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AB121264

Anti-NEDL1 antibody

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(2 Publications)

Rabbit Polyclonal NEDL1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HECW1 aa 500-650.

View Alternative Names

KIAA0322, NEDL1, HECW1, E3 ubiquitin-protein ligase HECW1, HECT-type E3 ubiquitin transferase HECW1, NEDD4-like E3 ubiquitin-protein ligase 1, hNEDL1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NEDL1 antibody (AB121264)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NEDL1 antibody (AB121264)

ab121264, at 4 μg/ml, staining NEDL1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence, showing positivity in nucleus but not nucleoli & cytoplasm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDL1 antibody (AB121264)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDL1 antibody (AB121264)

ab121264, at 1/15 dilution, staining NEDL1 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry, showing strong nuclear and cytoplasmic positivity in neuronal cells.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HECW1 aa 500-650. The exact immunogen used to generate this antibody is proprietary information.

Q76N89

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p>Perform Heat Induced Epitope Retrieval (HIER) at pH 6 using a pressure boiler as heat source.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fix cells with PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEDD4-like ubiquitin protein ligase 1 also known as NEDL1 is a protein known for its E3 ubiquitin ligase activity contributing to protein degradation and regulation processes. It has a mass of approximately 120 kDa. NEDL1 is expressed in various tissues including the brain and muscle where it influences multiple cellular mechanisms. It interacts with other proteins to promote ubiquitination which marks proteins for proteasomal degradation.
Biological function summary

NEDL1 participates in critical cellular processes like proteostasis by functioning as a part of larger protein complexes. It regulates the levels of specific proteins within cells affecting activities such as cell growth and apoptosis. Additionally NEDL1 influences cellular signaling pathways highlighting its role in maintaining cellular homeostasis and response to environmental changes.

Pathways

NEDL1 plays a significant role in the ubiquitin-proteasome pathway and interacts closely with proteins like p53 and SMAD to regulate their stability and activity. It is important in the TGF-beta signaling pathway where it affects the degradation of SMAD proteins. This interaction helps control the transcriptional responses that SMAD proteins mediate impacting processes like cell differentiation and growth.

NEDL1 has been linked to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) where it potentially interacts with proteins like TDP-43. The malfunction or mutation of NEDL1 can contribute to the accumulation of ubiquitinated protein aggregates which are characteristic of some neurodegenerative disorders. Furthermore disruptions in NEDL1 activity can also associate with muscular dystrophies where its functional imbalance might affect muscle cell health and maintenance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1. Also targets the mutant SOD1 protein involved in familial amyotrophic lateral sclerosis (FALS). Forms cytotoxic aggregates with DVL1, SSR3 and mutant SOD1 that lead to motor neuron death in FALS.
See full target information HECW1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 21:890 PubMed34348693

2021

Integrating HECW1 expression into the clinical indicators exhibits high accuracy in assessing the prognosis of patients with clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wang,Keqin Dong,Yuning Wang,Guang Peng,Xu Song,Yongwei Yu,Pei Shen,Xingang Cui

Oncology letters 11:1486-1492 PubMed26893765

2016

Novel somatic mutations identified by whole-exome sequencing in muscle-invasive transitional cell carcinoma of the bladder.

Applications

Unspecified application

Species

Unspecified reactive species

Huixing Pan,Xiaojian Xu,Deyao Wu,Qiaocheng Qiu,Shoujun Zhou,Xuefeng He,Yunfeng Zhou,Ping Qu,Jianquan Hou,Jun He,Jian Zhou
View all publications
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Product promise

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