JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB168349

Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)]

Be the first to review this product! Submit a review

|

(14 Publications)

Rabbit Recombinant Monoclonal NEDD4-2 phospho S448 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 14 publications.

View Alternative Names

KIAA0439, NEDL3, NEDD4L, E3 ubiquitin-protein ligase NEDD4-like, HECT-type E3 ubiquitin transferase NED4L, NEDD4.2, Nedd4-2

2 Images
Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (AB168349)
  • IP

Unknown

Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (AB168349)

Detection of NEDD4-2 by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168349 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349)

Predicted band size: 112 kDa

false

Western blot - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (AB168349)
  • WB

Unknown

Western blot - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (AB168349)

All lanes:

Western blot - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (ab168349) at 1/1000 dilution

Lane 1:

293T cell lysate, untreated at 10 µg

Lane 2:

293T cell lysate, treated with Lambda Phosphatase at 10 µg

Predicted band size: 112 kDa

Observed band size: 110-135 kDa

false

  • Carrier free

    Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8269(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB95399

Anti-NEDD4-2 (phospho S328) antibody

  • 5
  • 1
Primary Antibodies

AB46521

Anti-NEDD4-2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD4-2 antibody (AB46521)

  • 3
  • 6
Primary Antibodies

AB240753

Anti-NEDD4-2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD4-2 antibody (AB240753)

  • 0
  • 0
Primary Antibodies

AB124643

Anti-NEDD4-2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDD4-2 antibody (AB124643)

  • 0
  • 0
Primary Antibodies

AB245522

Anti-NEDD4-2 antibody

Immunoprecipitation - Anti-NEDD4-2 antibody (AB245522)

  • 5
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB117826

Anti-NEDD4 antibody

Immunoprecipitation - Anti-NEDD4 antibody (AB117826)

  • 4
  • 1
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEDD4-2 also known as NEDD4L is a HECT domain-containing ubiquitin E3 ligase with a mass of approximately 120 kDa. It is expressed in various tissues especially in the kidney lung and colon. NEDD4-2 is involved in tagging proteins with ubiquitin marking them for degradation via the proteasome pathway. This protein helps regulate protein turnover by attaching ubiquitin chains to specific substrate proteins.
Biological function summary

NEDD4-2 regulates key cellular processes by controlling the abundance of membrane proteins like ion channels and receptors. It binds and ubiquitinates specific targets like ENaC (epithelial sodium channel) significantly influencing electrolyte and fluid balance. NEDD4-2 often functions as part of multiprotein complexes that modulate cellular homeostasis. Efficient function of these complexes ensures cells maintain balance in their environment.

Pathways

NEDD4-2 integrates into the MAPK and Wnt signaling pathways influencing diverse cellular outcomes like proliferation and apoptosis. Acting within these pathways NEDD4-2 associates with proteins such as Ras and β-catenin. This interaction influences cellular responses to external stimuli playing roles in cellular growth regulation and fate decisions.

Abnormalities in NEDD4-2 function are linked to conditions like Liddle's syndrome and hypertension. Mutations or dysregulation in NEDD4-2 can alter ENaC activity leading to increased sodium retention and contribute to high blood pressure. Moreover it connects to disorders like epithelial cancers through pathways involving β-catenin indicating its potential role in tumorigenesis. Research continues to explore these interactions to identify therapeutic targets.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair (PubMed : 20064473, PubMed : 31959741, PubMed : 33608556). Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation (PubMed : 15496141). Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels (PubMed : 28820317, PubMed : 31959741). Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5 (PubMed : 26363003, PubMed : 27445338). Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed : 25631046). Plays a role in dendrite formation by melanocytes (PubMed : 23999003). Involved in the regulation of TOR signaling (PubMed : 27694961). Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF (PubMed : 27445338). Plays a role in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, resulting in enhanced 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3 (PubMed : 33608556). Ubiquitinates TTYH2 and TTYH3 and regulates protein levels of TTYH2 (PubMed : 18577513).
See full target information NEDD4L phospho S448
style
left-column

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 32:26 PubMed39972304

2025

K27-linked RORγt ubiquitination by Nedd4 potentiates Th17-mediated autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuming Zeng,Hui Guo,Na Tang,Pranav S Renavikar,Nitin J Karandikar,Amy E Lovett-Racke,Michael K Racke,Chengkai Yan,Rong Tang,Sushmita Sinha,Krishnendu Ghosh,Jeremy P Ryal,Song Ouyang,Min Chen,Foued Amari,Coppola Vincenzo,R Marshall Pope,Yalan Li,Huan Yang,Wallace Y Langdon,Jian Zhang

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 174:116447 PubMed38518606

2024

Inhibition the ubiquitination of ENaC and Na,K-ATPase with erythropoietin promotes alveolar fluid clearance in sepsis-induced acute respiratory distress syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Gao,Fei Cao,Xinyi Tian,Qianping Zhang,Congcong Xu,Bowen Ji,Ye-An Zhang,Linan Du,Jun Han,Li Li,Siyu Zhou,Yuqiang Gong,Binyu Ying,Fang Gao-Smith,Shengwei Jin

Fluids and barriers of the CNS 20:61 PubMed37596666

2023

Inhibition of serum- and glucocorticoid-induced kinase 1 ameliorates hydrocephalus in preclinical models.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Hochstetler,Hillary Smith,Makenna Reed,Louise Hulme,Paul Territo,Amanda Bedwell,Scott Persohn,Nicola Perrotti,Lucia D'Antona,Francesca Musumeci,Silvia Schenone,Bonnie L Blazer-Yost

Endocrine connections 12: PubMed36662677

2023

NEDD4L facilitates granulosa cell ferroptosis by promoting GPX4 ubiquitination and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Tang,Xiaomei Jiang,Yu Hua,Heyue Li,Chunlan Zhu,Xiaobai Hao,Minhui Yi,Linxia Li

Frontiers in pharmacology 13:970812 PubMed36278222

2022

Aldosterone-stimulated endothelial epithelial sodium channel (EnNaC) plays a role in cold exposure-induced hypertension in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Liang-Liang Tang,Xu Yang,Shu-Qi Yu,Qi Qin,Rong Xue,Yu Sun,Han Xiao,An-Qi Shang,Jia-Qun Liu,Song-Qi Han,Chen Liang,Jie Lou,Qiu-Shi Wang,Chang-Jiang Yu,Ming-Ming Wu,Zhi-Ren Zhang

Frontiers in pharmacology 13:942769 PubMed36059970

2022

Pharmacological suppression of Nedd4-2 rescues the reduction of Kv11.1 channels in pathological cardiac hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Zhang,Tian Fu,Jinglei Sun,Sihao Zou,Suhua Qiu,Jiali Zhang,Shi Su,Chenxia Shi,De-Pei Li,Yanfang Xu

Biomedicines 9: PubMed34440273

2021

AMPA Receptor Antagonists Facilitate NEDD4-2-Mediated GRIA1 Ubiquitination by Regulating PP2B-ERK1/2-SGK1 Pathway in Chronic Epilepsy Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Eun Kim,Duk-Shin Lee,Hana Park,Tae-Hyun Kim,Tae-Cheon Kang

Frontiers in cell and developmental biology 9:672335 PubMed34222246

2021

Homocysteine Causes Endothelial Dysfunction via Inflammatory Factor-Mediated Activation of Epithelial Sodium Channel (ENaC).

Applications

Unspecified application

Species

Unspecified reactive species

Chen Liang,Qiu-Shi Wang,Xu Yang,Di Zhu,Yu Sun,Na Niu,Jie Yao,Bi-Han Dong,Shuai Jiang,Liang-Liang Tang,Jie Lou,Chang-Jiang Yu,Qun Shao,Ming-Ming Wu,Zhi-Ren Zhang

Frontiers in pharmacology 12:665111 PubMed34122084

2021

NaHS or Lovastatin Attenuates Cyclosporine A-Induced Hypertension in Rats by Inhibiting Epithelial Sodium Channels.

Applications

Unspecified application

Species

Unspecified reactive species

Qiu-Shi Wang,Chen Liang,Shuai Jiang,Di Zhu,Yu Sun,Na Niu,Xu Yang,Yan-Chao Yang,Bi-Han Dong,Jie Yao,Chang-Jiang Yu,Jie Lou,Liang-Liang Tang,Ming-Ming Wu,Zhi-Ren Zhang,He-Ping Ma

Oxidative medicine and cellular longevity 2020:3921897 PubMed33194000

2020

Stimulation of Epithelial Sodium Channels in Endothelial Cells by Bone Morphogenetic Protein-4 Contributes to Salt-Sensitive Hypertension in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Yang,Na Niu,Chen Liang,Ming-Ming Wu,Liang-Liang Tang,Qiu-Shi Wang,Jie Lou,Bin-Lin Song,Wei-Wan Zheng,He-Ping Ma,Zhi-Ren Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com