AB245522

Anti-NEDD4-2 antibody

Name: Anti-NEDD4-2 antibody (ab245522) | Abcam
Brand: Abcam Limited
SKU: AB245522
Price: 185 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal NEDD4-2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human NEDD4L aa 200-300.

View Alternative Names

KIAA0439, NEDL3, NEDD4L, E3 ubiquitin-protein ligase NEDD4-like, HECT-type E3 ubiquitin transferase NED4L, NEDD4.2, Nedd4-2

2 Images

Supplier Data

Immunoprecipitation - Anti-NEDD4-2 antibody (AB245522)

NEDD4-2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245522 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245522 at 0.4 μg/ml.

Lane 1 : ab245522 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-NEDD4-2 antibody (ab245522)

Predicted band size: 112 kDa

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Unknown

Western blot - Anti-NEDD4-2 antibody (AB245522)

All lanes:

Western blot - Anti-NEDD4-2 antibody (ab245522) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 112 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NEDD4L aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q96PU5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245522 was affinity purified using an epitope specific to NEDD4-2 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEDD4-2 also known as NEDD4L is a HECT domain-containing ubiquitin E3 ligase with a mass of approximately 120 kDa. It is expressed in various tissues especially in the kidney lung and colon. NEDD4-2 is involved in tagging proteins with ubiquitin marking them for degradation via the proteasome pathway. This protein helps regulate protein turnover by attaching ubiquitin chains to specific substrate proteins.

Biological function summary

NEDD4-2 regulates key cellular processes by controlling the abundance of membrane proteins like ion channels and receptors. It binds and ubiquitinates specific targets like ENaC (epithelial sodium channel) significantly influencing electrolyte and fluid balance. NEDD4-2 often functions as part of multiprotein complexes that modulate cellular homeostasis. Efficient function of these complexes ensures cells maintain balance in their environment.

Pathways

NEDD4-2 integrates into the MAPK and Wnt signaling pathways influencing diverse cellular outcomes like proliferation and apoptosis. Acting within these pathways NEDD4-2 associates with proteins such as Ras and β-catenin. This interaction influences cellular responses to external stimuli playing roles in cellular growth regulation and fate decisions.

Abnormalities in NEDD4-2 function are linked to conditions like Liddle's syndrome and hypertension. Mutations or dysregulation in NEDD4-2 can alter ENaC activity leading to increased sodium retention and contribute to high blood pressure. Moreover it connects to disorders like epithelial cancers through pathways involving β-catenin indicating its potential role in tumorigenesis. Research continues to explore these interactions to identify therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair (PubMed : 20064473, PubMed : 31959741, PubMed : 33608556). Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation (PubMed : 15496141). Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels (PubMed : 28820317, PubMed : 31959741). Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5 (PubMed : 26363003, PubMed : 27445338). Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed : 25631046). Plays a role in dendrite formation by melanocytes (PubMed : 23999003). Involved in the regulation of TOR signaling (PubMed : 27694961). Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF (PubMed : 27445338). Plays a role in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, resulting in enhanced 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3 (PubMed : 33608556). Ubiquitinates TTYH2 and TTYH3 and regulates protein levels of TTYH2 (PubMed : 18577513).

See full target information NEDD4L

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:579 PubMed40744915

2025

PRMT1-mediated methylation of UBE2m promoting calcium oxalate crystal-induced kidney injury by inhibiting fatty acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhui Yuan,Zehua Ye,Shuqin Mei,Miao Zhang,Ming Wu,Fangyou Lin,Weimin Yu,Wei Li,Xiangjun Zhou,Fan Cheng

Scientific reports 15:11486 PubMed40181096

2025

Glucagon like peptide-1 modulates urinary sodium excretion in diabetic kidney disease via ENaC activation.

Applications

Unspecified application

Species

Unspecified reactive species

Goh Kodama,Kensei Taguchi,Sakuya Ito,Yuri Nishino,Kayo Mori,Satomi Taguchi,Yuta Mitsuishi,Yuya Yamashita,Sho-Ichi Yamagishi,Kei Fukami

Communications biology 8:159 PubMed39901028

2025

Arginine methyltransferase PRMT1 promotes ferroptosis through EGR1/GLS2 axis in sepsis-related acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Longhui Hu,Qiao Ke,Zhao Li,Chujun Ruan,Hanjing Lu,Xiaoran Liu

Calcified tissue international 115:673-685 PubMed39198270

2024

Morinda Officinalis Polysaccharides Inhibit Osteoclast Differentiation by Regulating miR-214-3p/NEDD4L in Postmenopausal Osteoporosis Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Huang,Jian Chen,Xiaomei Lin,Zhengkun Lin

Scientific reports 13:17282 PubMed37828032

2023

LINC01343 targets miR-526b-5p to facilitate the development of hepatocellular carcinoma by upregulating ROBO1.

Applications

Unspecified application

Species

Unspecified reactive species

Song Wu,Tao Tang,Hongchi Zhou,Jing Huang,Xiaoliang Kang,Junli Zhang

Stem cells international 2023:7545635 PubMed36644009

2023

Molecular Mechanism of Long Noncoding RNA SNHG14 in Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells through the NEDD4L/FOXA2/PCP4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Han Wang,Mingxing Fan,Yan An,Da He

Cancer biology & therapy 23:243-253 PubMed35316145

2022

NEDD4L inhibits glycolysis and proliferation of cancer cells in oral squamous cell carcinoma by inducing ENO1 ubiquitination and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Guangping Zhang,Xin Zhao,Weixian Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NEDD4-2 antibody

Immunoprecipitation - Anti-NEDD4-2 antibody (AB245522)

Western blot - Anti-NEDD4-2 antibody (AB245522)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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