Associated diseases and disorders
Nectin-4 shows significant correlation with certain cancers such as breast and lung carcinoma. Its elevated expression in tumor tissues suggests a role in tumorigenesis and cancer progression. The protein's interaction with oncogenes like HER2 indicates a possible pathway by which nectin-4 affects cancer cell proliferation and survival. Understanding 'nectin-4 expression' in the context of these diseases aids in identifying potential biomarkers and therapeutic targets for cancer diagnosis and treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Dot Blot - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269), expandable thumbnail](https://content.abcam.com/products/images/nectin-4-antibody-epr29074-48-bsa-and-azide-free-ab317269--dot-blot-img429632.jpg "Dot Blot - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269)")
Dot Blot - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269)
Nectin-4 Dot Blot staining of Human Nectin-4 fragment using rabbit Anti-Nectin-4 antibody
This data was developed using Anti-Nectin-4 antibody [EPR29074-48] ab317268, the same antibody clone in a different buffer formulation.
Dot blot analysis of Nectin-4 using Anti-Nectin-4 antibody [EPR29074-48] ab317268 at 1:1000 (0.507 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
All lanes: Dot Blot - Anti-Nectin-4 antibody [EPR29074-48] (Anti-Nectin-4 antibody [EPR29074-48] ab317268) at 1/1000 dilution
All lanes: Human Nectin-4 fragment
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269), expandable thumbnail](https://content.abcam.com/products/images/nectin-4-antibody-epr29074-48-bsa-and-azide-free-ab317269--flow-cytometry-img429710.jpg "Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269)")
Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269)
This data was developed using Anti-Nectin-4 antibody [EPR29074-48] ab317268, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of DLD-1 (human colorectal adenocarcinoma epithelial cell) (Right) / HeLa (human cervical adenocarcinoma epithelial cell) (Left) cells labelling Nectin-4 with Anti-Nectin-4 antibody [EPR29074-48] ab317268 at 1/500 dilution (0.1ug)/red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression: HeLa (PMID:21901103).
![Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269), expandable thumbnail](https://content.abcam.com/products/images/nectin-4-antibody-epr29074-48-bsa-and-azide-free-ab317269--flow-cytometry-img429709.jpg "Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269)")
Flow Cytometry - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269)
This data was developed using Anti-Nectin-4 antibody [EPR29074-48] ab317268, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of T-47D (human ductal breast epithelial tumor epithelial cel) (Right) / A549 (human lung carcinoma epithelial cell) (Left) cells labelling Nectin-4 with Anti-Nectin-4 antibody [EPR29074-48] ab317268 at 1/500 dilution (0.1ug)/red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Low expression: A549 (PMID:21901103).
![Immunocytochemistry/ Immunofluorescence - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269), expandable thumbnail](https://content.abcam.com/products/images/nectin-4-antibody-epr29074-48-bsa-and-azide-free-ab317269--immunocytochemistry-immunofluorescence-img429691.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269)")
Immunocytochemistry/ Immunofluorescence - Anti-Nectin-4 antibody [EPR29074-48] - BSA and Azide free (ab317269)
This data was developed using Anti-Nectin-4 antibody [EPR29074-48] ab317268, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T-47D (human ductal breast epithelial tumor epithelial cell) cells labelling Nectin-4 with Anti-Nectin-4 antibody [EPR29074-48] ab317268 at 1/50 (10.14 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing membrane staining in T-47D cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Low expression: A549 (PMID:21901103).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
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