AB314111

Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal HEF1/NEDD-9 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NEDD9 aa 150-200.

View Alternative Names

CASL, NEDD9, Enhancer of filamentation 1, hEF1, CRK-associated substrate-related protein, Cas scaffolding protein family member 2, Neural precursor cell expressed developmentally down-regulated protein 9, Renal carcinoma antigen NY-REN-12, p105, CAS-L, CasL, CASS2, NEDD-9

3 Images

Supplier Data

Immunoprecipitation - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies : Rabbit antiNEDD-9/CASL/HEF1 recombinant monoclonal antibody [BLR248L] (A700-248) used for IP at 6 µl/mg lysate. NEDD-9/CASL/HEF1 was also immunoprecipitated by a second antibody against a different epitope of NEDD-9/CASL/HEF1 (Antibody 2). For blotting immunoprecipitated NEDD-9/CASL/HEF1, A700-248 was used at 1 : 1000. Detection : Chemiluminescence with an exposure time of 30 seconds.

false

Supplier Data

Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111)

Samples : Whole cell lysate (50 µg) from OVCAR-8, U-251MG, A-549, and UACC-257 cells prepared using NETN lysis buffer. Antibody : Rabbit antiNEDD-9/CASL/HEF1 recombinant monoclonal antibody [BLR248L] (A700-248 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection : Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).

All lanes:

Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111) at 1/1000 dilution

Lane 1:

OVCAR-8 Whole cell lysate at 50 µg

Lane 2:

U-251MG Whole cell lysate at 50 µg

Lane 3:

A-549 Whole cell lysate at 50 µg

Lane 4:

UACC-257 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Supplier Data

Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111)

Samples : Whole cell lysate (50 µg) from NIH 3T3 and C2C12 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody [BLR248L] (A700-248 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection : Chemiluminescence with an exposure time of 3 minutes. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).

All lanes:

Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (ab314111) at 1/1000 dilution

Lane 1:

NIH 3T3 Whole cell lysate at 50 µg

Lane 2:

C2C12 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR248L

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human NEDD9 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q14511

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEF1 also known as NEDD9 is an intracellular signaling protein and a member of the Crk-associated substrate family. It has a molecular mass of approximately 93 kDa. The expression of this protein occurs mainly in tissues involved in cell movement and proliferation like lymphoid organs and neural tissues. It is part of the CAS (Crk-associated substrate) proteins hence associated with a wide range of cellular mechanics.

Biological function summary

HEF1 functions as a scaffolding protein involved in various cellular processes including cell adhesion migration and proliferation. It interacts with several other proteins and forms part of complexes such as focal adhesion complexes. It regulates the dynamics of these complexes by modulating signaling pathways linked to cellular adhesion and migration. In neuronal cells it influences axonal and dendritic branching.

Pathways

HEF1 plays a significant role in both the integrin signaling pathway and cell cycle regulation. Through these pathways HEF1 interacts with various key proteins including focal adhesion kinase (FAK) and Src kinase. It positions itself strategically within these pathways and facilitates cross-talk between signaling molecules enabling the coordination of structural and replicative functions in cells.

HEF1 has been implicated in the progression of cancers such as breast cancer and melanoma. Its overexpression often correlates with increased metastatic potential. In breast cancer HEF1 interacts with proteins like Aurora A and BRCA1 linking it to pathways involved in tumor proliferation and invasion. In melanoma alterations in HEF1 levels can affect melanoma cell migration making it a potential target for therapeutic intervention.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Scaffolding protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (PubMed : 24574519). As a focal adhesion protein, plays a role in embryonic fibroblast migration (By similarity). May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRKL and SHPTP2 to the tyrosine phosphorylated form (PubMed : 9020138). Promotes adhesion and migration of lymphocytes; as a result required for the correct migration of lymphocytes to the spleen and other secondary lymphoid organs (PubMed : 17174122). Plays a role in the organization of T-cell F-actin cortical cytoskeleton and the centralization of T-cell receptor microclusters at the immunological synapse (By similarity). Negatively regulates cilia outgrowth in polarized cysts (By similarity). Modulates cilia disassembly via activation of AURKA-mediated phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed : 17604723). Positively regulates RANKL-induced osteoclastogenesis (By similarity). Required for the maintenance of hippocampal dendritic spines in the dentate gyrus and CA1 regions, thereby involved in spatial learning and memory (By similarity).

See full target information NEDD9

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free

Immunoprecipitation - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111)

Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111)

Western blot - Anti-NEDD-9/CASL/HEF1 antibody [BLR248L] - BSA free (AB314111)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise