Rabbit Polyclonal NEDD8 antibody. Suitable for WB and reacts with Recombinant full length protein - Saccharomyces cerevisiae, Saccharomyces cerevisiae samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NEDD8.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human NEDD8. Database link Q15843
Reactivity data
Select an application| WB | |
|---|---|
| Recombinant full length protein - Saccharomyces cerevisiae | Tested |
| Saccharomyces cerevisiae | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Saccharomyces cerevisiae | Dilution info 1/500 - 1/2000 | Notes - |
Species Saccharomyces cerevisiae | Dilution info 1/500 - 1/2000 | Notes - |
Associated Products
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Target data
Function
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53 (PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:14690597, PubMed:15242646, PubMed:9694792, PubMed:38605244). Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins (PubMed:10318914, PubMed:10597293, PubMed:11953428, PubMed:20688984, PubMed:9694792, PubMed:38605244). Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity (PubMed:15242646). Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M (PubMed:14690597).
Alternative names
Ubiquitin-like protein NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8, NEDD8
Recommended products
Rabbit Polyclonal NEDD8 antibody. Suitable for WB and reacts with Recombinant full length protein - Saccharomyces cerevisiae, Saccharomyces cerevisiae samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NEDD8.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human NEDD8. Database link Q15843
- Purification technique
- Ion exchange chromatography
- Concentration
- Purification notes
Rub1 Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NEDD8 also known as neural precursor cell expressed developmentally downregulated protein 8 is a small ubiquitin-like protein with a molecular weight of approximately 9 kDa. It is expressed in various tissues prominently in the brain and muscles. NEDD8 conjugates to target proteins modifying their functions by altering their stability activity or location. It plays a role in the regulation of protein degradation.
- Biological function summary
The NEDD8 protein is part of a modification system that activates cullin-RING ligase complexes. This activation promotes the ubiquitination and subsequent proteasomal degradation of targeted proteins. As a post-translational modification system similar to ubiquitin NEDD8 is important for maintaining protein homeostasis by ensuring timely degradation and turnover of cellular proteins. These processes impact cell cycle progression signal transduction and stress responses.
- Pathways
NEDD8 is a central player in the ubiquitin-proteasome system an essential pathway for protein turnover. It is particularly important in the NEDDylation pathway where it regulates protein functions by transiently attaching to substrate proteins. Through these processes NEDD8 interacts closely with proteins like cullins and ubiquitin affecting cellular responses and maintaining cellular integrity under different conditions.
- Associated diseases and disorders
NEDD8 influences several pathological states. Aberrant NEDD8 conjugation can lead to cancer by disrupting the controlled degradation of proteins involved in cell cycle regulation such as p27 and p21. Additionally improper NEDDylation has links to neurodegenerative diseases like Alzheimer's as it affects proteins related to synaptic function and neuronal survival. Understanding the role of NEDD8 in these diseases aids in the development of potential therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Nedd8 antibody (ab4751)
ab4751 tested by immunoblot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of Donkey polyclonal to Rabbit IgG (HRP) (Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed ab7083) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). ab4751 tested by immunoblot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of Donkey polyclonal to Rabbit IgG (HRP) (Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed ab7083) for 1 hour at r
All lanes: Western blot - Anti-Nedd8 antibody (ab4751)
Predicted band size: 9 kDa
Western blot - Anti-Nedd8 antibody (ab4751)
Immunoblot of Rub1 fusion protein. Anti-Rub1 antibody generated by immunization with recombinant yeast Rub1 was tested by immunoblot against yeast lysates expressing the Rub1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of Goat polyclonal to GFP (Anti-GFP antibody ab6673) followed by reaction with a 1:15,000 dilution of Donkey polyclonal to Goat IgG (Donkey Anti-Goat IgG H&L (HRP) preadsorbed ab7125). Panel C shows specific reaction with Rub1 using a 1:1,000 dilution of ab4751 followed by reaction with a 1:15,000 dilution of Goat polyclonal to Rabbit IgG (HRP) (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat mi
All lanes: Western blot - Anti-Nedd8 antibody (ab4751)
Predicted band size: 9 kDa
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