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AB249448

Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal NEDD4-2 phospho S448 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

KIAA0439, NEDL3, NEDD4L, E3 ubiquitin-protein ligase NEDD4-like, HECT-type E3 ubiquitin transferase NED4L, NEDD4.2, Nedd4-2

2 Images
Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] - BSA and Azide free (AB249448)
  • IP

Unknown

Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] - BSA and Azide free (AB249448)

This data was developed using ab168349, the same antibody clone in a different buffer formulation.

Detection of NEDD4-2 by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168349 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (<a href='/en-us/products/primary-antibodies/nedd4-2-phospho-s448-antibody-epr82692-ab168349'>ab168349</a>)

Predicted band size: 112 kDa

false

Western blot - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] - BSA and Azide free (AB249448)
  • WB

Unknown

Western blot - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] - BSA and Azide free (AB249448)

This data was developed using ab168349, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] (<a href='/en-us/products/primary-antibodies/nedd4-2-phospho-s448-antibody-epr82692-ab168349'>ab168349</a>) at 1/1000 dilution

Lane 1:

293T cell lysate, untreated at 10 µg

Lane 2:

293T cell lysate, treated with Lambda Phosphatase at 10 µg

Predicted band size: 112 kDa

Observed band size: 110-135 kDa

false

  • Unconjugated

    Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8269(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249448 is the carrier-free version of ab168349.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEDD4-2 also known as NEDD4L is a HECT domain-containing ubiquitin E3 ligase with a mass of approximately 120 kDa. It is expressed in various tissues especially in the kidney lung and colon. NEDD4-2 is involved in tagging proteins with ubiquitin marking them for degradation via the proteasome pathway. This protein helps regulate protein turnover by attaching ubiquitin chains to specific substrate proteins.
Biological function summary

NEDD4-2 regulates key cellular processes by controlling the abundance of membrane proteins like ion channels and receptors. It binds and ubiquitinates specific targets like ENaC (epithelial sodium channel) significantly influencing electrolyte and fluid balance. NEDD4-2 often functions as part of multiprotein complexes that modulate cellular homeostasis. Efficient function of these complexes ensures cells maintain balance in their environment.

Pathways

NEDD4-2 integrates into the MAPK and Wnt signaling pathways influencing diverse cellular outcomes like proliferation and apoptosis. Acting within these pathways NEDD4-2 associates with proteins such as Ras and β-catenin. This interaction influences cellular responses to external stimuli playing roles in cellular growth regulation and fate decisions.

Abnormalities in NEDD4-2 function are linked to conditions like Liddle's syndrome and hypertension. Mutations or dysregulation in NEDD4-2 can alter ENaC activity leading to increased sodium retention and contribute to high blood pressure. Moreover it connects to disorders like epithelial cancers through pathways involving β-catenin indicating its potential role in tumorigenesis. Research continues to explore these interactions to identify therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair (PubMed : 20064473, PubMed : 31959741, PubMed : 33608556). Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation (PubMed : 15496141). Downregulates autophagy and cell growth by ubiquitinating and reducing cellular ULK1 or ASCT2 levels (PubMed : 28820317, PubMed : 31959741). Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5 (PubMed : 26363003, PubMed : 27445338). Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed : 25631046). Plays a role in dendrite formation by melanocytes (PubMed : 23999003). Involved in the regulation of TOR signaling (PubMed : 27694961). Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF (PubMed : 27445338). Plays a role in antiviral innate immunity by catalyzing 'Lys-29'-linked cysteine ubiquitination of TRAF3, resulting in enhanced 'Lys-48' and 'Lys-63'-linked ubiquitination of TRAF3 (PubMed : 33608556). Ubiquitinates TTYH2 and TTYH3 and regulates protein levels of TTYH2 (PubMed : 18577513).
See full target information NEDD4L phospho S448
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Product promise

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