Rabbit Polyclonal NEDL2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HECW2 aa 450-650.
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KIAA1301, NEDL2, HECW2, E3 ubiquitin-protein ligase HECW2, HECT-type E3 ubiquitin transferase HECW2, NEDD4-like E3 ubiquitin-protein ligase 2
- ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-NEDL2 antibody (AB236784)
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling NEDL2 (green) using ab236784 at 1/166 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDL2 antibody (AB236784)
Paraffin-embedded human brain tissue stained for NEDL2 using ab236784 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEDL2 antibody (AB236784)
Paraffin-embedded human glioma tissue stained for NEDL2 using ab236784 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Biological function summary
NEDL2 mediates protein ubiquitination a process influencing protein stability and function. NEDL2 associates with cellular signaling pathways impacting cellular homeostasis and stress responses. It functions independently but can also interact with other protein complexes to modulate their activities. The ubiquitination process controlled by NEDL2 contributes to the cellular regulation by targeting specific proteins for degradation thereby affecting various cellular processes.
Pathways
NEDL2 integrates into the ubiquitin-proteasome pathway a core mechanism for controlled protein degradation. It interacts with proteins like E2 ubiquitin-conjugating enzymes to perform its function. Another critical pathway that involves NEDL2 is the Wnt signaling pathway where ubiquitination processes influence signal transduction. NEDL2's interactions help maintain proper cellular function by regulating protein levels ensuring correct pathway activation or inhibition.
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Publications (1)
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iScience 27:110077 PubMed39040052
2024
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