Rabbit Recombinant Monoclonal HEF1/NEDD-9 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NEDD9 aa 150-200.
Images
![Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111), expandable thumbnail](https://content.abcam.com/products/images/nedd-9-casl-hef1-antibody-blr248l-bsa-free-ab314111--western-blot-img420707.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111)")
![Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111), expandable thumbnail](https://content.abcam.com/products/images/nedd-9-casl-hef1-antibody-blr248l-bsa-free-ab314111--western-blot-img420708.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111)")
![Immunoprecipitation - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111), expandable thumbnail](https://content.abcam.com/products/images/nedd-9-casl-hef1-antibody-blr248l-bsa-free-ab314111--immunoprecipitation-img420709.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human NEDD9 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q14511
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Scaffolding protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (PubMed:24574519). As a focal adhesion protein, plays a role in embryonic fibroblast migration (By similarity). May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRKL and SHPTP2 to the tyrosine phosphorylated form (PubMed:9020138). Promotes adhesion and migration of lymphocytes; as a result required for the correct migration of lymphocytes to the spleen and other secondary lymphoid organs (PubMed:17174122). Plays a role in the organization of T-cell F-actin cortical cytoskeleton and the centralization of T-cell receptor microclusters at the immunological synapse (By similarity). Negatively regulates cilia outgrowth in polarized cysts (By similarity). Modulates cilia disassembly via activation of AURKA-mediated phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed:17604723). Positively regulates RANKL-induced osteoclastogenesis (By similarity). Required for the maintenance of hippocampal dendritic spines in the dentate gyrus and CA1 regions, thereby involved in spatial learning and memory (By similarity).
Alternative names
CASL, NEDD9, Enhancer of filamentation 1, hEF1, CRK-associated substrate-related protein, Cas scaffolding protein family member 2, Neural precursor cell expressed developmentally down-regulated protein 9, Renal carcinoma antigen NY-REN-12, p105, CAS-L, CasL, CASS2, NEDD-9
Recommended products
Rabbit Recombinant Monoclonal HEF1/NEDD-9 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NEDD9 aa 150-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human NEDD9 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q14511
- Clone number
- BLR248L
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HEF1 also known as NEDD9 is an intracellular signaling protein and a member of the Crk-associated substrate family. It has a molecular mass of approximately 93 kDa. The expression of this protein occurs mainly in tissues involved in cell movement and proliferation like lymphoid organs and neural tissues. It is part of the CAS (Crk-associated substrate) proteins hence associated with a wide range of cellular mechanics.
- Biological function summary
HEF1 functions as a scaffolding protein involved in various cellular processes including cell adhesion migration and proliferation. It interacts with several other proteins and forms part of complexes such as focal adhesion complexes. It regulates the dynamics of these complexes by modulating signaling pathways linked to cellular adhesion and migration. In neuronal cells it influences axonal and dendritic branching.
- Pathways
HEF1 plays a significant role in both the integrin signaling pathway and cell cycle regulation. Through these pathways HEF1 interacts with various key proteins including focal adhesion kinase (FAK) and Src kinase. It positions itself strategically within these pathways and facilitates cross-talk between signaling molecules enabling the coordination of structural and replicative functions in cells.
- Associated diseases and disorders
HEF1 has been implicated in the progression of cancers such as breast cancer and melanoma. Its overexpression often correlates with increased metastatic potential. In breast cancer HEF1 interacts with proteins like Aurora A and BRCA1 linking it to pathways involved in tumor proliferation and invasion. In melanoma alterations in HEF1 levels can affect melanoma cell migration making it a potential target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
![Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111), expandable thumbnail](https://content.abcam.com/products/images/nedd-9-casl-hef1-antibody-blr248l-bsa-free-ab314111--western-blot-img420707.jpg "Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111)")
Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111)
Samples:Whole cell lysate (50 µg) from OVCAR-8, U-251MG, A-549, and UACC-257 cells prepared using NETN lysis buffer. Antibody:Rabbit antiNEDD-9/CASL/HEF1 recombinant monoclonal antibody [BLR248L] (A700-248 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection:Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).
All lanes: Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111) at 1/1000 dilution
Lane 1: OVCAR-8 Whole cell lysate at 50 µg
Lane 2: U-251MG Whole cell lysate at 50 µg
Lane 3: A-549 Whole cell lysate at 50 µg
Lane 4: UACC-257 Whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
![Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111), expandable thumbnail](https://content.abcam.com/products/images/nedd-9-casl-hef1-antibody-blr248l-bsa-free-ab314111--western-blot-img420708.jpg "Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111)")
Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111)
Samples:Whole cell lysate (50 µg) from NIH 3T3 and C2C12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody [BLR248L] (A700-248 lot 1) used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection:Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).
All lanes: Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111) at 1/1000 dilution
Lane 1: NIH 3T3 Whole cell lysate at 50 µg
Lane 2: C2C12 Whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
![Immunoprecipitation - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111), expandable thumbnail](https://content.abcam.com/products/images/nedd-9-casl-hef1-antibody-blr248l-bsa-free-ab314111--immunoprecipitation-img420709.jpg "Immunoprecipitation - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111)")
Immunoprecipitation - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111)
Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies:Rabbit antiNEDD-9/CASL/HEF1 recombinant monoclonal antibody [BLR248L] (A700-248) used for IP at 6 µl/mg lysate. NEDD-9/CASL/HEF1 was also immunoprecipitated by a second antibody against a different epitope of NEDD-9/CASL/HEF1 (Antibody 2). For blotting immunoprecipitated NEDD-9/CASL/HEF1, A700-248 was used at 1:1000. Detection:Chemiluminescence with an exposure time of 30 seconds.
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