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AB230201

Anti-NEDD1 antibody

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(1 Publication)

Rabbit Polyclonal NEDD1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Protein NEDD1 aa 400 to C-terminus.

View Alternative Names

Protein NEDD1, Neural precursor cell expressed developmentally down-regulated protein 1, NEDD-1, NEDD1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD1 antibody (AB230201)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD1 antibody (AB230201)

Paraffin-embedded human glioma cancer tissue stained for NEDD1 using ab230201 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-NEDD1 antibody (AB230201)
  • WB

Unknown

Western blot - Anti-NEDD1 antibody (AB230201)

Observed MW : 72kDa

All lanes:

Western blot - Anti-NEDD1 antibody (ab230201) at 1/2000 dilution

Lane 1:

Mouse liver tissue

Lane 2:

Mouse lung tissue

Lane 3:

Mouse kidney tissue

Lane 4:

293T cell lysate

Lane 5:

Hela cell lysate

Lane 6:

Jurkat cell lysate

Lane 7:

HepG-2 cell lysate

Lane 8:

A549 cell lysate

Lane 9:

PC-3 cell lysate

Predicted band size: 72 kDa

false

Immunoprecipitation - Anti-NEDD1 antibody (AB230201)
  • IP

Supplier Data

Immunoprecipitation - Anti-NEDD1 antibody (AB230201)

NEDD1 was immunoprecipitated from 500 μg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using 4 μg of ab230201.

Lane 1 : 1 μg Rabbit monoclonal IgG instead of ab230201 in HeLa whole cell lysate.

Lane 2 : ab230201 IP in HeLa whole cell lysate.

Lane 3 : HeLa whole cell lysate 20 μg (input).

For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.

All lanes:

Immunoprecipitation - Anti-NEDD1 antibody (ab230201)

Predicted band size: 72 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Protein NEDD1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NHV4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEDD1 also known as Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 1 plays a significant mechanical role in the assembly of the gamma-tubulin ring complex. It aids in microtubule nucleation. The protein with a molecular mass of approximately 72 kDa exhibits a strong expression in actively dividing cells particularly in tissues like the brain and testis. NEDD1 serves as an important marker for understanding cell cycle dynamics due to its influence on centrosome and spindle function.
Biological function summary

NEDD1 contributes to the formation and stabilization of microtubule-organizing centers. It is an essential component of the gamma-tubulin ring complex (γ-TuRC) which is integral for the nucleation of microtubules. The localization of NEDD1 to centrosomes influences spindle formation during cell division which is critical for accurate chromosomal segregation. This biological activity highlights the protein’s role in maintaining normal cellular architecture during proliferation.

Pathways

NEDD1 plays key roles in the cell cycle and cellular division pathways. It associates with the gamma-tubulin and other centrosomal proteins like TUBG1 to regulate microtubule formation. During mitosis these interactions ensure precise chromosomal alignment and segregation. In the broader context NEDD1's involvement in these pathways supports cellular roles in growth and response to external stimuli bridging understanding between molecular function and cellular outcomes.

Defects or misregulation in NEDD1 function can lead to conditions like cancer and neurodevelopmental disorders. Aberrant expression or mutation of NEDD1 contributes to centrosome amplification and aneuploidy which are hallmarks of tumorigenesis. In cancer its interaction with proteins like Aurora A kinase influences mitotic spindle assembly errors highlighting its potential as a therapeutic target. Moreover studying its dysfunction in neural tissue provides insights into developmental abnormalities and neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.
See full target information Protein NEDD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 46: PubMed34278506

2021

miR‑367‑3p downregulates Rab23 expression and inhibits Hedgehog signaling resulting in the inhibition of the proliferation, migration, and invasion of prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Du,Dong Li,Jianhao Xie,Ping Tang
View all publications
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