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AB92711

Anti-NEDL2 antibody

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(3 Publications)

Rabbit Polyclonal NEDL2 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HECW2.

View Alternative Names

KIAA1301, NEDL2, HECW2, E3 ubiquitin-protein ligase HECW2, HECT-type E3 ubiquitin transferase HECW2, NEDD4-like E3 ubiquitin-protein ligase 2

1 Images
Western blot - Anti-NEDL2 antibody (AB92711)
  • WB

Unknown

Western blot - Anti-NEDL2 antibody (AB92711)

All lanes:

Western blot - Anti-NEDL2 antibody (ab92711) at 1/500 dilution

Lane 1:

HepG2 cell extracts at 30 µg

Lane 2:

LOVO cell extracts at 30 µg

Lane 3:

LOVO cell extracts at 30 µg with immunizing peptide

Predicted band size: 176 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human HECW2. The exact immunogen used to generate this antibody is proprietary information.

Q9P2P5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEDD4 E3 ubiquitin protein ligase-like 2 commonly known as NEDL2 functions as an E3 ubiquitin ligase. It plays a role in protein degradation through the ubiquitin-proteasome system. NEDL2 is approximately 130 kDa in mass. It is expressed in various tissues with higher levels detected in the brain and testis. NEDL2 facilitates the transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate protein marking it for degradation or regulating its activity.
Biological function summary

NEDL2 mediates protein ubiquitination a process influencing protein stability and function. NEDL2 associates with cellular signaling pathways impacting cellular homeostasis and stress responses. It functions independently but can also interact with other protein complexes to modulate their activities. The ubiquitination process controlled by NEDL2 contributes to the cellular regulation by targeting specific proteins for degradation thereby affecting various cellular processes.

Pathways

NEDL2 integrates into the ubiquitin-proteasome pathway a core mechanism for controlled protein degradation. It interacts with proteins like E2 ubiquitin-conjugating enzymes to perform its function. Another critical pathway that involves NEDL2 is the Wnt signaling pathway where ubiquitination processes influence signal transduction. NEDL2's interactions help maintain proper cellular function by regulating protein levels ensuring correct pathway activation or inhibition.

NEDL2 has links to neurodegenerative diseases and cancer. Its dysfunction may disrupt protein homeostasis contributing to conditions like Alzheimer's disease. In such cases improper ubiquitination can lead to the accumulation of misfolded proteins exacerbating disease progression. Additionally NEDL2's interactions with tumor suppressor proteins imply its potential role in oncogenesis. Altered NEDL2 activity might affect tumor suppressor degradation influencing cancer development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73 (PubMed : 12890487). Involved in the regulation of mitotic metaphase/anaphase transition (PubMed : 24163370).
See full target information HECW2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 14:2820-2832 PubMed37781079

2023

HECW2 promotes the progression and chemoresistance of colorectal cancer via AKT/mTOR signaling activation by mediating the ubiquitin-proteasome degradation of lamin B1.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Li,Li Wang,Yujue Wang,Hui Shen,Qianrui Kou,Changjun Shen,Xiangrong Xu,Yunqing Zhang,Jing Zhang

Biology of reproduction 104:117-129 PubMed33030211

2020

NEDD4-like ubiquitin ligase 2 protein (NEDL2) in porcine spermatozoa, oocytes, and preimplantation embryos and its role in oocyte fertilization†.

Applications

Unspecified application

Species

Unspecified reactive species

Jiude Mao,Michal Zigo,Dalen Zuidema,Miriam Sutovsky,Peter Sutovsky

Oncotarget 7:31440-53 PubMed27119228

2016

NEDL2 regulates enteric nervous system and kidney development in its Nedd8 ligase activity-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Qiu,Rongfei Wei,Yang Li,Qiong Zhu,Cong Xiong,Yuhan Chen,Yuan Zhang,Kefeng Lu,Fuchu He,Lingqiang Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB236784

Anti-NEDL2 antibody

primary-antibodies

nedl2-antibody-ab236784

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