AB230254

Anti-NEDD1 antibody

Name: Anti-NEDD1 antibody (ab230254) | Abcam
Brand: Abcam Limited
SKU: AB230254
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal NEDD1 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Protein NEDD1 aa 400 to C-terminus.

View Alternative Names

Protein NEDD1, Neural precursor cell expressed developmentally down-regulated protein 1, NEDD-1, NEDD1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD1 antibody (AB230254)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD1 antibody (AB230254)

Paraffin-embedded human thyroid tissue stained for NEDD1 using ab230254 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD1 antibody (AB230254)

Paraffin-embedded human glioma cancer tissue stained for NEDD1 using ab230254 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-NEDD1 antibody (AB230254)

All lanes:

Western blot - Anti-NEDD1 antibody (ab230254) at 1/1000 dilution

Lane 1:

Mouse small intestine tissue lysate

Lane 2:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Secondary

All lanes:

Goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 72 kDa

Observed band size: 72 kDa

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Supplier Data

Immunoprecipitation - Anti-NEDD1 antibody (AB230254)

NEDD1 was immunoprecipitated from 0.5 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg ab230254. Western blot was performed from the immunoprecipitate using ab230254. An HRP-conjugated light chain specific antibody was used as secondary antibody at 1/50,000 dilution.

Lane 1 : Rabbit monoclonal IgG (1 μg) instead of ab230254 in HeLa whole cell lysate.

Lane 2 : ab230254 IP in HeLa whole cell lysate.

Lane 3 : HeLa whole cell lysate 20 μg (Input).

All lanes:

Immunoprecipitation - Anti-NEDD1 antibody (ab230254)

Predicted band size: 72 kDa

Observed band size: 72 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human Protein NEDD1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NHV4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEDD1 also known as Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 1 plays a significant mechanical role in the assembly of the gamma-tubulin ring complex. It aids in microtubule nucleation. The protein with a molecular mass of approximately 72 kDa exhibits a strong expression in actively dividing cells particularly in tissues like the brain and testis. NEDD1 serves as an important marker for understanding cell cycle dynamics due to its influence on centrosome and spindle function.

Biological function summary

NEDD1 contributes to the formation and stabilization of microtubule-organizing centers. It is an essential component of the gamma-tubulin ring complex (γ-TuRC) which is integral for the nucleation of microtubules. The localization of NEDD1 to centrosomes influences spindle formation during cell division which is critical for accurate chromosomal segregation. This biological activity highlights the protein’s role in maintaining normal cellular architecture during proliferation.

Pathways

NEDD1 plays key roles in the cell cycle and cellular division pathways. It associates with the gamma-tubulin and other centrosomal proteins like TUBG1 to regulate microtubule formation. During mitosis these interactions ensure precise chromosomal alignment and segregation. In the broader context NEDD1's involvement in these pathways supports cellular roles in growth and response to external stimuli bridging understanding between molecular function and cellular outcomes.

Defects or misregulation in NEDD1 function can lead to conditions like cancer and neurodevelopmental disorders. Aberrant expression or mutation of NEDD1 contributes to centrosome amplification and aneuploidy which are hallmarks of tumorigenesis. In cancer its interaction with proteins like Aurora A kinase influences mitotic spindle assembly errors highlighting its potential as a therapeutic target. Moreover studying its dysfunction in neural tissue provides insights into developmental abnormalities and neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.

See full target information Protein NEDD1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Diagnostic pathology 20:79 PubMed40604870

2025

SHMT2 overexpression improves glaucoma by enhancing mitophagy in retinal ganglion cells through promoting the phospho of PINK1.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Cui,Baojun Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NEDD1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD1 antibody (AB230254)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD1 antibody (AB230254)

Western blot - Anti-NEDD1 antibody (AB230254)

Immunoprecipitation - Anti-NEDD1 antibody (AB230254)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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